When you use the play function, pyglet creates a new player and loads the 
sound in to it. However, Streaming is enabled by default and streamed 
sounds generally can only be assigned to one player at a time, so you won't 
be able to assign the sound to another player without loading it again or 
purging the original player. To solve this, just disable streaming when you 
load the audio file:

self.soundtrack = pyglet.resource.media('choice.wav',streaming=False)

@self.window.event
def on_key_press(symbol,modifiers):

    self.soundtrack.play()


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