Hi,

To reproduce the problem, would you have data for a single spin
system?  I can reproduce the issue you see with the repository copy of
NESSY using some random data, but it would be useful for debugging
purposes to have a little bit of real data (if you'd like to keep it
completely secret, then you could randomise the data a little).  I
would also highly recommend submitting a bug report for this at
https://gna.org/bugs/?func=additem&group=nessy.  This will help other
NESSY users who encounter the same issue.

Cheers,

Edward




On 26 June 2012 15:19, Susmita Borthakur <[email protected]> wrote:
> Hi Edward,
>
> Thank you for your mail and also for pointing me to the right email address.
> As I mentioned in my previous mail, I tried fitting some R1rho
> (off-resonance) data using NESSY -12.3.1 (on UBUNTU) and received error
> messages. Please see below for the details of the experimental set up and
> the error messages:
>
> Experimental set up-
>
> Experiment Type: R1rho Dispersion (off-resonance)
>
> Heteronuclear Frequency [MHz]:
>
> 81.079
>
> Offset [Hz]:
>
> 2000
>
> Spin-lock time [s]:
>
> 0.02
> 0.04
> 0.06
> 0.08
> 0.1
>
> Spin-lock power [Hz]:
>
> 0.08
>
> Analysis-
>
>
> Execution Protocol......
>
>
> Evaluating Datasets and Experiments:
> ==================================================
>
>
> Checking if project folder is set up...            [Ok]
>
> Evaluating Experiment 1.....
> =============================================
>
>
>  Experiment 1 checked.
>
> -----------------------------------------------------------------
> 1 experiment will be analyzed.
> -----------------------------------------------------------------
>
>
> Experiment mode: Off resonance R1rho relaxation dispersion.
>
> Reading data....done.
>
> Starting R1rho calculation.
>
> Calculating R1rho for residue 1, Offset: 12566.3706Hz
> R1rho: 15.1
> R1rho: 15.1
> R1rho: 15.1
> R1rho: 15.100
> R1rho: -54.45
> R1rho: 8.1449
> R1rho: 8.1449
> R1rho: -0.423
> R1rho: -0.423
> R1rho: -9.194
> R1rho: -9.194
> R1rho: -11.35
> R1rho: -11.35
> R1rho: -11.21
> R1rho: -11.21
> R1rho: -11.21
> R1rho: -11.21
> R1rho: -11.21
> R1rho: -11.21
> R1rho: -11.21
> R1rho: -11.21
> R1rho: -11.21
> R1rho: -11.21
> R1rho: -11.21
>
> Calculating R1rho for residue 2, Offset: 12566.3706Hz
> R1rho: 15.1
> R1rho: 15.1
> R1rho: 15.1
> R1rho: 15.100
> R1rho: -49.77
> R1rho: 8.6127
> R1rho: 8.6127
> R1rho: 0.3406
> R1rho: 0.3406
> R1rho: -8.368
> R1rho: -8.368
> R1rho: -10.65
> R1rho: -10.65
> R1rho: -10.51
> R1rho: -10.51
> R1rho: -10.50
> R1rho: -10.50
> R1rho: -10.50
> R1rho: -10.50
> R1rho: -10.50
> R1rho: -10.50
> R1rho: -10.50
>
> Pooled variance for residue 1, of experiment 1 is 1.0
> Pooled variance for residue 2, of experiment 1 is 1.0
>
> Fitting residue 1 to model 1...
> R1: 0.01/s, R2: 15.1 1/s
> NESSY error> Unhandled exception in thread started by
> NESSY error>
> NESSY error> Traceback (most recent call last):
> NESSY error>   File "/usr/share/nessy/elements/redirect.py", line 93, in
> write
> NESSY error>
> NESSY error> self.report.SetSelection(self.max-self.pageno)
> NESSY error>   File
> "/usr/lib/python2.7/dist-packages/wx-2.8-gtk2-unicode/wx/_controls.py", line
> 3013, in SetSelection
> NESSY error>
> NESSY error> return _controls_.BookCtrlBase_SetSelection(*args, **kwargs)
> NESSY error>   File "/usr/share/nessy/func/calc.py", line 62, in __init__
> NESSY error>
> NESSY error> Run_spinlock(self.main, globalfit=True, onresonance=False)
> NESSY error>   File "/usr/share/nessy/curvefit/calc_R1rho_models.py", line
> 121, in __init__
> NESSY error>
> NESSY error> self.minimise(num_exp=num_exp, model=1, globalfit=globalfit,
> onresonance=onresonance)
> NESSY error>   File "/usr/share/nessy/curvefit/calc_R1rho_models.py", line
> 1013, in minimise
> NESSY error>
> NESSY error> fit = leastsq(fit_func[str(model)], p, args=(y, error, field,
> offset, globalfit, self.main.report_panel, self.main.spec_freq), full_output
> = 1, col_deriv = 1, ftol = self.main.tolerance, xtol =
> self.main.tolerance,maxfev=2000000)
> NESSY error>   File
> "/usr/lib/python2.7/dist-packages/scipy/optimize/minpack.py", line 275, in
> leastsq
> NESSY error>
> NESSY error> raise TypeError('Improper input: N=%s must not exceed M=%s' %
> (n,m))
> NESSY error> TypeError
> NESSY error> :
> NESSY error> Improper input: N=2 must not exceed M=1
> NESSY error>
>
> Any help/suggestions will be highly appreciated.
>
> Best wishes,
> Susmita
>
>
> --
> Susmita Borthakur, D.Phil.
> Postdoctoral Scholar
> Department of Physiology & Biophysics
> School of Medicine
> Case Western Reserve University
> 10900 Euclid Avenue
> Cleveland OH  44106-4970
> tel: +1-216-368-8654
> email: [email protected]
>
>
> _______________________________________________
> Nessy-users mailing list
> [email protected]
> https://mail.gna.org/listinfo/nessy-users
>

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