On Thu, 18 Apr 2019 at 22:32, Viennet, Thibault
<[email protected]> wrote:
>
> ... as well as interleaving (in the sense of indirect dimension increment
> interleaving in a pseudo-3D).
This is single fid interleaving. Together with the temperature
compensation blocks, this should make the experiment self-consistent.
Just check with MeOH or ethylene glycol that the temperate is the same
between the R1, R2 and NOE, as in relaxation you have one of the
coldest NMR experiments (R1) and one of the hottest (R2). The NOE is
also hot. The VT unit response to the 'hot' experiments can cause
quite a disparity between probe head temperature and sample
temperature:
http://www.nmr-relax.com/manual/Temperature_control_and_calibration.html
> However I am a bit confused now by what you mean with "single scan", "single
> fid" and "single experiment" interleaving? Would you comment on this?
I would highly recommend having a read of:
d’Auvergne, E. J. and Gooley, P. R. (2007). Set theory formulation
of the model-free problem and the diffusion seeded model-free
paradigm. Mol. BioSyst., 3(7), 483–494. (
http://dx.doi.org/10.1039/b702202f )
Orekhov, V. Y., Korzhnev, D. M., Diercks, T., Kessler, H., and
Arseniev, A. S. (1999a). 1H-15N NMR dynamic study of an isolated
α-helical peptide (1-36)bacteriorhodopsin reveals the equilibrium
helix-coil transitions. J. Biomol. NMR, 14(4), 345–356. (
http://dx.doi.org/10.1023/a:1008356809071 ).
These two review articles will point you to the extensive literature
on the subject. Both articles have dedicated sections on the subject
of single scan interleaving, but there is a lot more useful
information in there about model-free.
Regards,
Edward
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