Hi Carlos.

If you like to use for loops, you could do something like:

analysis<-list()
p.value<-vector()
for (i in 1:5 ) {
  analysis[[i]] <- aov.phylo(x~y,trees[[i]], nsim=100)
  p.value[i]<-attr(analysis[i], "summary")$ "Pr(phy)"[1]
}

All the best, Liam

Liam J. Revell, Assistant Professor of Biology
University of Massachusetts Boston
web: http://faculty.umb.edu/liam.revell/
email: liam.rev...@umb.edu
blog: http://blog.phytools.org

On 3/17/2015 3:58 PM, Carlos H. Biagolini Junior wrote:
  Thanks Liam, it was very helpful!

Now, I am able to run the analysis by your way. However, now arises
another question:

Looking the “multiPhylo” structure, I tried to run the analyses in a
looping format, in this way:

analysis <- NULL


for (i in 1:100 ) {

   analysis[i] <- aov.phylo(x~y,trees[[(i)]], nsim=100)

}


The p values are presented in console, but I did not found a way to
bring this into an object (below one of my attempts). I tried to recover
the p value inside de loop, but when the loop ends, the stored p values
are all the same (probably the p value of the last run of the loop).


for (i in 1:5 ) {

   analysis[i] <- aov.phylo(x~y,trees[[(i)]], nsim=100)

   p.value[i]<-attr(analysis, "summary")$ "Pr(phy)"[1]

}


Thanks again for your help,

All the best,

-         - Carlos


On Tue, Mar 17, 2015 at 10:55 AM, Liam J. Revell <liam.rev...@umb.edu
<mailto:liam.rev...@umb.edu>> wrote:

    Hi Carlos.

    If you read in a set of trees they will be stored as an object of
    class "multiPhylo" which is a list of trees in memory.

    To iterate aov.phylo across all the trees in this object (let's say,
    trees), you could do the following (modifying variable names as
    appropriate):

    fits<-lapply(trees,aov.phylo,__formula=y~x,nsim=100)

    Then to pull out the 'phylogenetic p-values' only in a vector you
    could do:

    Pr.phy<-sapply(fits,function(__x) attr(x,"summary")$"Pr(phy)"[1]__)

    All the best, Liam

    Liam J. Revell, Assistant Professor of Biology
    University of Massachusetts Boston
    web: http://faculty.umb.edu/liam.__revell/
    <http://faculty.umb.edu/liam.revell/>
    email: liam.rev...@umb.edu <mailto:liam.rev...@umb.edu>
    blog: http://blog.phytools.org

    On 3/16/2015 5:29 PM, Carlos H. Biagolini Junior wrote:

        Hello, I would like to ask for an advice:

        I am using the function aov.phylo () in Geiger package (Garland,
        1993), to
        run a phylogenetic ANOVA.  I have had collected data in
        literature, and I
        am using trees from the birdtree.org <http://birdtree.org> web
        site (for bird phylogenies).

        My difficulty is: How can I perform a sequence of ANOVAs using
        my dataset
        and the list of phylogenetic trees.

        I aim to get each tree inside this list, and write a looping to
        run the
        phylogenetic ANOVA, and store the results (i.e. p value) in an
        object.
        However, I did not realize how can I systematically capture each
        tree in
        this list (since the name of the trees follows a random number
        sequence,
        i.e., within the file has trees with name: tree_3937, tree_2141,
        tree_8734,
        etc...).


        Thanks


        -          Carlos Biagolini



        Following my script (for now):



        library(geiger)

        setwd("D:/...")



        # Introducing the trees

        all.trees<-read.nexus("__mytreefile.tre") # input the 100 trees

        single.tree<- all.trees$ tree_3937 # one tree inside the list



        # Creating a simulated dataset for exemplify

        matrix<- matrix(rnorm (65, mean = 0.5, sd = 0.1),nrow=65,ncol=1)

        species<- single.tree$tip.label # Carrying all species names for
        an object

        rownames(matrix)<-species # Naming each data

        group <-as.factor(rep(c("A","B","C",__"D","E"),each=13)) ;

        d1 <- matrix[,1] ;

        names(group) <- rownames(matrix);



        # Phylogenetical ANOVA

        aov.phylo(d1~group, single.tree, nsim=1000)






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--
Carlos Biagolini-Jr
Universidade Federal de São Carlos
http://lattes.cnpq.br/4086237188108947

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