Alastair,

That's indeed the correct work-around. The is because a different code is used whether the NEXUS file has a TRANSLATE block or not. I'll fix the bug very soon.

Cheers,

Emmanuel

PS: thanks for the tip on using Figtree.

Alastair Potts wrote on 24/02/2011 14:57:
Hi All,
I think I have located the problem. As usual I had experienced it before and I found (or someone else found) a workaround - but I couldn't remember it.

The bootstrap values ($node.label) is mixed up when I save the bootstrap tree as a nexus file with a translation table in PAUP (this is the default by the way). If I save the bootstrap tree without a translation table (i.e. FORMAT=ALTNEX), then the BS values are in their correct places.

If I open both saved bootstrap trees (i.e. with and without translation tables) in FigTree, I get identical trees with the bootstrap labels in the correct places. Obviously this has not been the case in R. I think that there may be a problem when reading in the translation table when there are values included with each node. I've included examples of both saved files from PAUP if anyone wishes to play around with the problem.

So, for those of you using PAUP, the quick work-around to avoid this problem in future is to use SAVETREES ... FORMAT=ALTNEX ...

Cheers,
Alastair



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Alastair Potts
PhD candidate
Botany Department
University of Cape Town
alastair.po...@uct.ac.za or pott...@gmail.com
University Private Bag, Rondebosch 7700, South Africa
or
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Emmanuel Paradis wrote:
Hi Alastair,

I copied the Newick string below into R, read it with read.tree, and this seems to work as normal.

I also tried to do the same with Figtree: the tree and its tip labels are OK, but the node labels are not (eg, it displays "377" for the root instead of "100"). My guess is that it's a problem when decoding the strings (maybe specific to Linux).

Best,

Emmanuel

Alastair Potts wrote on 23/02/2011 21:09:
Hi All,
I'm sure I've had this problem before and found a way to fix it. I've spent an hour looking for the solution, but can't seem to find it.

In short, I've run a bootstrap analysis in PAUP* which provided a consensus tree with bootstrap values. This tree opens fine in FigTree. I read it in using ape's read.nexus and plot the node using nodelabel and the $node.label property of the imported tree. I've done nothing else to the tree (e.g. ladderize which I know sometimes causes problems), yet my node label bootstrap values are all shuffled around in some way and I can't figure out why.

My code is simply:

pt <- read.nexus('dataset1.bootstrap.swap.NNI.tree')
plot(pt,cex=0.7)
nodelabels(pt$node.label)

Possibly the problem lies with the nexus tree, so here it is for what it's worth: tree PAUP_1 = [&U] (1:0,((2:100,(3:100,4:100)80:80,5:100,6:100,(7:100,10:100)60:60,8:100,9:100,(11:100,34:100)60:60,15:100,(16:100,37:100,54:100,56:100,57:100,71:100,72:100)70:70,18:100,(23:100,25:100,30:100,31:100,35:100,116:100,117:100)59:59,26:100,27:100,32:100,33:100,39:100,48:100,58:100,59:100,60:100,64:100,69:100,70:100,78:100,79:100,80:100,82:100,(83:100,84:100,85:100)52:52,94:100,99:100,101:100,102:100,107:100,119:100)70:70,(12:100,(13:100,14:100,53:100,90:100)69:69,28:100,(62:100,63:100)96:96,(66:100,67:100)69:69,68:100,91:100,92:100,93:100,97:100,98:100,100:100)81:81,(17:100,19:100,21:100,24:100,38:100,42:100,43:100,(44:100,45:100)67:67,47:100,49:100,50:100,61:100)82:82,(20:100,40:100,41:100,51:100,52:100,73:100,74:100,108:100,109:100,110:100,118:100)65:65,(22:100,29:100,36:100,46:100,75:100,76:100,105:100,111:100,112:100,113:100,114:100,115:100)97:97,55:100,(65:100,86:100,89:100,96:100)68:68,(77:100,(81:100,95:100,103:100,104:100)68:68,106:100)65:65,(87:100,88:100)98:98)100:100)1
00

;


My apologies for not setting up a standard replication of the error - but I'm not really sure how to go about it with this example.

Thanks in advance for your help.

Cheers,
Alastair

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Emmanuel Paradis
IRD, Jakarta, Indonesia
http://ape.mpl.ird.fr/

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