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• Bionet discussion lists will be turned off this week Don Gilbert
• This Bionet discussion list will be turned off in April Don Gilbert
• Bionet at 30 years of open science communication Don Gilbert
• Stable Transfection of NIH-3T3 and 3T3-L1 sushilapthk60
• Imidazole and Amicon concentrators m . golgoljaam
  • Re: Imidazole and Amicon concentrators Wolfgang Schechinger
• Request for dam–/dcm– E. coli Bjorn
  • Re: Request for dam–/dcm– E. coli Björn Johansson
• Re: EGTA kkerns . mobile
  • Re: EGTA Hiranya Roychowdhury
  • Re: EGTA WS
• Re: How to prepare 1M H2O2 using 30% hydrogen peroxide? thekingmbembe
• Trouble with PCR saadlee shehreen
• Stock solutions of spermine/spermidine? rajkumarm574
• short oligonucleotide probe Tm and specificity analysis neda karami
• protein concentration at 280 nm Sudheer Sangeetham
• Re: Drop Dialysis of Ligations shyamals86
• Potassium Phosphate Buffer cvthakor
• Re: Thioester bonds travisschwochert
• gc/ms for sphingosine Ed Siefker
  • Re: gc/ms for sphingosine Wolfgang Schechinger
• Need help on bioinformatics-sequence retrieval Sudheendra Rao N R
• Re: Slimy recombinant E. coli pellet MacGregor, Barbara
• Re: Methods Digest, Vol 137, Issue 1 Virash Gupta
• Vacuolar dye MDY-64: Chemistry? Mode of action? MacGregor, Barbara
• Slimy recombinant e.coli pellet Megha Goyal
  • Re: Slimy recombinant e.coli pellet Wolfgang Schechinger
  • Re: Slimy recombinant e.coli pellet Megha Goyal
  • Re: Slimy recombinant e.coli pellet P J
  • Fwd: Slimy recombinant e.coli pellet Megha Goyal
  • Re: Slimy recombinant e.coli pellet P J
  • Re: Fwd: Slimy recombinant e.coli pellet Megha Goyal
• Stripped and recharged Ni-NTA beads but still getting impurities only with Ni-NTA beads Sudheer Sangeetham
  • RE: Stripped and recharged Ni-NTA beads but still getting impurities only with Ni-NTA beads Phelan, Paul J.
• Phosphate-Buffered Saline precipitate when adding NaOH najib abdellaoui
  • Re: Phosphate-Buffered Saline precipitate when adding NaOH Dmitry Bochkariov
• Need a book "Liposomes: A Practical Approach" Atul Tripathi
• what is the milliQ water pH? Sudheer Sangeetham
  • Re: what is the milliQ water pH? Wolfgang Schechinger
  • Re: what is the milliQ water pH? Dmitry Bochkariov
• pH of urea increasing with increasing concentration Sudheer Sangeetham
• request for pFG and pFL vector map and DNA sequence. Ramachandran.B.
• protein aggregation TieQiao Wu
  • Re: protein aggregation Wolfgang Schechinger
• Biocuration - Portal For Sharing Life Science Information Mohammed Iftekhar
• DNA binding proteins Renato De Souza Pinto Lemgruber
  • Re: DNA binding proteins Bjorn
  • Re: DNA binding proteins heathbowers
• UV 365 nm, Photo crosslinking on ice? Sudheer Sangeetham
  • Re: UV 365 nm, Photo crosslinking on ice? Hiranya Roychowdhury
  • Re: UV 365 nm, Photo crosslinking on ice? Dmitry Bochkariov
• pGreen Lantern assembly by Bjorn MacGregor, Barbara
  • Re: pGreen Lantern assembly by Bjorn Bjorn
• pGreenLantern-1 sequence Bjorn
• pGreen Lantern-1 sequence assembly Bjorn
• pGreen Lantern, possibly helpful MacGregor, Barbara
• Simple PCR to linearize plasmid not working Phelan, Paul J.
• gRNA design and off target selection zhyjiang2006
  • Re: gRNA design and off target selection Vladimir Gainullin
• C1R stable transfection Cano, Enrique
• help: plasmid extraction with unknown contamination Bing Dai
• Re: Air bubbles in my agar plates mincheol . park88
• Phusion 5xbuffer m.kroiher
• skim milk agar Sue Katz
  • Re: skim milk agar askintekin22
• Skim milk agar hints MacGregor, Barbara
• Query about skim milk agar sairahbano47
• 293T cells derivative which needs medium with supplement and would not grow otherwise Alfredo Pagliuca
  • Re: 293T cells derivative which needs medium with supplement and would not grow otherwise Christian Praetorius
• ceq 8000 lpa james jurgenson
  • Re: ceq 8000 lpa chovek69
• pcr Manali Chakraborty
• Request for software OLAIDE YEKEEN
• mitotic spreads preparation pasquale pensieri
• transient expression trouble pasquale pensieri
• Re: pGreen Lantern sequence file jjduke11
  • Re: pGreen Lantern sequence file arregui . leticia
  • Re: pGreen Lantern sequence file Wolfgang Schechinger
  • Re: pGreen Lantern sequence file Bjorn
  • Re: pGreen Lantern sequence file Bjorn
• the absorbance value 1.05 at 260/280 of protein solution Sudheer Sangeetham
  • RE: the absorbance value 1.05 at 260/280 of protein solution Phelan, Paul J.
  • RE: the absorbance value 1.05 at 260/280 of protein solution Nair, Jayakumar
  • Re: the absorbance value 1.05 at 260/280 of protein solution Sudheer Sangeetham
  • Re: the absorbance value 1.05 at 260/280 of protein solution Pow Joshi
• different molecular weights proteins 23 and 66 kDa? Sudheer Sangeetham
  • RE: different molecular weights proteins 23 and 66 kDa? Nair, Jayakumar
  • RE: different molecular weights proteins 23 and 66 kDa? Jayakumar Nair
• Dilute nitric acid for cleaning glassware and lab tools John Dudgeon
  • Re: Dilute nitric acid for cleaning glassware and lab tools Dr S.A.D.P. Senadheera
• Can I estimate the ' Fluorescent proteins' concentration at 280 nm ? Sudheer Sangeetham
• Na2Po2 buffer? Sudheer Sangeetham
  • RE: Na2Po2 buffer? Hans-Georg Koenig
• Re: Cytofluor II (was: "Fluoroscan II software") gettl008
  • Re: Cytofluor II (was: "Fluoroscan II software") kstumpo
• Poly-deoxyribose phosphate? Phelan, Paul J.
• Sequncing the rest of the Gene M Aldeeb
• Miscelle size of detrgent mix Imran khan
  • Re: Miscelle size of detrgent mix Dr Engelbert Buxbaum
• Re: Concentrating protein lysates bimjhanabishwokarma
  • RE: Concentrating protein lysates Deitiker, Philip R
  • Re: Concentrating protein lysates Dr Engelbert Buxbaum
• Re: Kodak MDS 100 digital camera vivek . vasagar
  • Re: Kodak MDS 100 digital camera Sia Ghazvini
• kit recommendation for Baculovirus generation Mike Autry
  • kit recommendation for Baculovirus generation Mike Autry
• lentiviral construct troubleshooting pasquale pensieri
• Glutathione Sepharose chromatography at below-neutral pH Phelan, Paul J.
• Whole cell extract gel samples for western blot Phelan, Paul J.
  • RE: Whole cell extract gel samples for western blot Alejandro Miguel Martin Dunn
  • Re: Whole cell extract gel samples for western blot Fulvio Celsi
  • Re: Whole cell extract gel samples for western blot אנה פלדמן
  • RE: Whole cell extract gel samples for western blot Nair, Jayakumar
  • Re: Whole cell extract gel samples for western blot אנה פלדמן
  • Re: Whole cell extract gel samples for western blot Pow Joshi
• Re: Methods Digest, Vol 114, Issue 2 Virash Gupta
• T4 DNA ligase activity in PCR buffers Bjorn
• Re: Home-Made TA cloning anatomie . zebrafisch
  • RE: Home-Made TA cloning Alejandro Miguel Martin Dunn
  • Re: Home-Made TA cloning Pow Joshi
  • Re: Home-Made TA cloning pasquale pensieri
• co-transfection efficiency pasquale pensieri
  • Re: co-transfection efficiency Imran khan
• About PCR buffer YangJiao
• Methods Digest, Vol 112, Issue 2- change in pH of PBS buffer after dilution virashkgupta
• PBS 1x pH dependence with time Annalisa Neri
  • Re: PBS 1x pH dependence with time Dr Engelbert Buxbaum
  • Re: PBS 1x pH dependence with time Annalisa Neri
• Re: ibmx, dilution julipuli2003
• Re: Methods Digest, Vol 111, Issue 1 Virash Gupta
• Fwd: Request for Binary vectors/Information Kamalaker Nasani
• Storage / dilution Buffer receipe for HRP conjugates? WS
• DNA polymerases free of dNTPs? Tim Fitzwater
• How much time takes to sythesis of one ATP molecule Sudheer Sangeetham
  • Re: How much time takes to sythesis of one ATP molecule Dr Engelbert Buxbaum
• Re: McIlvaine buffer ccdy81
  • Re: McIlvaine buffer WS
• Re: gas chromatography ermanno . faccio
• Re: Methods Digest, Vol 107, Issue 2 Virash Gupta
• Yellow and Orange Colonies on Kan Plates?! WS
  • RE: Yellow and Orange Colonies on Kan Plates?! Hiranya Roychowdhury
  • Re: Yellow and Orange Colonies on Kan Plates?! Christian Praetorius
• pTAQ licence Yannick Lequette
• Re: Methods Digest, Vol 106, Issue 7 Gerchman
• Receipes for homemade TMB reagent (ELISA)? WS
  • E. coli lysis protocol Theresa H
• Protein detection tags that are dependent on proper folding somervillebikes
• Consort E321 Power supply specs & manuals? WS
  • Re: Consort E321 Power supply specs & manuals? Duncan Clark
  • Re: Consort E321 Power supply specs & manuals? WS
• RE: E coli BL21, DE3 Toufic El Arnaout
• Benzoic Acid Assay Justine Mushobozi
  • Re: Benzoic Acid Assay WS
• Publishing Equipment Twaks? WS
  • Re: Publishing Equipment Twaks? Dr Engelbert Buxbaum
• E coli BL21ΔDE3 ? WS
  • RE: E coli BL21?DE3 ? Alejandro Miguel Martin Dunn
• Recipe Qiagen Lysis Buffer FTB (EpiTect Plus FFPE Lysis Kit) Johanna Samuelsson
  • Re: Recipe Qiagen Lysis Buffer FTB (EpiTect Plus FFPE Lysis Kit) WS
• Re: hygromycin phosphotransferase Sidexcited
• SrfI RE Karolina Malec
  • Re: SrfI RE WS
• PCR touch and go June May
  • Re: PCR touch and go Dr Engelbert Buxbaum
  • Re: PCR touch and go Silky
  • Re: PCR touch and go SAID EL MOUATASSIM
• Requesting an interview for Western Blotting Problem RISHABH JAIN
  • Re: Requesting an interview for Western Blotting Problem Dr Engelbert Buxbaum
• micro RNA Gayathri Sindhu Srinivasan
• Re: Heto FD3 freeze dryer manual jvelascoig
• comparative genomics Ed Siefker
• Re: Pharmacia / LKB FPLC System Bus - Any Hints Available? nick
• percoll solution calculations needed divya teja
  • percoll solution calculations needed divya teja
• Re: tRNA cleanup/precipitation? Dmitry Bochkariov
  • Re: tRNA cleanup/precipitation? Nick Theodorakis
  • Re: tRNA cleanup/precipitation? Christian Praetorius
• CBB R250 for microscopy Sudheer Sangeetham
  • RE: CBB R250 for microscopy Hiranya Roychowdhury
  • Re: CBB R250 for microscopy Wye lab
• Re: RT in-situ PCR for detecting plant viruses gdastbau
• Re: looking fo E.coli RY13 tinw1988
  • Re: looking fo E.coli RY13 Dr Engelbert Buxbaum
• Protein conformation stability test with Urea Sudheer Sangeetham
  • Re: Protein conformation stability test with Urea Dr Engelbert Buxbaum
• Increasing Random DNA amplification Deitiker, Philip R
• Methicillin source Duncan Clark
• photocrosslinking Sudheer Sangeetham
• DNA/RNA extraction from mucinous(glycoprotein) samples Ed Siefker
  • Re: DNA/RNA extraction from mucinous(glycoprotein) samples Peter Ellis
  • Re: DNA/RNA extraction from mucinous(glycoprotein) samples WS
• Re: performing statistics on normalized data Michael Sullivan
• Problems extracting DNA with magnetic beads Thomson, Neroli
• Why to use N-lauroylsarcosine for Hybridization? michel saremi
• RNA/Protein UV crosslinking (UVXL) assay Peter Ellis
• Re: Plasmid mix in the clone - the mechanism? Vladimir Gainullin
  • Re: Plasmid mix in the clone - the mechanism? Pow Joshi
  • RE: Plasmid mix in the clone - the mechanism? Dunowska, Magda
  • Re: Plasmid mix in the clone - the mechanism? Jonathan Rupp
• 5x sds sample buffer become brown color while preparing Sudheer Sangeetham

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