Dear Gavin,

Thank you very much. I tried with your advice but the result is almost
unchanged...

> metaMDS(data_matrix2, distance="bray", k=2, autotransform=F, trymax=100,
halfchange=F) -> metaMDS_new
Run 0 stress 0.05172131
Run 1 stress 0.04877468
.....
Run 47 stress 0.04663743
Run 48 stress 0.05066009
Run 49 stress 0.04525287
... New best solution
... procrustes: rmse 0.001156733  max resid 0.002525323
*** Solution reached

> metaMDS_new

Call:
metaMDS(comm = data_matrix2, distance = "bray", k = 2, trymax = 100,
autotransform = F, halfchange = F)

global Multidimensional Scaling using monoMDS

Data:     data_matrix2
Distance: bray

Dimensions: 2
Stress:     0.04525287
Stress type 1, weak ties
Two convergent solutions found after 49 tries
Scaling: centring, PC rotation
Species: expanded scores based on ‘data_matrix2’

Any further advice??


Gian




2012/2/20 Gavin Simpson <gavin.simp...@ucl.ac.uk>

> On Mon, 2012-02-20 at 16:28 +0100, Gian Maria Niccolò Benucci wrote:
> > Hi r-sig-ecology Members,
> >
> > I would please you to examine the differences I found in metaMDS() and
> > nmds() outputs and possibly draw some conclusions. As I wrote in past
> > emails I have 24 samples of ectomycorrhizal fungi grouped into two
> > different communities (12 natural and 12 cultivated).
> >
> > here is the code:
> >
> > > metaMDS(data_matrix2, distance="bray", k=2, autotransform=F) -> metaMDS
> > > metaMDS
> >
> > Call:
> > metaMDS(comm = data_matrix2, distance = "bray", k = 2, autotransform = F)
> >
> > global Multidimensional Scaling using monoMDS
> >
> > Data:     data_matrix2
> > Distance: bray
> >
> > Dimensions: 2
> > *Stress:     0.04536661 *
> > Stress type 1, weak ties
> > No convergent solutions - best solution after 20 tries
>
> Perhaps you could try running metaMDS with random starts until it does
> find convergent solutions?! Why expect metaMDS() to do better in 20
> tries than nmds() with 100 random tries?
>
> add `trymax = 100` and `halfchange = FALSE` to try to make the two
> functions more comparable.
>
> HTH
>
> G
>
> > Scaling: centring, PC rotation, halfchange scaling
> > Species: expanded scores based on data_matrix2
> >
> > > nmds <- nmds(dist_bray, mindim = 2, maxdim = 2, nits = 100)
> > Using random start configuration
> > Using random start configuration
> > ...
> > > nmds_min
> >             X1          X2
> > 1  -0.66226262  0.16392824
> > 2 -0.68844987 -0.20891993
> > 3 -0.47717515  0.55323693
> > 4 -0.67213392 -0.09195847
> > 5 0.03305172  0.41844232
> > 6   0.31992006  0.53887910
> > 7  -0.61944875  0.34411146
> > 8 0.07013849  0.65723057
> > 9 0.53053436  0.37444633
> > 10  0.26704705  0.39849341
> > 11 -0.20981416  0.70391983
> > 12 -0.61620355 -0.43568743
> > 13 0.14219660 -0.69571194
> > 14 0.42365981 -0.43308767
> > 15 0.15840330 -0.12872863
> > 16 -0.00492407 -0.06889680
> > 17 -0.20513513 -0.58052156
> > 18 0.46546214  0.12019775
> > 19 0.36753709 -0.25048256
> > 20 -0.09446486 -0.61943616
> > 21  0.15010553 -0.31995009
> > 22  0.41540872 -0.29507200
> > 23  0.42573367 -0.12321584
> > 24  0.48081354 -0.02121688
> > > min(nmds$stress)
> > [*1] 0.2787161*
> > > nmds$r2[which.min(nmds$stress)]
> > [1] 0.6338372
> >
> > Is it possible I got so different stress values??
> >
> > Thanks for replying,
> >
> > _______________________________________________
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>  Dr. Gavin Simpson             [t] +44 (0)20 7679 0522
>  ECRC, UCL Geography,          [f] +44 (0)20 7679 0565
>  Pearson Building,             [e] gavin.simpsonATNOSPAMucl.ac.uk
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