Hi Blaine,

Setting ambient to 0.1 didn't change anything. Setting to 0.4 and higher 
improves brightness but makes representation not very "esthetically pleasing" - 
 the color is definitively oversaturated.

Thanks,
Vitali

-----Original Message-----
From: Mooers, Blaine H.M. (HSC) <blaine-moo...@ouhsc.edu> 
Sent: Tuesday, May 26, 2020 6:31 PM
To: Stanevich, Vitali [JRDUS] <vstan...@its.jnj.com>; 
pymol-users@lists.sourceforge.net
Subject: [EXTERNAL] RE: volume representation is extremely dark colored

WARNING: This email originated from outside the company. Do not click on links 
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Hi Vitali,

Maybe ambient was set to a large negative value. 
Doing so has not impact on line drawings.

Try resetting it to the default value.

set ambient, 0.1

Best regards,

Blaine

Blaine Mooers, Ph.D.
Associate Professor
Department of Biochemistry and Molecular Biology College of Medicine University 
of Oklahoma Health Sciences Center S.L. Young Biomedical Research Center (BRC) 
Rm. 466
975 NE 10th Street, BRC 466
Oklahoma City, OK 73104-5419

________________________________________
From: Stanevich, Vitali [JRDUS] via PyMOL-users 
[pymol-users@lists.sourceforge.net]
Sent: Tuesday, May 26, 2020 4:32 PM
To: pymol-users@lists.sourceforge.net
Subject: [EXTERNAL] [PyMOL] volume representation is extremely dark colored

Hello everyone,

I have a problem of creating useful volume representations in Pymol version 
1.8.6.2<https://urldefense.proofpoint.com/v2/url?u=http-3A__1.8.6.2&d=DwQFAg&c=VjzId-SM5S6aVB_cCGQ0d3uo9UfKByQ3sI6Audoy6dY&r=k0gMbcsdOcdbPUNV5tW66KQSZfXL0ewVDPVBp7tqbks&m=BNWMZg9-_ltMi2O9nPtMsSvn01PANBYBXuDSFdm92O4&s=RPm4waFOpdYkr0DWzikqf33ejGbyEIpp1mroeQkUqjc&e=>.
 Lines representation behaves as expected, but anything volume related (sticks, 
surface, cartoon) is drawn as a very dark surface, see example image at the 
link below:
https://www.dropbox.com/s/nc8vqs42ki5l0zs/vs1.png?dl=0<https://urldefense.proofpoint.com/v2/url?u=https-3A__www.dropbox.com_s_nc8vqs42ki5l0zs_vs1.png-3Fdl-3D0&d=DwMFAg&c=VjzId-SM5S6aVB_cCGQ0d3uo9UfKByQ3sI6Audoy6dY&r=k0gMbcsdOcdbPUNV5tW66KQSZfXL0ewVDPVBp7tqbks&m=BNWMZg9-_ltMi2O9nPtMsSvn01PANBYBXuDSFdm92O4&s=Wug1vVUQk8jevkmDUEfqd8P7wnR8KN82kBpEqNiWbE8&e=>

Is there setting in Pymol which can bring volume-related representation to 
something more normal? Or do you think it's non-Pymol problem with drivers 
compatibility and etc issues?

Thanks in advance!

Vitali Stanevich, PhD
Scientist
Janssen R&D



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