Hi Aaron,

I have 3 files each containing contigs from newly sequenced genomes of one
bacterial species- 33-130 contigs/genome.  I ran progressive Mauve with
these new sequences against our fully sequenced reference genome.  The
results were not so straightforward (i.e., there should have been much more
alignment) so I am wondering whether Mauve is capable of aligning fragmented
sequences.  Also, I have noticed from my own small scale analyses that some
of the contigs are in reverse orientation relative to our reference.  Could
this be part of the problem?

Thanks so much in advance for your advice.

Best, Ashlee


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