Hello everyone!
I will soon try to isolate proteins from melanocytes, I need to get both Triton X-100 soluble and insoluble fractions. For this purpose I will make this lysis buffer; 50 mM Tris, 150 mM NaCl, 1% Triton X-100, 10 mM EDTA, pH 7.2. And for the insoluble fraction I will use the same buffer with 8M urea. So my questions are: 1. Can I autoclave the lysis buffer? Or should I filter it? (22um or 45um) 2. For the lysis buffer with urea, can I also prepare this one before and store it? Or shall I add the urea before use? 3. Can I prepare a urea stock solution? Which concentration and storage conditions would you recommend? Thanks in advance! Iraz Toprak Aydin EPFL SV ISREC, Station 19 Batiment SV, SV 2540 CH-1015 Lausanne Switzerland Tel: +41 21 693 07 36 e-mail: <mailto:[email protected]> [email protected] _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
