Your line of code:
zzz.aov <- aov(Intensity ~ Group + Error(Sample), data = zzzanova) indicates that you are trying to do a repeated measures ANOVA, not just an ANOVA. The Error(Sample) term in your expression indicates that Sample is a within subjects factor, which I presume is not the case. The way I understood your data, the Sample column is just an id number for the subjects - is that right? If you just want to compare the means of Intensity for the the four Groups, then just do: zzz.aov <- aov(Intensity ~ Group, data = zzzanova) You may want to consult this resource for explicit examples of ANOVA in R: http://www.personality-project.org/R/r.anova.html Ravi Kulkarni -- View this message in context: http://n4.nabble.com/Help-with-ANOVA-in-R-tp1585992p1586936.html Sent from the R help mailing list archive at Nabble.com. ______________________________________________ R-help@r-project.org mailing list https://stat.ethz.ch/mailman/listinfo/r-help PLEASE do read the posting guide http://www.R-project.org/posting-guide.html and provide commented, minimal, self-contained, reproducible code.