It might be easier to use ace() in ape. A major advantage is it gives you the 
uncertainty in the estimates, which is often substantial (and even so still an 
underestimate, since there could be trends that aren't fit at all).

library(ape)
data(bird.orders)
x <- rnorm(23)
reconstruction <- ace(x, bird.orders)

and then you have the states at each node (with the node numbers):

print(reconstruction$ace)

and the confidence intervals:

print(reconstruction$CI95)

There are various ways to get node numbers for the node you care about; perhaps 
the easiest is by specifying a pair of tips:

ape::getMRCA(bird.orders, c("Struthioniformes", "Tinamiformes"))

which will tell you the relevant node number of the reconstructions (the label 
for the vector, not the index (i.e., use reconstruction$ace["26"] with the 
quotes).

Hope this helps (and love to phytools in general, even if not the tool for 
this),
Brian


From: R-sig-phylo <[email protected]> on behalf of Jaime Adrian 
Cordova via R-sig-phylo <[email protected]>
Date: Thursday, November 20, 2025 at 6:00 PM
To: [email protected] <[email protected]>
Subject: [R-sig-phylo] Phytools connect ace values to tree

Hi colleagues,

I've conducted an ancestral state reconstruction for E.coli growth rates under 
anaerobic conditions using phytools. I'd like to get a bit more detail out of 
reconstruction, particularly with the ancestral values; however, I'm not 
exactly sure how to match the values to the nodes in the phylogeny. In other 
words, I'm able to map the values onto the map using contMap(), but I would 
like to be able to say that the MRCA of a phylogroup is predicted to have a 
certain growth rate, etc.

Can someone help me with this? Thanks!

Kind regards,
Jaime

Jaime A. Cordova Jr.
Pronouns: he/him/his/�l
Genetics PhD Candidate, Perna Lab
University of Wisconsin, Madison
NASA/JPL Solar System Ambassador

I tend to work at irregular hours, but I never expect others to do the same. If 
I email you outside of your working hours, please do not feel obligated to 
respond until you are comfortable doing so.

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