Could it be that readlines() leaves the \n at the end?
try with 

self.agents = [a.strip() for a in f.readlines()]

or similar

On Friday, March 21, 2014 12:09:11 PM UTC+1, James Ford wrote:
>
> Sure,
>
> Below you will find a crawl of http://doc.scrapy.org with a depth of 1 
> and extraction of inlinks only.
>
> http://pastebin.com/wE292pQe
>
> As you can see from the stats the status 200 count is only 13. This is not 
> the case if I put my agent-list directly in my module or if I disable my 
> middleware.
>
> Thanks
>
> Den fredagen den 21:e mars 2014 kl. 10:55:26 UTC+1 skrev Paul Tremberth:
>>
>> Can you share logs? 
>>
>> On Fri, Mar 21, 2014 at 10:53 AM, James Ford <[email protected]> wrote: 
>> > Hello, 
>> > 
>> > I'm having an odd issue with one of my projects. 
>> > 
>> > I have implemented a custom middleware that rotates user-agent for each 
>> > request. 
>> > 
>> > The middleware works by reading from a file when the middleware is 
>> > initialized by putting the contents of the file into a list(in memory). 
>> > 
>> > According to me this should work fine, but I am getting a large amount 
>> of 
>> > 400 bad requsts of my crawls? The odd thing is that it works fine if I 
>> just 
>> > put the agents in a list directly instead of reading from file. 
>> > 
>> > What can cause this error? Here is my middleware: 
>> > 
>> > class UserAgentPool(): 
>> >     def __init__(self): 
>> >         basepath = os.path.dirname(__file__) 
>> >         filepath = os.path.abspath(os.path.join(basepath, 
>> "agents.txt")) 
>> >         with open(filepath, 'r') as f: 
>> >             self.agents = f.readlines() 
>> > 
>> >     def rotate(self): 
>> >         log.msg("Rotating user agent", level=log.DEBUG) 
>> >         agent = self.agents.pop(0) 
>> >         log.msg("Agent popped %s" %agent, level=log.DEBUG) 
>> >         log.msg("[%s]" % ", ".join(map(str, self.agents)), 
>> level=log.DEBUG) 
>> >         self.agents.append(agent) 
>> >         return agent 
>> > 
>> > class UserAgentRotationMiddleware(object): 
>> >     def __init__(self): 
>> >         self.pool = UserAgentPool() 
>> > 
>> >     def process_request(self, request, spider): 
>> >         if getattr(spider, 'agent_rotation', None): 
>> >             agent = self.pool.rotate() 
>> >             request.headers.setdefault('User-Agent', agent) 
>> >             log.msg("Setting User-Agent to %s" 
>> > %request.headers["User-Agent"]) 
>> > 
>> > 
>> > -- 
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>>
>

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