Kate — others can give you more in-depth information, but I believe (i.e., my 
students and I and colleagues believe it) NMDS does indeed use pairwise 
distance measures, in place of  eigenvector calculations, in computing 
ordination scores.  Some of these distance-based measures, like the Sorensen’s 
distance, are not true “metrics”, in that they do not obey the triangle 
inequality; hence “non-metric” scaling, but still fully quantitative with the 
scores being continuous variables.  As such they can be used as response 
variables in OLS and other regression-type models.

Others may correct me if I misspoke.  

As you probably know, there has been considerable heat generated in the 
ecological community over the relative value of distance-based vs. eigenvector 
methods for ordination.  My sense from the debate is that when your community 
data are presence-absence the distance-based measures are more robust, but you 
will hear arguments against that too.

> On Jul 16, 2015, at 12:19 PM, Kate Boersma <kateboer...@gmail.com> wrote:
> 
> Hi all.
> 
> I have a methodological question regarding non-metric multidimensional 
> scaling. This is not specific to R. Feel free to refer me to another 
> venue/resource if there is one more appropriate to my question.
> 
> Correct me if I'm wrong: NMDS axes are non-metric, which is why NMDS 
> frequently makes sense for community data, but it also means that distances 
> in NMDS ordination space cannot be interpreted simplistically as they can in 
> eigenvalue-based methods like PCA. This is why it is inadvisable 
> (meaningless) to use NMDS axes as response variables in a linear modeling 
> framework (e.g., with environmental variables as predictors).
> 
> My question is this: Does that mean that it is also inadvisable to use 
> distances among points in ordination space as response variables?
> 
> My (potentially flawed) understanding: While the coordinates may not make 
> sense in isolation, they should be meaningful relative to each other. In a 2D 
> ordination, if communities A & B are closer together in ordination space than 
> communities C & D, that means they have more similar species compositions. 
> Therefore, I should be able to predict the distance between points in a 
> linear modeling framework.
> 
> Alternately, I could use the actual distances among communities from my 
> dissimilarity matrix with a method like db-RDA. But I used NMDS over RDA or 
> CCA for a reason. It seems more straightforward to use the distances from my 
> NMDS ordination instead of generating new coordinates from a PCoA to fit an 
> RDA framework (as in db-RDA)... but this logic only works if NMDS distances 
> are informative.
> 
> Are these comparable analyses? If not, why not?
> 
> I'd love your opinions.
> 
> Thank you,
> Kate
> 
> -- 
> Kate Boersma, PhD
> Department of Biology
> University of San Diego
> 5998 Alcala Park
> San Diego CA 92110
> kateboer...@gmail.com
> http://www.oregonstate.edu/~boersmak/
> 
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