-------- Original Message --------
Subject: help with classifiers and assessment of ME greatly appreciated
Date: Tue, 18 Oct 2011 18:57:42 -0400
From: Jordan, Friederike <[email protected]>
To: [email protected] <[email protected]>

Hello everybody,

I am a Master student conducting a morphometric study on New Zealand common dolphin skulls. In addition to manual measurements taken with calipers ee have also aquired 98 landmarks from 20 skulls with a microscribe and I am planning to analyse the shape with MorphoJ. I have read through the help manual of the software, but I am stuck and I was hoping that someone could help me. Sorry, for asking really basic questions, but I have never used the software before and nobody else here has who I could ask for help. We have taken repeated measurements from a number of skulls and I would like to assess the measurement error of each the 98 landmarks for each repeat and. I have trouble uploading classifiers for the files. The names of two repeats are ‘KS09-13Dd_test1’ and ‘KS09-13Dd_test2’. I created a text file with two columns (test: should be the classifier):
ID                              test
KS09-13Dd_test1         test1
KS09-13Dd_test2         test2
First, I created an identifier from this text fiele and afterwards I tried to create the classifiers from the same text file. However, when I upload the file I get an error message saying that the second classifier could not be matched.
Does anybod yhave an idea what I might be oing wrong?

I read that Procrustes ANOVA can be used to assess measurement error, is that correct? However, am I correct that it is not possible to assess the measurement error for each individual landmark in MorphoJ? Does anybody know a software where the individual assessment is possible? If it is possible in morphoJ, do the data points for the 2 skulls (the repeats) have to be combined in a single data set, or should the data points for each skulls be uploaded as separate data sets? When selecting the classifiers in the Procrustes ANOVA window could I just select one classifier for ‘individual’, or do I have to input something for ‘side’ or ‘error1’ and ‘error2’? I tried to carry out a Procrustes ANOVA on one repeat (with the error message of the classifiers) and I got the output below:
Centroid size:
Effect              SS              MS         df        F      P (param.)
Individual       0,000441        0,000441       1

Shape, Procrustes ANOVA:
Effect SS MS df F P (param.) Pillai tr. P (param.)
Individual     0,00001974    0,0000000688      287        NaN         NaN

I was wondering what NaN stands for? I guess the analysis did not work?

Thank you very much in advance!! My email address is : [email protected]

Thanks a lot!
Fidi




Friederike Jordan
MSc Student

Coastal-Marine Research Group
Institute of Natural Sciences
Massey University
Private Bag 102 904
Auckland 0745
New Zealand

Phone: +  ext.41520 Fax: +64 (0) 9 443 9790
Email: [email protected]
Courier address: Massey University, Building 5, Gate 4, Old Albany Highway, Albany, Auckland


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