[galaxy-user] Deleting a Galaxy User

2011-11-10 Thread Oren Livne

Dear All,
Is it possible to delete a galaxy user or multiple users with a UI call 
or an API call?

Thanks,
Oren
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[galaxy-user] Question about building a reference genome index using Bowtie

2011-11-10 Thread William Light
I am trying to compare two genetically different strains that I have
sequenced using SOLiD.  I was trying to ask where these two strains are
different, either in terms of deletions or polymorphisms, and one idea I
had was to use Bowtie to create an index from one of these strains and then
to map my reads for the other strain using this index.  Is this a
reasonable strategy?

 - Will Light
   Northwestern University
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[galaxy-user] Genetrack-Indexer/PeakPredictor

2011-11-10 Thread Stefanie Ververs

Hi everybody,

I'm using the Genetrack-Peak-Predictor to predict nucleosome positions.
I still have some questions:

1) Am I correct that the genetrack indexer seems to be down on the 
Public Galaxy Server? I get a server error, when I start it.
(The Genetrack Browser doesn't work either; although I'm still not quite 
sure whether there are dependencies.)


2) By now, I know one paper on Genetrack - 
http://bioinformatics.oxfordjournals.org/content/24/10/1305.short and I 
found the following presentation slides: 
http://ged.msu.edu/angus/tutorials-2011/files/lecture-chipseq.pdf. 
Galaxy tells me to "cite Blankenberg D, et al. In preparation."


Is there additional information? It would be great to know how exactly 
the peak predictor works, but the slides give only a kind of overview, 
but of course no explaining and no details and the paper isn't that clear.


Thanks,

Steffi/
///
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Re: [galaxy-user] Genetrack-Indexer/PeakPredictor

2011-11-10 Thread Daniel Blankenberg
Hi Steffi,

GeneTrack should be working again on the Main server, thanks for reporting the 
error.

For information on the inner workings of GeneTrack, you should consult the 
paper that you mentioned, along with http://genetrack.bx.psu.edu/ and you can 
additionally contact the GeneTrack author, who I've CC'd here.


Thanks for using Galaxy,

Dan


On Nov 10, 2011, at 11:32 AM, Stefanie Ververs wrote:

> Hi everybody,
> 
> I'm using the Genetrack-Peak-Predictor to predict nucleosome positions.
> I still have some questions:
> 
> 1) Am I correct that the genetrack indexer seems to be down on the Public 
> Galaxy Server? I get a server error, when I start it.
> (The Genetrack Browser doesn't work either; although I'm still not quite sure 
> whether there are dependencies.)
> 
> 2) By now, I know one paper on Genetrack - 
> http://bioinformatics.oxfordjournals.org/content/24/10/1305.short and I found 
> the following presentation slides: 
> http://ged.msu.edu/angus/tutorials-2011/files/lecture-chipseq.pdf. Galaxy 
> tells me to "cite Blankenberg D, et al. In preparation."
> 
> Is there additional information? It would be great to know how exactly the 
> peak predictor works, but the slides give only a kind of overview, but of 
> course no explaining and no details and the paper isn't that clear.
> 
> Thanks,
> 
> Steffi
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> 
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> please use the interface at:
> 
>  http://lists.bx.psu.edu/

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Re: [galaxy-user] Deleting a Galaxy User

2011-11-10 Thread Jennifer Jackson

Hi Oren,

I am posting the answer given to the galaxy-dev mailing list below for 
other users, even thought this is a development topic. Next time, please 
only post to a single mailing list.


Thanks!

Jen
Galaxy team

Repost:

On Nov 10, 2011, at 11:41 AM, Oren Livne wrote:

> Dear Nate,
>
> Thanks for your answer.
>
> 1. I could not find it in the admin panel in the UI. When I click on 
"Manage users", I get the user list and can select users, but there is 
no "Delete User" button.

Hi Oren,

Hrm, it looks like the option to delete users was removed from the 
interface since it couldn't be made conditional on a configuration 
setting (due to a limitation in Galaxy's "Grids" feature, which is used 
to display the table of users in the UI).  They can be added by 
uncommented lines 98-100 in lib/galaxy/web/controllers/admin.py:


#operations.append( grids.GridOperation( "Delete", condition=( 
lambda item: not item.deleted ), allow_multiple=True ) )
#operations.append( grids.GridOperation( "Undelete", condition=( 
lambda item: item.deleted and not item.purged ), allow_multiple=True ) )
#operations.append( grids.GridOperation( "Purge", condition=( 
lambda item: item.deleted and not item.purged ), allow_multiple=True ) )


> 2. Which call in the code deletes a user? I can then write my own API 
method.
mark_user_deleted() in lib/galaxy/web/base/controller.py.  The preferred 
method for implementing API methods is by moving the code in the web UI 
controller which performs the actual action to lib/galaxy/actions/ and 
then changing the API and UI methods to call that method.  See the 
implementation of the Quota API for a reference.


> 3. If I try to reset passwords for more than one user at a time, I 
get the error trackback:

>
> TypeError: Odd-length string URL: 
http://128.135.107.157:8080/admin/reset_user_password
> Module weberror.evalexception.middleware:364 in respond 
 view

>>>  app_iter = self.application(environ, detect_start_response)
> Module paste.debug.prints:98 in __call__  view
>>>  environ, self.app)
> Module paste.wsgilib:539 in intercept_output  view
>>>  app_iter = application(environ, replacement_start_response)
> Module paste.recursive:80 in __call__  view
>>>  return self.application(environ, start_response)
> Module paste.httpexceptions:632 in __call__  view
>>>  return self.application(environ, start_response)
> Module galaxy.web.framework.base:160 in __call__  view
>>>  body = method( trans, **kwargs )
> Module galaxy.web.framework:173 in decorator  view
>>>  return func( self, trans, *args, **kwargs )
> Module galaxy.web.base.controller:2025 in reset_user_password 
 view

>>>  user = get_user( trans, user_id )
> Module galaxy.web.base.controller:2413 in get_user  
 view

>>>  id = trans.security.decode_id( id )
> Module galaxy.web.security:45 in decode_id  view
>>>  return int( self.id_cipher.decrypt( obj_id.decode( 'hex' ) 
).lstrip( "!" ) )

> Module encodings.hex_codec:42 in hex_decode  view
>>>  output = binascii.a2b_hex(input)
> TypeError: Odd-length string
>
> 4. A similar problem occurs when I try to delete multiple data 
libraries under "Manage data libraries". One library deletion works, but 
multiple yields an error message:

>
> Invalid Library id ( 
df7a1f0c02a5b08e,0a248a1f62a0cc04,03501d7626bd192f,3f5830403180d620,2a56795cad3c7db3,417e33144b294c21,5a1cff6882ddb5b2,2d9035b3fc152403,4b187121143038ff,c9468fdb6dc5c5f1,1e8ab44153008be8,e85a3be143d5905b 
) specifield.
Thanks for reporting these, we'll fix them as soon as possible.  I've 
created a bitbucket here to track them:


https://bitbucket.org/galaxy/galaxy-central/issue/678/some-user-and-library-grid-operations

--nate



On 11/9/11 3:14 PM, Oren Livne wrote:

Dear All,
Is it possible to delete a galaxy user or multiple users with a UI call
or an API call?
Thanks,
Oren
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--
Jennifer Jackson
http://usegalaxy.org
http://galaxyproject.org/wiki/Support
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Re: [galaxy-user] Genetrack-Indexer/PeakPredictor

2011-11-10 Thread Daniel Blankenberg

> I think in general you should make sure to cite the authors of the
> original publication in addition to Galaxy, the note in Galaxy should
> make this explicit.

Absolutely agree, the citations are arguably the most important part.  The 
GeneTrack paper citation is currently listed in the help for the tools at 
http://usegalaxy.org and in -central. Any place where a citation is missing is 
an error and will be corrected as soon as it is reported.


Thanks for using Galaxy,

Dan

On Nov 10, 2011, at 12:55 PM, Istvan Albert wrote:

> Hello Everyone,
> 
>> Galaxy tells me to "cite Blankenberg D, et al. In preparation."
> 
> I think in general you should make sure to cite the authors of the
> original publication in addition to Galaxy, the note in Galaxy should
> make this explicit.
> 
> Small tidbits that may be useful. There is a command line version for
> genetrack  with its source code at:
> 
> https://github.com/ialbert/chipexo
> 
> this is a fork of my son's project while he rotated in a lab, he
> ported a number of tools including GeneTrack to a command line
> interface. Seems to work well but I have not ran it for large genomes.
> 
> In the course that I teach the lectures covering ChipSeq analysis: 19,
> 20 and 21 cover the usage and principles of GeneTrack (among other
> topics)
> 
> http://bcc.bx.psu.edu/courses/597D-2011/index-597D-2011.html
> 
> best regards,
> 
> Istvan
> 
> 
> On Thu, Nov 10, 2011 at 12:19 PM, Daniel Blankenberg  wrote:
>> Hi Steffi,
>> GeneTrack should be working again on the Main server, thanks for reporting
>> the error.
>> For information on the inner workings of GeneTrack, you should consult the
>> paper that you mentioned, along with http://genetrack.bx.psu.edu/ and you
>> can additionally contact the GeneTrack author, who I've CC'd here.
>> 
>> Thanks for using Galaxy,
>> Dan
>> 
>> On Nov 10, 2011, at 11:32 AM, Stefanie Ververs wrote:
>> 
>> Hi everybody,
>> 
>> I'm using the Genetrack-Peak-Predictor to predict nucleosome positions.
>> I still have some questions:
>> 
>> 1) Am I correct that the genetrack indexer seems to be down on the Public
>> Galaxy Server? I get a server error, when I start it.
>> (The Genetrack Browser doesn't work either; although I'm still not quite
>> sure whether there are dependencies.)
>> 
>> 2) By now, I know one paper on Genetrack -
>> http://bioinformatics.oxfordjournals.org/content/24/10/1305.short and I
>> found the following presentation slides:
>> http://ged.msu.edu/angus/tutorials-2011/files/lecture-chipseq.pdf. Galaxy
>> tells me to "cite Blankenberg D, et al. In preparation."
>> 
>> Is there additional information? It would be great to know how exactly the
>> peak predictor works, but the slides give only a kind of overview, but of
>> course no explaining and no details and the paper isn't that clear.
>> 
>> Thanks,
>> 
>> Steffi
>> ___
>> The Galaxy User list should be used for the discussion of
>> Galaxy analysis and other features on the public server
>> at usegalaxy.org.  Please keep all replies on the list by
>> using "reply all" in your mail client.  For discussion of
>> local Galaxy instances and the Galaxy source code, please
>> use the Galaxy Development list:
>> 
>>  http://lists.bx.psu.edu/listinfo/galaxy-dev
>> 
>> To manage your subscriptions to this and other Galaxy lists,
>> please use the interface at:
>> 
>>  http://lists.bx.psu.edu/
>> 
> 
> 
> 
> -- 
> Istvan Albert
> Associate Professor, Bioinformatics
> Pennsylvania State University
> http://www.personal.psu.edu/iua1/
> 


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Re: [galaxy-user] Genetrack-Indexer/PeakPredictor

2011-11-10 Thread Istvan Albert
Hello Everyone,

> Galaxy tells me to "cite Blankenberg D, et al. In preparation."

I think in general you should make sure to cite the authors of the
original publication in addition to Galaxy, the note in Galaxy should
make this explicit.

Small tidbits that may be useful. There is a command line version for
genetrack  with its source code at:

https://github.com/ialbert/chipexo

this is a fork of my son's project while he rotated in a lab, he
ported a number of tools including GeneTrack to a command line
interface. Seems to work well but I have not ran it for large genomes.

In the course that I teach the lectures covering ChipSeq analysis: 19,
20 and 21 cover the usage and principles of GeneTrack (among other
topics)

http://bcc.bx.psu.edu/courses/597D-2011/index-597D-2011.html

best regards,

Istvan


On Thu, Nov 10, 2011 at 12:19 PM, Daniel Blankenberg  wrote:
> Hi Steffi,
> GeneTrack should be working again on the Main server, thanks for reporting
> the error.
> For information on the inner workings of GeneTrack, you should consult the
> paper that you mentioned, along with http://genetrack.bx.psu.edu/ and you
> can additionally contact the GeneTrack author, who I've CC'd here.
>
> Thanks for using Galaxy,
> Dan
>
> On Nov 10, 2011, at 11:32 AM, Stefanie Ververs wrote:
>
> Hi everybody,
>
> I'm using the Genetrack-Peak-Predictor to predict nucleosome positions.
> I still have some questions:
>
> 1) Am I correct that the genetrack indexer seems to be down on the Public
> Galaxy Server? I get a server error, when I start it.
> (The Genetrack Browser doesn't work either; although I'm still not quite
> sure whether there are dependencies.)
>
> 2) By now, I know one paper on Genetrack -
> http://bioinformatics.oxfordjournals.org/content/24/10/1305.short and I
> found the following presentation slides:
> http://ged.msu.edu/angus/tutorials-2011/files/lecture-chipseq.pdf. Galaxy
> tells me to "cite Blankenberg D, et al. In preparation."
>
> Is there additional information? It would be great to know how exactly the
> peak predictor works, but the slides give only a kind of overview, but of
> course no explaining and no details and the paper isn't that clear.
>
> Thanks,
>
> Steffi
> ___
> The Galaxy User list should be used for the discussion of
> Galaxy analysis and other features on the public server
> at usegalaxy.org.  Please keep all replies on the list by
> using "reply all" in your mail client.  For discussion of
> local Galaxy instances and the Galaxy source code, please
> use the Galaxy Development list:
>
>  http://lists.bx.psu.edu/listinfo/galaxy-dev
>
> To manage your subscriptions to this and other Galaxy lists,
> please use the interface at:
>
>  http://lists.bx.psu.edu/
>



-- 
Istvan Albert
Associate Professor, Bioinformatics
Pennsylvania State University
http://www.personal.psu.edu/iua1/

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[galaxy-user] Question about file formats

2011-11-10 Thread Rena Zheng
Hi,
I uploaded a bed file to Galaxy and did some text manipulations.  I want to 
download the new file as a bed format that I can then open up in excel or a 
text editor.  However, when I save the data, it is a .tabular format that I 
cannot open with these programs.  What should I do?

Thanks,
Rena
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Re: [galaxy-user] Question about file formats

2011-11-10 Thread Peter Cock
On Thu, Nov 10, 2011 at 6:10 PM, Rena Zheng  wrote:
> Hi,
> I uploaded a bed file to Galaxy and did some text manipulations.  I want
> to download the new file as a bed format that I can then open up in excel
> or a text editor.  However, when I save the data, it is a .tabular format that
> I cannot open with these programs.  What should I do?
>
> Thanks,
> Rena

Try renaming the file to end with .tsv or .txt and Excel or your text editor
should be happy.

Peter

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[galaxy-user] my data files are missing from drop down menus

2011-11-10 Thread Maya Kasowski
Hello,

I uploaded a couple fastq files to Galaxy (Illumina GA2X sequencer).
The files uploaded successfully and are green in the history section.
However, neither file is visible in the drop down menus for any of the
tools. I'd appreciate your help with this.

Thank you,
Maya
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Re: [galaxy-user] my data files are missing from drop down menus

2011-11-10 Thread Dannon Baker
Maya,

Many tools require that fastq files specify that they are of sanger quality 
score.  Specifically, "Map with BWA for Illumina", etc.  If you click the 
pencil icon ("Edit Attributes") on your uploaded dataset and change the data 
type from 'fastq' to 'fastqsanger', you should be able to run your tools.

Thanks, and let me know if you have any other issues.

Dannon

On Nov 10, 2011, at 5:20 PM, Maya Kasowski wrote:

> Hello,
> 
> I uploaded a couple fastq files to Galaxy (Illumina GA2X sequencer).
> The files uploaded successfully and are green in the history section.
> However, neither file is visible in the drop down menus for any of the
> tools. I'd appreciate your help with this.
> 
> Thank you,
> Maya
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