Hi Salvatore, Depending upon your concept of "size" the use of the weighted sum may well suit your purpose. The first principal component, being three lengths and a mass, is likely to be strongly related to any sensible concept of "size". My comment was meant to ensure that the local definition of "size" was what you wanted. Using scaled values is a good idea as it provides an intuitive measure of comparison within the population. Remember that if your animal is long and thin, you have already reduced the importance of the former measurement by scaling.
Jim On Mon, Nov 14, 2016 at 12:37 PM, Sidoti, Salvatore A. <sidoti...@buckeyemail.osu.edu> wrote: > Hi Jim, > > Nice to see you again! First of all, apologies to all for bending the rules a > bit with respect to the mailing list. I know this is a list for R programming > specifically, and I have received some great advice in this regard in the > past. I just thought this was an interesting applied problem that would > generate some discussion about PCA in R. > > Yes, that is an excellent question! Indeed, why not just volume? Since this > is still a work in progress and we have not published as of yet, I would > rather not be more specific about the type of animal at this time ;>}. > Nonetheless, I can say that the animals I study change "size" depending on > their feeding and hydration state. The abdomen in particular undergoes > drastic size changes. That being said, there are key anatomical features that > remain fixed in the adult. > > Now, there *might* be a way to work volume into the PCA. Although volume is > not a reliable metric since the abdomen size is so changeable while the > animal is alive, but what about preserved specimens? I have many that have > been marinating in ethanol for months. Wouldn't the tissues have equilibrated > by now? Probably... I could measure volume by displacement or suspension, I > suppose. > > In the meantime, here's a few thoughts: > > 1) Use the contribution % (known as C% hereafter) of each variable on > principle components 1 and 2. > > 2) The total contribution of a variable that explains the variations > retained by PC1 an PC2 is calculated by: > > sum(C%1 * eigenvalue1, C%2 * eigenvalue2) > > 3) Scale() to mean-center the columns of the data set. > > 4) Use these total contributions as the weights of an arithmetic mean. > > For example, we have an animal with the following data (mean-centered): > weight: 1.334 > interoc: -0.225 > clength: 0.046 > cwidth: -0.847 > > The contributions of these variables on PC1 and PC2 are (% changed to > proportions): > weight: 0.556 > interoc: 0.357 > clength: 0.493 > cwidth: 0.291 > > To calculate size: > 1.334(0.556) - 0.225(0.357) + 0.046(0.493) - 0.847(0.291) = 0.43758 > Then divide by the sum of the weights: > 0.43758 / 1.697 = 0.257855 = "animal size" > > This value can then be used to rank the animal according to its size for > further analysis... > > Does this sound like a reasonable application of my PCA data? > > Salvatore A. Sidoti > PhD Student > Behavioral Ecology > > -----Original Message----- > From: Jim Lemon [mailto:drjimle...@gmail.com] > Sent: Sunday, November 13, 2016 3:53 PM > To: Sidoti, Salvatore A. <sidoti...@buckeyemail.osu.edu>; r-help mailing list > <r-help@r-project.org> > Subject: Re: [R] Principle Component Analysis: Ranking Animal Size Based On > Combined Metrics > > Hi Salvatore, > If by "size" you mean volume, why not directly measure the volume of your > animals? They appear to be fairly small. Sometimes working out what the > critical value actually means can inform the way to measure it. > > Jim > > > On Sun, Nov 13, 2016 at 4:46 PM, Sidoti, Salvatore A. > <sidoti...@buckeyemail.osu.edu> wrote: >> Let's say I perform 4 measurements on an animal: three are linear >> measurements in millimeters and the fourth is its weight in milligrams. So, >> we have a data set with mixed units. >> >> Based on these four correlated measurements, I would like to obtain one >> "score" or value that describes an individual animal's size. I considered >> simply taking the geometric mean of these 4 measurements, and that would >> give me a "score" - larger values would be for larger animals, etc. >> >> However, this assumes that all 4 of these measurements contribute equally to >> an animal's size. Of course, more than likely this is not the case. I then >> performed a PCA to discover how much influence each variable had on the >> overall data set. I was hoping to use this analysis to refine my original >> approach. >> >> I honestly do not know how to apply the information from the PCA to this >> particular problem... >> >> I do know, however, that principle components 1 and 2 capture enough of the >> variation to reduce the number of dimensions down to 2 (see analysis below >> with the original data set). >> >> Note: animal weights were ln() transformed to increase correlation with the >> 3 other variables. >> >> df <- data.frame( >> weight = log(1000*c(0.0980, 0.0622, 0.0600, 0.1098, 0.0538, 0.0701, >> 0.1138, 0.0540, 0.0629, 0.0930, >> 0.0443, 0.1115, 0.1157, 0.0734, 0.0616, 0.0640, 0.0480, 0.1339, >> 0.0547, 0.0844, >> 0.0431, 0.0472, 0.0752, 0.0604, 0.0713, 0.0658, 0.0538, 0.0585, >> 0.0645, 0.0529, >> 0.0448, 0.0574, 0.0577, 0.0514, 0.0758, 0.0424, 0.0997, 0.0758, >> 0.0649, 0.0465, >> 0.0748, 0.0540, 0.0819, 0.0732, 0.0725, 0.0730, 0.0777, 0.0630, >> 0.0466)), >> interoc = c(0.853, 0.865, 0.811, 0.840, 0.783, 0.868, 0.818, 0.847, 0.838, >> 0.799, >> 0.737, 0.788, 0.731, 0.777, 0.863, 0.877, 0.814, 0.926, 0.767, >> 0.746, >> 0.700, 0.768, 0.807, 0.753, 0.809, 0.788, 0.750, 0.815, 0.757, >> 0.737, >> 0.759, 0.863, 0.747, 0.838, 0.790, 0.676, 0.857, 0.728, 0.743, >> 0.870, >> 0.787, 0.773, 0.829, 0.785, 0.746, 0.834, 0.829, 0.750, 0.842), >> cwidth = c(3.152, 3.046, 3.139, 3.181, 3.023, 3.452, 2.803, 3.050, 3.160, >> 3.186, >> 2.801, 2.862, 3.183, 2.770, 3.207, 3.188, 2.969, 3.033, 2.972, >> 3.291, >> 2.772, 2.875, 2.978, 3.094, 2.956, 2.966, 2.896, 3.149, 2.813, >> 2.935, >> 2.839, 3.152, 2.984, 3.037, 2.888, 2.723, 3.342, 2.562, 2.827, >> 2.909, >> 3.093, 2.990, 3.097, 2.751, 2.877, 2.901, 2.895, 2.721, 2.942), >> clength = c(3.889, 3.733, 3.762, 4.059, 3.911, 3.822, 3.768, 3.814, 3.721, >> 3.794, >> 3.483, 3.863, 3.856, 3.457, 3.996, 3.876, 3.642, 3.978, 3.534, >> 3.967, >> 3.429, 3.518, 3.766, 3.755, 3.706, 3.785, 3.607, 3.922, 3.453, >> 3.589, >> 3.508, 3.861, 3.706, 3.593, 3.570, 3.341, 3.916, 3.336, 3.504, >> 3.688, >> 3.735, 3.724, 3.860, 3.405, 3.493, 3.586, 3.545, 3.443, >> 3.640)) >> >> pca_morpho <- princomp(df, cor = TRUE) >> >> summary(pca_morpho) >> >> Importance of components: >> Comp.1 Comp.2 >> Comp.3 Comp.4 >> Standard deviation 1.604107 0.8827323 0.7061206 >> 0.3860275 >> Proportion of Variance 0.643290 0.1948041 0.1246516 >> 0.0372543 >> Cumulative Proportion 0.643290 0.8380941 0.9627457 >> 1.0000000 >> >> Loadings: >> Comp.1 Comp.2 Comp.3 Comp.4 >> weight -0.371 0.907 -0.201 >> interoc -0.486 -0.227 -0.840 >> cwidth -0.537 -0.349 0.466 -0.611 >> clength -0.582 0.278 0.761 >> >> Comp.1 Comp.2 Comp.3 Comp.4 >> SS loadings 1.00 1.00 1.00 1.00 >> Proportion Var 0.25 0.25 0.25 0.25 >> Cumulative Var 0.25 0.50 0.75 1.00 >> >> Any guidance will be greatly appreciated! >> >> Salvatore A. Sidoti >> PhD Student >> The Ohio State University >> Behavioral Ecology >> >> ______________________________________________ >> R-help@r-project.org mailing list -- To UNSUBSCRIBE and more, see >> https://stat.ethz.ch/mailman/listinfo/r-help >> PLEASE do read the posting guide >> http://www.R-project.org/posting-guide.html >> and provide commented, minimal, self-contained, reproducible code. ______________________________________________ R-help@r-project.org mailing list -- To UNSUBSCRIBE and more, see https://stat.ethz.ch/mailman/listinfo/r-help PLEASE do read the posting guide http://www.R-project.org/posting-guide.html and provide commented, minimal, self-contained, reproducible code.