----- Forwarded message from Göran Arnqvist -----
Date: Tue, 9 Oct 2012 17:45:52 -0400
From: Göran Arnqvist
Reply-To: Göran Arnqvist
Subject: 3D digital microscopy
To: [email protected]
Dear all,
I wonder if anyone has experience of 3D digital microscopy for
gathering good quality 3D data for geometric morphometrics? It seems
like 3D systems, like the Leica DVM’s or the Hirox KH’s, should be
able to generate reasonably good 3D surface or landmark data? These
systems are based on the principle of focus variation - the limited
depth of field of the optics is utilized to determine depth
information for the specimen. Vertical movement of the specimen
relative to the objective determines the focus information along with
the distance to the optics. They then create 3D images by vertically
stacking the depth of field, focus point to focus point in the depth
gradient created by a motorized stage, followed by 3D tiling. Hirox
claims a Z “resolution” of 0.05 um and a “repeatability” of 0.5 um.
Leica claims “resolution” 0.5 um. However, these numbers refer to the
resolution of the motorized focusing drive and may or may not reflect
the actual resolution/repeatabbility along the Z-dimension in
reconstructed 3D images. I work primarily with insects (or parts
thereof) in the size range 0.1 – 3 mm. Anyone out there who could
share insights and/or experience of these sorts of 3D microscopy
systems?
Cheers,
/Göran Arnqvist
Email: [email protected]
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Prof. Göran Arnqvist
Animal Ecology
Department of Ecology and Evolution
Evolutionary Biology Centre
University of Uppsala
Norbyvägen 18d
SE - 752 36 Uppsala
Sweden
Email: [email protected]
Phone: +46-(0)18-471 2645
Cell phone: +46-(0)70-2935032
Fax: +46-(0)18-471 6484
NEW homepage with PDF downloads and more at:
http://www.anst.uu.se/goarn789/index.html
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----- End forwarded message -----
