Hi everyone,

I'm trying to assess measurement error in a 2D file of 16 individual 
specimens with no object symmetry. For each specimen I have taken two 
different images and then copied each image twice, resulting in four images 
of each specimen. I have prepared ID strings as recommended on the MorphoJ 
online manual (for example phm1_1; phm1_2; phm2_1; phm2_2. The first three 
letters being the individual, the first digit being the image (Error 1) and 
the second digit the replica (Error 2)). Even though I've selected 'Ind', 
Error 1 and Error 2 in the Procrustes Anova text box, when I run it and get 
the results I don't have anything for Error 2. In the Reports tab, it says 
"The effect indicated for Error 2 has no degrees of freedom. The effect was 
dropped from the analysis"

I don't know why Error 2 isn't being calculated and would be very grateful 
for any suggestions or comments. I've appended the output from the Results 
below.

Many thanks,

Alison


Dataset: Humeri

Classifiers used for the Procrustes ANOVA:
Individuals: ind
Error 1: image

Centroid size:
Effect    SS    MS       df        F      P (param.)
Individual   67921.930157     4528.128677      15    22690.43      <.0001
Error 1          3.192978        0.199561      16       3.95      0.0005
Residual         1.618585        0.050581      32

Shape, Procrustes ANOVA:
Effect    SS  MS        df        F      P (param.)   Pillai tr.   P 
(param.)
Individual     0.05386010    0.0000897668      600     123.74      <.0001
Error 1        0.00046428    0.0000007254      640      13.93      <.0001
Residual       0.00006664    0.0000000521     1280




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