Dear Klaus,

You were correct.  This fixed the problem.

Many thanks,

Mike

On Fri, Jan 1, 2016 at 5:41 PM, Klaus Schliep <klaus.schl...@gmail.com>
wrote:

> Happy New Year,
> it seems you have not properly installed the packages and you may want
> also update R.
> Cheers, Klaus
>
> On Fri, Jan 1, 2016 at 12:34 AM, Michael Pauers <mjpau...@gmail.com>
> wrote:
>
>> Dear list,
>>
>> First, Happy New Year!
>>
>> Second, I will apologize in advance, as I am nothing more than a duffer
>> with R, so I may ask several follow-up questions to this one.
>>
>> I have a set of 1000 trees that lack branch lengths.  I have used
>> previously used ape to calculate branch lengths on a single tree, but
>> never
>> on a multiphylo object.  I did find and have tried the approach documented
>> at this link:
>>
>> http://blog.phytools.org/2015/04/phylogenetic-regression-when-branch.html
>>
>> but I received a few error messages, including:
>>
>> > library(phytools)
>> Error in gzfile(file, "rb") : cannot open the connection
>> In addition: Warning messages:
>> 1: package ‘phytools’ was built under R version 3.0.3
>> 2: In read.dcf(file.path(p, "DESCRIPTION"), c("Package", "Version")) :
>>   cannot open compressed file 'C:/Program
>> Files/R/R-3.0.2/library/ape/DESCRIPTION', probable reason 'No such file or
>> directory'
>> 3: In gzfile(file, "rb") :
>>   cannot open compressed file '', probable reason 'No such file or
>> directory'
>> > library(phangorn)
>> Error in gzfile(file, "rb") : cannot open the connection
>> In addition: Warning messages:
>> 1: package ‘phangorn’ was built under R version 3.0.3
>> 2: In read.dcf(file.path(p, "DESCRIPTION"), c("Package", "Version")) :
>>   cannot open compressed file 'C:/Program
>> Files/R/R-3.0.2/library/ape/DESCRIPTION', probable reason 'No such file or
>> directory'
>> 3: In gzfile(file, "rb") :
>>   cannot open compressed file '', probable reason 'No such file or
>> directory'
>>
>> and:
>>
>> > library(ape)
>> > ## simulate 200 pure-birth trees:
>> > trees<-ttrees<-pbtree(n=50,scale=1,nsim=200)
>> Error: could not find function "pbtree"
>> > ## ttrees contains the trees with their original branch lengths
>> > ## we'll perform manipulations on trees
>> > foo<-function(tree){
>> +     obj<-fastBM(tree,nsim=2)
>> +     colnames(obj)<-c("x","y")
>> +     as.data.frame(obj)
>> + }
>> > xy<-lapply(ttrees,foo)
>> Error in lapply(ttrees, foo) : object 'ttrees' not found
>> > library(nlme)
>> > fit.model<-function(tree,data){
>> +     data$v<-diag(vcv.phylo(tree))
>> +     fit<-gls(y~x,data=data,correlation=corBrownian(1,tree),
>> +         weights=varFixed(~v))
>> +     setNames(c(coefficients(fit)[2],anova(fit)$"p-value"[2]),
>> +         c("beta","p-value"))
>> + }
>> > fit.true<-t(mapply(fit.model,trees,xy))
>> Error in mapply(fit.model, trees, xy) : object 'xy' not found
>> > mean(fit.true[,1]) ## should be zero
>> Error in mean(fit.true[, 1]) : object 'fit.true' not found
>> > noogtree<-lapply(noogtree,compute.brlen)
>> > class(noogtree)<-"multiPhylo"
>> > fit.grafen<-t(mapply(fit.model,noogtree,xy))
>> Error in mapply(fit.model, noogtree, xy) : object 'xy' not found
>> > utils:::menuInstallPkgs()
>> Error in install.packages(NULL, .libPaths()[1L], dependencies = NA, type =
>> type) :
>>   no packages were specified
>> > fit.grafen<-t(mapply(fit.model,noogtree))
>> Error in data$v <- diag(vcv.phylo(tree)) :
>>   argument "data" is missing, with no default
>> > fit.grafen<-t(mapply(fit.model,noogtree,xy))
>> Error in mapply(fit.model, noogtree, xy) : object 'xy' not found
>> > mean(fit.grafen[,1])
>> Error in mean(fit.grafen[, 1]) : object 'fit.grafen' not found
>> > foo<-function(tree){
>> +     tree$edge.length<-rep(1,nrow(tree$edge))
>> +     tree
>> + }
>> > trees<-lapply(ttrees,foo)
>> Error in lapply(ttrees, foo) : object 'ttrees' not found
>> > class(trees)<-"multiPhylo"
>> > fit.equal<-t(mapply(fit.model,trees,xy))
>> Error in mapply(fit.model, trees, xy) : object 'xy' not found
>> > mean(fit.equal[,1]) ## should be zero
>> Error in mean(fit.equal[, 1]) : object 'fit.equal' not found
>>
>> Does anyone have any advice?  Please let me know if I should provide more
>> information.
>>
>> Thanks in advance,
>>
>> Mike Pauers
>>
>>         [[alternative HTML version deleted]]
>>
>> _______________________________________________
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>
>
>
>
> --
> Klaus Schliep
> Postdoctoral Fellow
> Revell Lab, University of Massachusetts Boston
>
>

        [[alternative HTML version deleted]]

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