> >>So it's not clear whether the real limits of what the earth can
>>produce cause the ascetic complex, or whether the ideology comes
>>first, a priori, focusing attention on the limits rather than the
>>possibilities. What ever became of the notion of planning -- figuring
>>out how to accomplish social goals, especially with newer
>>technologies?...
>
>Right new technologies. Let's clone blue-fin tuna.
>
>Louis Proyect
Cuban socialists aren't opposed to genetic engineering per se, though
I don't know if they like eatin' tuna & doubt that they are sanguine
about trends in corporate genetic engineering. :->
***** EJB Electronic Journal of Biotechnology
Papers accepted from next issue of August 15th, 2001
Tilapia chromosomal growth hormone gene expression accelerates growth
in transgenic zebrafish (Danio rerio)
Reynold Morales, Mammalian Cell Genetics Division. Center for Genetic
Engineering and Biotechnology. PO Box 6162, Havana, Cuba.
María Teresa Herrera, Department of Animal and Human Biology. Faculty
of Biology. University of Havana. 25th street No. 455, Havana 10400,
Cuba.
Amílcar Arenal, Center for Genetic Engineering and Biotechnology. PO
Box 387, Camagüey 1, Cuba.
Asterio Cruz, Division of Quality Control and Assurance. Center for
Genetic Engineering and Biotechnology. PO Box 6162, Havana, Cuba.
Oscar Hernández, Center for Genetic Engineering and Biotechnology. PO
Box 387, Camagüey 1, Cuba.
Rafael Pimentel, Center for Genetic Engineering and Biotechnology. PO
Box 387, Camagüey 1, Cuba.
Isabel Guillén, Mammalian Cell Genetics Division. Center for Genetic
Engineering and Biotechnology. PO Box 6162, Havana, Cuba.
Rebeca Martínez, Mammalian Cell Genetics Division. Center for Genetic
Engineering and Biotechnology. PO Box 6162, Havana, Cuba.
Mario P Estrada, Mammalian Cell Genetics Division. Center for Genetic
Engineering and Biotechnology. PO Box 6162, Havana, Cuba.
<http://www.ejb.org/content/next/#> *****
***** EJB Electronic Journal of Biotechnology ISSN: 0717-3458
Vol.1 No.3, Issue of December 15, 1998.
© 1998 by Universidad Católica de Valparaíso -- Chile
INVITED REVIEW ARTICLE
Agrobacterium tumefaciens: a natural tool for plant transformation
Gustavo A. de la Riva*
Laboratory of Environmental Biotechnology. Plant Division. Centre for
Genetic Engineering and Biotechnology (CIGB).
P.O.Box 6162, 10600 Havana, Cuba
Fax: (53-7) 218070, (53-7) 336008
E-mail: [EMAIL PROTECTED]
Joel González-Cabrera
Laboratory of Environmental Biotechnology. Plant Division. Centre for
Genetic Engineering and Biotechnology (CIGB).
P.O.Box 6162, 10600 Havana, Cuba
Fax: (53-7) 218070, (53-7) 336008
E-mail: [EMAIL PROTECTED]
Roberto Vázquez-Padrón
Laboratory of Environmental Biotechnology. Plant Division. Centre for
Genetic Engineering and Biotechnology (CIGB).
P.O.Box 6162, 10600 Havana, Cuba
Fax: (53-7) 218070, (53-7) 336008
E-mail: [EMAIL PROTECTED]
Camilo Ayra-Pardo
Laboratory of Environmental Biotechnology. Plant Division. Centre for
Genetic Engineering and Biotechnology (CIGB).
P.O.Box 6162, 10600 Havana, Cuba
Fax: (53-7) 218070, (53-7) 336008
E-mail: [EMAIL PROTECTED]
*Corresponding author
Keywords: Agrobacterium tumefaciens, Plant transformation, T-DNA
Abstract
Updated information of mechanisms for T-DNA transfer to plant cells
by Agrobacterium tumefaciens is provided, focused on the role played
by the different components of the virulence system. The general
assessments for the establishment of efficient transformation
protocols are discussed with an emphasis in the application of this
methodology to monocotyledonous plants. Based on our own experience,
we present the establishment of sugarcane transformation by A.
tumefaciens as a model of application of this methodology to an
important culture plant species, previously considered recalcitrant
and inaccessible for this type of genetic manipulation.
<http://www.ejb.org/content/vol1/issue3/abstract/1/index.html> *****
See also Tim Wheeler, "Cuba Takes Lead in Genetic Engineering,
Biotechnology," _People's Weekly World_ 14 December 1996 at
<http://www.hartford-hwp.com/archives/43b/176.html>.
Yoshie
