Greetings

I have what I think is a simple problem, but I can't figure it out to save my life. I have a script:

#
#  This is written to have the .ncbirc file contain
#
#       [mpiBLAST]
#       Shared=/scratch/uahmam
#       NCBI=/opt/asn/bio/unzipped/genomes
#


setenv data_root H_sapiens/CHR_01
setenv blast_root /opt/asn/apps/mpiBlast_1.4.0/bin

setenv MPI_REQUEST_MAX 128000
setenv MPI_TYPE_MAX 128000

echo "blast_root is "$blast_root
echo "data_root is "$data_root

rm -f -r /scratch/uahmam/H_sapiens
mkdir /scratch/uahmam/H_sapiens
mkdir /scratch/uahmam/H_sapiens/CHR_01

cp /opt/asn/bio/unzipped/genomes/$data_root/hs_ref_chr1.gbk.* /scratch/uahmam/$data_root/.
cp human_v4.fa /scratch/uahmam/$data_root/.
cd /scratch/uahmam/$data_root/
time /usr/bin/mpirun -np 5 $blast_root/mpiblast -p blastn -d $data_root/hs_ref_chr1.gbk -i human_v4.fa -W 7 -F F > blast.output

which yields this output:

============================================================
=====         Summary of your script job               =====
============================================================
  The script file is:  run_blast_Hsol_v_ch1
  The time limit is  40:00:00  HH:MM:SS
  The target directory is:  /home/uahmam
  The memory limit is:  500mb
  The file size limit is:  1gb
  The number of CPUs is:  6
  The job will start running after:  200601101735.07
  Look for:  runblastHsolvc  in queue:  batch

blast_root is /opt/asn/apps/mpiBlast_1.4.0/bin
data_root is H_sapiens/CHR_01
[blastall] WARNING: oligo_id|RefSeq|GB_accession: Could not find index files for database /scratch/uahmam/H_sapiens/CHR_01/H_sapiens/CHR_01/hs_ref_chr1.gbk.001 [blastall] WARNING: oligo_id|RefSeq|GB_accession: Could not find index files for database /scratch/uahmam/H_sapiens/CHR_01/hs_ref_chr1.gbkAOq1u1 [blastall] WARNING: oligo_id|RefSeq|GB_accession: Could not find index files for database /scratch/uahmam/H_sapiens/CHR_01/H_sapiens/CHR_01/hs_ref_chr1.gbk.001 [blastall] WARNING: oligo_id|RefSeq|GB_accession: Could not find index files for database /scratch/uahmam/H_sapiens/CHR_01/hs_ref_chr1.gbkAOq1u1 [blastall] FATAL ERROR: oligo_id|RefSeq|GB_accession: Database /scratch/uahmam/H_sapiens/CHR_01/hs_ref_chr1.gbkAOq1u1 was not found or does not exist
3       1477.8  Bailing out with signal -1
MPI: On host altix3.asc.edu, Program /opt/asn/apps/mpiBlast_1.4.0/bin/mpiblast, Rank 3, Process 18098 called MPI_Abort(<communicator>, 0)

MPI: --------stack traceback-------
sh: line 1: idb: command not found

MPI: -----stack traceback ends-----
MPI: MPI_COMM_WORLD rank 3 has terminated without calling MPI_Finalize()
MPI: aborting job
0.002u 0.014s 24:38.39 0.0%     0+0k 0+0io 124pf+0w

I thought I had the paths set up correctly according to my understanding of the documentation, but something ain't right.

Can anybody offer any ideas where to look?

Thanks

Mike



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