Hi,
I have an anonymous function called function(x) that will run anova, run 
HSD.test on the model, and then sort the results.  I am passing this anonymous 
function to the "by" function to get results by "Isopair" factor which is my 
index variable.  Since I want to run the anova on multiple dependent variables 
including "Trait1" & "Trait2", I am calling the dependent variable, 
"trait_names" in the model and then passing the "by" function to another 
function called "funC" which takes the "trait_names" as an argument to execute.

After I execute the funC(data_set$trait1), I am getting the results by Isopair 
but the results for the two Isopairs (Isopair-A &Isopair-B) are the same which 
I know is not correct.  It looks like the data is not getting split by Isopairs 
and so ALL data is used in anova for both Isopairs.  Any help in modifying 
function, funC or any other ways to achieve the desired outcome will be highly 
appreciated.

Thanks.  Nilesh

R code, data set, and session info is pasted below.
R code:
library(agricolae)
funC<- function(trait_names){
  by(data_set, data_set$Isopair,function(x){
  mod<- aov(trait_names~ STGgroup*Field + Rep%in%Field, data=data_set)
  out<-HSD.test(mod,"STGgroup",group=TRUE,console=TRUE)
  dfout<- arrange(data.frame(out$groups),desc(trt))
  })
}
Results:
##execute funC function for Trait1 & Trait2
funC(data_set$Trait1)


data_set$Isopair: Isopair-A

      trt    means M

1 STG     776.9167 a

2 Non-STG 779.0833 a

---------------------------------------------------------------------------

data_set$Isopair: Isopair-B

      trt    means M

1 STG     776.9167 a

2 Non-STG 779.0833 a

Data:
data_set<- structure(list(Field = structure(c(1L, 1L, 1L, 1L, 1L, 1L, 1L,
1L, 1L, 1L, 1L, 1L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L, 2L,
2L), .Label = c("LML6", "TZL2"), class = "factor"), Isopair = structure(c(1L,
1L, 1L, 1L, 1L, 1L, 2L, 2L, 2L, 2L, 2L, 2L, 1L, 1L, 1L, 1L, 1L,
1L, 2L, 2L, 2L, 2L, 2L, 2L), .Label = c("Isopair-A", "Isopair-B"
), class = "factor"), STGgroup = structure(c(1L, 1L, 1L, 2L,
2L, 2L, 1L, 1L, 1L, 2L, 2L, 2L, 1L, 1L, 1L, 2L, 2L, 2L, 1L, 1L,
1L, 2L, 2L, 2L), .Label = c("Non-STG", "STG"), class = "factor"),
    Rep = structure(c(1L, 2L, 3L, 1L, 2L, 3L, 1L, 2L, 3L, 1L,
    2L, 3L, 1L, 2L, 3L, 1L, 2L, 3L, 1L, 2L, 3L, 1L, 2L, 3L), .Label = c("Rep1",
    "Rep2", "Rep3"), class = "factor"), Trait1 = c(686L, 641L,
    642L, 727L, 619L, 562L, 808L, 739L, 744L, 873L, 797L, 868L,
    782L, 783L, 675L, 713L, 762L, 641L, 1009L, 995L, 845L, 1186L,
    912L, 663L), Trait2 = c(45L, 65L, 70L, 35L, 20L, 80L, 70L,
    65L, 70L, 20L, 30L, 35L, 40L, 55L, 35L, 40L, 35L, 40L, 40L,
    35L, 25L, 40L, 35L, 25L)), .Names = c("Field", "Isopair",
"STGgroup", "Rep", "Trait1", "Trait2"), class = "data.frame", row.names = c(NA,
-24L))

Session info:

R version 3.1.3 (2015-03-09)

Platform: i386-w64-mingw32/i386 (32-bit)

Running under: Windows 7 x64 (build 7601) Service Pack 1



locale:

[1] LC_COLLATE=English_United States.1252  LC_CTYPE=English_United States.1252

[3] LC_MONETARY=English_United States.1252 LC_NUMERIC=C

[5] LC_TIME=English_United States.1252



attached base packages:

[1] grid      stats     graphics  grDevices utils     datasets  methods   base



other attached packages:

 [1] gridExtra_0.9.1 Hmisc_3.16-0    Formula_1.2-1   survival_2.38-1 
caret_6.0-41    ggplot2_1.0.1

 [7] lattice_0.20-30 MASS_7.3-39     dplyr_0.4.1     agricolae_1.2-1



loaded via a namespace (and not attached):

 [1] acepack_1.3-3.3     assertthat_0.1      boot_1.3-15         
BradleyTerry2_1.0-6

 [5] brglm_0.5-9         car_2.0-25          cluster_2.0.1       coda_0.17-1

 [9] codetools_0.2-10    colorspace_1.2-6    combinat_0.0-8      DBI_0.3.1

[13] deldir_0.1-9        digest_0.6.8        foreach_1.4.2       foreign_0.8-63

[17] gtable_0.1.2        gtools_3.4.1        iterators_1.0.7     klaR_0.6-12

[21] latticeExtra_0.6-26 lazyeval_0.1.10     LearnBayes_2.15     lme4_1.1-7

[25] magrittr_1.5        Matrix_1.1-5        mgcv_1.8-4          minqa_1.2.4

[29] munsell_0.4.2       nlme_3.1-120        nloptr_1.0.4        nnet_7.3-9

[33] parallel_3.1.3      pbkrtest_0.4-2      plyr_1.8.1          proto_0.3-10

[37] quantreg_5.11       RColorBrewer_1.1-2  Rcpp_0.11.6         reshape2_1.4.1

[41] rpart_4.1-9         scales_0.2.4        sp_1.0-17           SparseM_1.6

[45] spdep_0.5-88        splines_3.1.3       stringr_0.6.2       tools_3.1.3





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