Hi all,

I am trying to use PERMANOVA in a one factor design to test for differences in diet among species (species are in rows and foods in columns).I however have different sample sizes for each species (groups).

In Anderson, 2001 I found that the PERMANOVA method is for balanced designs but it could be modified for unbalanced designs.Does adonis account for differences in sample size among groups?I couldn’t find direct reference to this in the Vegan manual or tutorial.I read that MRPP can be used for unbalanced designs. I'm not sure about Anosim.I would greatly appreciate any suggestions.

Thanks,

Bibiana
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