Dear Samtools Support,
I am studying mitochondrial DNA and wanted to include heteroplasmic
variants in my list of variants. The third and fourth numbers of the I16
field denote the number of total non-reference variants. However, I was
wondering if there was a reliable measure of the number of each individual
variant? I've noticed that the QS fields is a useful estimate of frequency
of each variant, but from another thread on the mailing list, it was
mentioned that the QS fields are unreliable.
Best,
Min Seong Kim
Cornell University
undergraduate researcher
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