Hello,

I have a data file that I want to run loess on for 36 columns, divide the
original data by the new data, then dividing columns that end in A and B by
those that end in C.  However, I have something wrong in my first step and
am completely stuck on the third.  Could someone help me please?

Here's a snippet of the data file:
Order   Target  GC      AA_001_A        AA_001_B        AA_001_C
1       a       0.584507042     422.94  302.32  412.19
2       b       0.630434783     193.44  182.88  224.96
3       c       0.649350649     132.67  116     136.12
4       d       0.635359116     306.78  203.68  306.98
5       e       0.609271523     276.32  214.73  307.03
6       f       0.626373626     333.93  249.28  421.97
7       g       0.618834081     216.22  200.94  236.27
All columns have 3722 rows.  The columns repeat in that pattern out to
AA_012_C.  

This is the script that I've tried:

gc<-read.delim("AA1_3_GC.txt")
gc2<-gc[,-c(1:2)]
res=cbind()
for(i in colnames(gc[,-1])){
                temp<-loess(i~GC,gc2)
                temp2<-predict(temp)
                if (length(res)==0){
                                res=temp2
                }else{ res=cbind(res,temp2)
                }
}

But I keep getting this error:
Error in model.frame.default(formula = i ~ GC, data = gc2) : 
  variable lengths differ (found for 'GC')

If I manually type in a name, then it works just fine, but obviously I don't
want to do that for 36 columns.  (Or 72 for the next project.)   Where am I
going wrong and how to do I fix this?

For the second step (dividing column after I divide gc2/res), I really am
unsure of where to even start.  I would guess that it would be something
along the lines of 
for(i in colnames(gc[,-1])){
        res[i]/res[i+2]}
But that would only get me A/C, then B/D, etc.  I've spent the last hour
searching for this, but I'm clearly not using the right terms.  Could
someone even point me in the right direction please?  

Any help/suggestions you can give for either/both parts would be really
appreciated.

Thanks,
Rose


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