Iv applied the foll eq:

load 1b8e.pdb, A
create B,A
alter_state 1,B,(x,y,z)=(x,-y,40.60-z)

it should form a dimer of my protein but the object "B" is same as my
monomer with  no change... i am unable to find the error..


On Mon, Feb 28, 2011 at 12:39 PM, Tsjerk Wassenaar <tsje...@gmail.com>wrote:

> Hi Kanika,
>
> Let's say your protein is called 'protein'. Then you can obtain the other
> unit corresponding to your second biomt operation as:
>
> create unit2, protein
> alter_state 1,unit2,(y,z)=(-y,40.6-z)
>
> Note this is a shorthand specific to your case. For a full biomt record (R
> t), with R being the rotation matrix (first three columns) and t the
> translation vector (last column), you would have to make the equation
> reflect the matrix operation Rp+t.
>
>
> Hope it helps,
>
>
> Tsjerk
>
>
> On Feb 28, 2011 6:46 AM, "kanika sharma" <ksharma...@gmail.com> wrote:
>
> ok, I know that a dimer can be generated from the BIOMT operationon
>> monomeric unit of protein.
>>
>> I have this protein....but its unclear to me how to apply these
> transformations and what do they represent....
>
> If somebody knows then please let me know...Its really urgent...
>
> REMARK 350 APPLY THE FOLLOWING TO CHAINS: A
>
>
> REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
>       REMARK 350   BIOMT...
> REMARK 350   BIOMT3   2  0.000000  0.000000 -1.000000       40.60000
>
>
>
> Best Regards,
> Kanika
>
>
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