Hi  Matt
It looks like you are trying to obtain the upstream sequence data information given transcription factor binding sites???

There are a lot of functions in Bioconductor which are optimized for this type of analysis. If you wish to either post on the Bioconductor mailing list (https://support.bioconductor.org/), or tell me a little more, I can perhaps help.

Also see http://bioconductor.org/help/search/index.html?q=transcription+factor+binding/. There are several workflows and lots of docs on TFBS analysis.

Also Matt as you are local to Boston, I am happy to point you to local Boston resources if you are new to R/Bioconductor.

Best
Aedin

--
Aedin Culhane
---------------
Department of Biostatistics and Computational Biology,Dana-Farber Cancer 
Institute
Department of Biostatistics, Harvard TH Chan School of Public Health
email: ae...@jimmy.harvard.edu

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