[Samtools-help] how to generate mpileup output for indels similar to pileup output for indels

[Ma, Qicheng]

Hi ,

      Could anyone help me how to generate mpileup output for indels similar to 
old pileup output for indels ?

      Here is the example, there is single base deletion at position 360.

     In the old pileup output attached here, there is one more line:

WT      359     *       -a/-a   23789   303081  59      7917    -a      * 7505  
  412     0       0       0

    which the new mpileup output attached here does not have.


    The old pileup was generated by:

$samtools/samtools-0.1.8/samtools  pileup  -cf IST03663_S4_L001.fa    
IST03663_S4.bwa.mem.bam.sort.bam  > 
IST03663_S4_consensus_pileup_w_indels_0.1.8.txt

    The new mpileup was generated by:

$samtools/current/samtools mpileup  -A --min-MQ 5   --min-BQ 30  -d 800000  -f 
IST03663_S4_L001.fa  IST03663_S4.bwa.mem.bam.sort.bam
-o  IST03663_S4_consensus_pileup_w_indels.txt


Thanks,
                          Best regards, Mit freundlichen Grüssen, Meilleures 
salutations,  此致 敬礼

Qicheng Ma<http://publicationslist.org/qicheng.ma>, Ph. D.
Investigator III
Novartis Institutes for BioMedical
Research, Inc.
220 Massachusetts Avenue
Cambridge, MA 02139
USA

Phone    +1  617 8717218
Fax        +1  617 8715783
qicheng...@novartis.com<mailto:qicheng...@novartis.com>
http://www.nibr.com/research/discovery/pathways.shtml


WT      359     c       C       255     0       59      7917    
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WT      359     c       7696    
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WT      360     a       0               

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