----- Forwarded message from Rodrigo Lima  -----

     Date: Mon, 28 May 2012 15:48:16 -0400
      From: Rodrigo Lima 
      Reply-To: Rodrigo Lima 
      Subject: standardizing environmental variables
      To: "[email protected]" 

Dear morphometricians,

I'm running a PLS using Procrustes distances in the first block and 
environmental variables, latitude, and longitude in the second block. I'm 
analyzing populations with different numbers of specimens (from 5 to 50 
specimens), and the environmental variables have one value for all individuals 
in each population (e.g. all individuals from population 1 have the following 
values: latitude 50.5, longitude -60.2, temperature 23.7, precipitation 900, 
etc.). I'm doing it in PAST, and I believe it doesn't standardize the variables 
automatically. 

I have a question about the calculation of standardized values:

1 - Should I standardize the environmental variables using all individuals in 
the sample (i.e. if I have 50 individuals in population 1 and 5 individuals in 
population 2, the temperature for population 1 will be represented 50 times and 
the temperature for population 2 will be represented 5 times)? Or should I take 
the value for each population only once (i.e. if I have 15 populations, I'll 
have 15 values for temperature, 15 for precipitation, 15 for latitude, etc.)?

I hope I didn't complicate a simple question... 

Thanks!
Rodrigo

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