Dear all: I have a few questions regarding the use of PERMANOVA (adonis function in vegan) and maybe someone here might be able to help.
I want to use the adonis function to compare abundance data from 6 locations at different times. I have 35 samples for each location at each time, totalling 805 observations. My independent variables are Location (nested in Habitat type) and Campaign (nested in Season). Now the questions are: 1 - regarding my replicates (the 35 samples for each location/time): they enter the analysis or I must calculate average them and use this average on the Permanova? If they enter the analysis (i.e. no average is needed) to I need to specify it as a factor in strata? 2 - Nested factors: I'm using a formula like this: adonis(Myabund_matrix ~ Habitat/Location * Season/Campaign, strata=Location, data=Mydata_matrix) Is this the correct approach? Do I need anything else in strata? Thanks in advance for your attention. All the best: Pedro Neves [[alternative HTML version deleted]] _______________________________________________ R-sig-ecology mailing list R-sig-ecology@r-project.org https://stat.ethz.ch/mailman/listinfo/r-sig-ecology