Hello sir: As to the "variance stabilization" method which is applied in the "vsn"package under R environment,here's a question about the ratio of the 2 channels: After applying vsn,we can get new_cy5 and new_cy3 intensity according to the original cy5 and cy3 intensity respectively. And the new_cy5 new_cy3 are similar with ln(intensity).
But how can I calculate the ratio=cy5/cy3 ? If the new_cy5 and new_cy3 is log2(intensity),the ratio equals to 2^(log2cy5-log2cy3),but as to vsn,how to get the ratio? Since the vsn transformation is similar to ln transformation when the intensity is high, I wanna know how high the intensity is so that I can use ln instead of vsn? Thanks a lot! my best regards! ______________________________________________ [EMAIL PROTECTED] mailing list https://stat.ethz.ch/mailman/listinfo/r-help PLEASE do read the posting guide! http://www.R-project.org/posting-guide.html
