I definitely see the point. At 10X the DOF is like, 2-3 µm. Vibrations
of just half of that would cause significant blur.
Jostein
Den 25.09.2017 08:23, skrev David Mann:
On Sep 25, 2017, at 12:21 PM, Mark C <[email protected]> wrote:
Aside from banding the problem with ambient light is that there can be a
surprising amount of movement in subjects due to minor air currents or
vibration.
Many moons ago I visited a lab that produced holograms. They said mushrooms
grow too fast to be imaged without using a high-power pulse laser (which they
didn't have). Speaking across the optical table was avoided due to the
vibrations. It only would have taken a few tens of nanometres to cause
problems.
Cheers,
Dave
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