claire morandin wrote: > Thanks Peter, true I did not realize that ercc_contigs is empty, but I am > not sure how to "populate" the dictionary if I only have one column for > the value but no key
You could use a "dummy value" ercc_contigs = {} for line in open('Faq_ERCC_contigs_name.txt'): gene = line.split()[0] ercc_contigs[gene] = None but a better approach is to use a set instead of a dict: ercc_contigs = set() for line in open('Faq_ERCC_contigs_name.txt'): gene = line.split()[0] ercc_contigs.add(gene) -- http://mail.python.org/mailman/listinfo/python-list