Dear All, I am trying to perform MANOVA. I have table with 504 columns(species) 
and 36 rows) with two grouping (season and location)
 
Zx <- Z[c(4:504)]
Zxm <- as.matrix(Z)
m<- manova(Zxm~Season*location, data=Z)

when I do summary.aov, I get respond for each species but summary.manova
summary.manova(m) :" residuals have rank" 24<501.

What can it be the reason for this error message?

Thank you,

Ozgul

 Below you can see part of the table.
name    Season  location        Acetobacter     Aerococcus      Alishewanella   
Amaricoccus
xls-nord-01     J       w       0       0,024078979     0       0
bxls-sud-01     J       w       0       0       0       0
brux-nord-04    A       w       0       0       0       0
brux-sud-04     A       w       0       0       0       0
br-nord-07      Ju      w       0       0       0       0
br-sud-07       Ju      w       0       0       0       0
b-nord-10       O       w       0       0       0       0
bsud-10 O       w       0,107836089     0       0,107836089     0,035945363
Z1-01   J       u       0       0       0       0,040567951
Z3-01   J       u       0       0       0       0
Z5-01   J       d       0,023116043     0       0       0
Z7-01   J       d       0,014130281     0       0       0
Z9-01   J       d       0       0       0       0
Z10-01  J       d       0       0       0       0
Z12-01  J       d       0       0       0       0
Z1-04   A       u       0       0       0       0
Z3-04   A       u       0       0       0       0
Z5-04   A       d       0       0       0       0
Z7-04   A       d       0       0       0       0
Z9-04   A       d       0       0,013839873     0       0
Z10-04  A       d       0       0       0       0
Z12-04  A       d       0       0       0       0
Z1-07   Ju      u       0       0       0       0
Z3-07   Ju      u       0       0       0       0
Z5-07   Ju      d       0       0       0       0
Z7-07   Ju      d       0       0       0       0
Z9-07   Ju      d       0       0       0       0
Z10-07  Ju      d       0       0       0       0
Z12-07  Ju      d       0       0,022301517     0       0
Z1-10   O       u       0       0       0       0
Z3-10   O       u       0       0       0       0
Z5-10   O       d       0       0       0       0
Z7-10   O       d       0       0       0,052924054     0
Z9-10   O       d       0       0       0,035050824     0
Z10-10  O       d       0       0       0       0,040783034
Z12-10  O       d       0       0       0       0

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