Re: [ccp4bb] Rhodamine in FPLC
30% acetic acid solution should wash it out (exercise some care, it's quite a strong vinegar). Hypochlorite and peroxide will bleach the stain, but technically speaking the leftover oxidized species will still be stuck in there, just colorless. Artem www.harkerbio.com "making lots of *really tiny* drops... for fun and profit" - Cosmic Cats approve of this message On Fri, Jun 9, 2017 at 6:21 PM, Tomas Malinauskas < tomas.malinaus...@gmail.com> wrote: > Dear Reza, > I used hydrogen peroxide (~0.1-2%?) to remove rhodamine stuck on > Superdex columns. "Pink" columns turned "white" after this kind of > treatment. > Hope that helps, > Tomas > > On Fri, Jun 9, 2017 at 10:43 PM, Reza Khayat > wrote: > > Hi, > > > > Sorry for the non crystallography question. We're using Rhodamine for > labeling some material that we need to run through our FPLC. It seems that > the Rhodamine is getting stuck to the PEEK tubing. Anyone has experience > with successfully removing the Rhodamine from the tubing? Thanks. > > > > Best wishes, > > Reza > > > > Reza Khayat, PhD > > Assistant Professor > > City College of New York > > Department of Chemistry > > New York, NY 10031 >
Re: [ccp4bb] Rhodamine in FPLC
Dear Reza, I used hydrogen peroxide (~0.1-2%?) to remove rhodamine stuck on Superdex columns. "Pink" columns turned "white" after this kind of treatment. Hope that helps, Tomas On Fri, Jun 9, 2017 at 10:43 PM, Reza Khayat wrote: > Hi, > > Sorry for the non crystallography question. We're using Rhodamine for > labeling some material that we need to run through our FPLC. It seems that > the Rhodamine is getting stuck to the PEEK tubing. Anyone has experience with > successfully removing the Rhodamine from the tubing? Thanks. > > Best wishes, > Reza > > Reza Khayat, PhD > Assistant Professor > City College of New York > Department of Chemistry > New York, NY 10031
[ccp4bb] Rhodamine in FPLC
Hi, Sorry for the non crystallography question. We're using Rhodamine for labeling some material that we need to run through our FPLC. It seems that the Rhodamine is getting stuck to the PEEK tubing. Anyone has experience with successfully removing the Rhodamine from the tubing? Thanks. Best wishes, Reza Reza Khayat, PhD Assistant Professor City College of New York Department of Chemistry New York, NY 10031