Hi, This can be tricky. Can you estimate the percentage of monomer verses dimer? Can you also estimate the amount of time the peptide binds to the receptor? Do you have binding data for the NMR sample conditions? If the more than 90% of the receptor is bound to the peptide and the time bound to the receptor is significant, then you should be able to handle this with standard model-free analysis using a single diffusion tensor. This will likely require data at 2 or more field strengths. Seb, have you worked with this situation?
Regards, Edward On 23 February 2011 11:04, Maddy Strickland <m.strickl...@bristol.ac.uk> wrote: > Hi everyone, > > I'm moving on to looking at dynamics of binding to a receptor which I've > already studied using Relax for Lipari-Szabo data. I was wondering if I > added some unlabelled peptide to the 15n receptor how I could study the > new relaxation data? Can I still use Relax and the same pulse sequences > etc. or do I need a program specific to binding? It's a fairly strong > binder, but it would render the receptor more of a cylinder than a > sphere... What do you all think? > > > Madeleine Strickland > > > > _______________________________________________ > relax (http://nmr-relax.com) > > This is the relax-users mailing list > relax-users@gna.org > > To unsubscribe from this list, get a password > reminder, or change your subscription options, > visit the list information page at > https://mail.gna.org/listinfo/relax-users > _______________________________________________ relax (http://nmr-relax.com) This is the relax-users mailing list relax-users@gna.org To unsubscribe from this list, get a password reminder, or change your subscription options, visit the list information page at https://mail.gna.org/listinfo/relax-users
