Hi, I am following the protocol outlined here for analysis of single channel Agilent microarray data:
http://matticklab.com/index.php?title=Single_channel_analysis_of_Agilent_microarray_data_with_Limma I keep getting the following error message when using Limma's read.maimages function to load my data into an RGList object: Error in RG[[a]][, i] <- obj[, columns[[a]]] : number of items to replace is not a multiple of replacement length I think this may be due to my Agilent raw data txt files being in the wrong format. I am having difficulty finding an example Agilent feature extraction raw data txt file online to compare it to. I have attached a screen shot of one of the files I am using below (as the file itself is too large to upload). I would appreciate if someone could let me know if it is in the correct format, and if not then what format it should be in to prevent the above error message from coming up. Thank you, Parisa http://r.789695.n4.nabble.com/file/n4631061/Screen_shot_2012-05-23_at_10.41.56.png Screen_shot_2012-05-23_at_10.41.56.png -- View this message in context: http://r.789695.n4.nabble.com/File-format-for-single-channel-analysis-of-Agilent-microarray-data-in-Limma-tp4631061.html Sent from the R help mailing list archive at Nabble.com. ______________________________________________ R-help@r-project.org mailing list https://stat.ethz.ch/mailman/listinfo/r-help PLEASE do read the posting guide http://www.R-project.org/posting-guide.html and provide commented, minimal, self-contained, reproducible code.
