On Jan 9, 10:40 pm, Nick Theodorakis <[email protected]> wrote: > On Jan 9, 11:40 am, Jagadish Katam <[email protected]> wrote: > > > Hi, > > > I am working on purifying the DNA by gel extraction procedure and are using > > the commercially available kits for this purpose. A yellow color gel > > solubilization buffer is used to dissolve the sliced gel. > > > Here i would know the composition of this gel solubilization buffer. > > In general, agarose gel solubilization can be done with chaotropic > salts such as NaI, sodium perchlorate, or guanidine HCl. These also > work well in conjunction with silica-based binding resins because DNA > binding to silica is promoted in high salt. I doubt the company will > tell you exactly what is in their buffer, but it is likely similar to > one the salts I mentioned, possibly with additives added as a > stabilizer or other use. > > Nick > > -- > Nick Theodorakis > [email protected] > contact form:http://theodorakis.net/contact.html
Hi, As pointed by Nick this is best done by high molar chaotropic salts. See detailed protocol here: http://books.google.bg/books?id=Mmne6oC8VhIC&pg=PA123&lpg=PA123&dq=agarose+gel+solubilization&source=bl&ots=JhNritZxi_&sig=1GaJXDGphGOToNAYbrXOHjs4j38&hl=bg&ei=ZpVJS_zBHIb-mQOI6NDXDw&sa=X&oi=book_result&ct=result&resnum=3&ved=0CCAQ6AEwAg#v=onepage&q=agarose%20gel%20solubilization&f=false Hope this helps Ivan _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
