Check out mod_python for Apache. Basically, you would write your python app as a server-side script and the end user would interact with your code via a webpage. It's likely that you won't need any GUI code you wrote, as HTML form elements will be your choices in the browser.
Check out PSP: it enables you to mix Python code with an otherwise ordinary HTML page. Mod_Python: http://www.modpython.org/ PSP article: http://www.onlamp.com/pub/a/python/2004/02/26/python_server_pages.html -- http://mail.python.org/mailman/listinfo/python-list