Hi guys,
i wrote a little program to display filter sets used for microscopy. The
spectra data are shown using matplotlib and wxmpl. The GUI hat a menu item
"Set Lines" --> an extra Frame with checkboxes will open up.
Upon checking or unchecking, the laser lines should be displayed or deleted
inside the main panel as vertical lines, but I juts can not figure out, how
to acess the meain panel when checking.
The problem is located in line 58, 63 and 68
Here is the code:
#!/usr/bin/env python
import numpy
from pylab import *
import scipy
import scipy.linalg
import wx
import wxmpl
import os, sys
xmin = 350
xmax = 700
ymin = 0
ymax = 1.05
# normalizes dye spectra to 1
def normspec(datain):
out = datain
out[:,1] = datain[:,1]/datain[:,1].max(0)
return out
# converts filters spectra to 0-1 range
def normspec_filter(datain):
out = datain
if datain[:,1].max(0)>10:
out[:,1] = datain[:,1]/100
return out
class MyApp(wx.App):
def OnInit(self):
self.frame = MyFrame(None, -1, 'FilterSpectra')
self.frame.Show(True)
return True
class SetLaser(wx.Frame):
def __init__(self, parent, id, title):
wx.Frame.__init__(self, parent, id, title, size=(220, 130))
wl = array([405, 445, 473])
panel = wx.Panel(self, -1)
self.cb0 = wx.CheckBox(panel, 10, str(wl[0])+'nm', (10, 10))
self.cb1 = wx.CheckBox(panel, 11, str(wl[1])+'nm', (80, 10))
self.cb2 = wx.CheckBox(panel, 12, str(wl[2])+'nm', (150, 10))
self.Bind(wx.EVT_CHECKBOX, self.on_checkbox0, id=10)
self.Bind(wx.EVT_CHECKBOX, self.on_checkbox1, id=11)
self.Bind(wx.EVT_CHECKBOX, self.on_checkbox2, id=12)
self.CenterOnParent()
self.Show()
def on_checkbox0(self, event):
self.cb0value = event.IsChecked()
print self.cb0value
self.glaser0, = self.axes.plot([wl[i],wl[i]],[0,1],'b', lw=2)
def on_checkbox1(self, event):
self.cb1value = event.IsChecked()
print self.cb1value
self.glaser1, = self.axes.plot([wl[i],wl[i]],[0,1],'b', lw=2)
def on_checkbox2(self, event):
self.cb2value = event.IsChecked()
print self.cb2value
self.glaser2, = self.axes.plot([wl[i],wl[i]],[0,1],'b', lw=2)
class MyFrame(wx.Frame):
def __init__(self, parent, id, title):
wx.Frame.__init__(self, parent, id, title, size=(1000, 800))
menuBar = wx.MenuBar()
self.CreateStatusBar()
#--------------------------------------------------------------
file = wx.Menu()
file.Append(100, 'Save As ...', 'Saves Figure as ...')
file.AppendSeparator()
file.Append(113, '&Quit\tCtrl+W')
#--------------------------------------------------------------
dyes = wx.Menu()
dyes.Append(101, '&Load Dye Absorption', 'Loads Absorption Spectra
(*.abs)')
dyes.Append(102, '&Load Dye Fluorescence', 'Loads Fluorescence
Emission Spectra (*.flu)')
dyes.AppendSeparator()
dyes.Append(111, '&Delete last Absorption', 'Deletes last Absorption
Spectra')
dyes.Append(112, '&Delete last Fluorescence', 'Deletes last
Fluorescence Emission')
#--------------------------------------------------------------
filter = wx.Menu()
filter.Append(103, '&Load Excitation Filter', 'Loads Excitation
Filter (*.ex)')
filter.Append(104, '&Load Dichroic Mirror', 'Loads Dichroic Mirror
(*.di)')
filter.Append(105, '&Load Emission Filter', 'Loads Emission Filter
(*.em)')
filter.AppendSeparator()
filter.Append(107, '&Delete last EX filter', 'Deletes the last EX
filter')
filter.Append(108, '&Delete last DI mirror', 'Deletes the last DI
mirror')
filter.Append(109, '&Delete last EM filter', 'Deletes the last EM
filter')
#--------------------------------------------------------------
lightsource = wx.Menu()
lightsource.Append(106, '&Load Light Source', 'Loads Spectral Data
of Light Source (*.txt)')
lightsource.AppendSeparator()
lightsource.Append(110, '&Delete last Light Source', 'Deletes the
last Light Source')
#--------------------------------------------------------------
laserline = wx.Menu()
laserline.Append(114, '&Set Laser Lines',' Displays the selected
laser lines')
#--------------------------------------------------------------
menuBar.Append(file, '&File')
menuBar.Append(dyes, '&Dyes')
menuBar.Append(filter, '&Filter')
menuBar.Append(lightsource, '&Light Source')
menuBar.Append(laserline, '&Laser Lines')
#--------------------------------------------------------------
self.SetMenuBar(menuBar)
#--------------------------------------------------------------
self.Bind(wx.EVT_MENU, self.saveas, id = 100)
self.Bind(wx.EVT_MENU, self.openabs, id = 101)
self.Bind(wx.EVT_MENU, self.openflu, id = 102)
self.Bind(wx.EVT_MENU, self.openex, id = 103)
self.Bind(wx.EVT_MENU, self.opendi, id = 104)
self.Bind(wx.EVT_MENU, self.openem, id = 105)
self.Bind(wx.EVT_MENU, self.openls, id = 106)
self.Bind(wx.EVT_MENU, self.delex, id = 107)
self.Bind(wx.EVT_MENU, self.deldi, id = 108)
self.Bind(wx.EVT_MENU, self.delem, id = 109)
self.Bind(wx.EVT_MENU, self.dells, id = 110)
self.Bind(wx.EVT_MENU, self.delabs, id = 111)
self.Bind(wx.EVT_MENU, self.delflu, id = 112)
self.Bind(wx.EVT_MENU, self.OnQuit, id = 113)
self.Bind(wx.EVT_MENU, self.laserlines, id = 114)
self.plotPanel = wxmpl.PlotPanel(self, -1,)
fig = self.plotPanel.get_figure()
self.axes = fig.gca()
self.axes.set_xticks(arange(xmin - 100, xmax + 100, 25))
self.axes.set_yticks(arange(ymin - 100, ymax + 100, 0.1));
self.axes.axis([xmin,xmax,ymin,ymax])
self.axes.grid(True)
self.axes.set_xlabel('Wavelength [nm]',fontsize=16)
self.axes.set_ylabel('Transmission [%] or Intensity
[a.u.]',fontsize=16)
def openex(self, event):
dlg = wx.FileDialog(self, "Choose a Excitation Filter", os.getcwd(),
"", "*.ex*", wx.OPEN)
if dlg.ShowModal() == wx.ID_OK:
pathex = dlg.GetPath()
mypathex = os.path.basename(pathex)
self.SetStatusText("Selected EX: %s" % mypathex)
ex = numpy.loadtxt(pathex)
ex = normspec_filter(ex)
self.gex, = self.axes.plot(ex[:,0],ex[:,1],'b', lw=2,label =
mypathex)
#self.axes.plot(ex[:,0],ex[:,1],'b', lw=2,label = mypathex)
self.axes.axis([xmin,xmax,ymin,ymax])
self.axes.legend(loc=4)
self.plotPanel.draw()
dlg.Destroy()
#...
# --> here is some more code ...
#...
class MyApp(wx.App):
def OnInit(self):
myframe = MyFrame(None, -1, "FilterSpectra")
myframe.CenterOnScreen()
myframe.Show(True)
return True
app = MyApp(0)
app.MainLoop()
--
Dr. Sebastian Rhode
Grünwalder Str. 103a
81547 München
Tel: +49 89 4703091
sebrh...@googlemail.com
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