[Histonet] Whole Mount Immuno Question (again)
Can anyone tell me where to buy a slide holder or chamber to perform manual whole mount immunos? Something like the slide master from Scytek would be great but I won't get my hopes up!! Thanks, ken Kenneth G Metzger HTL(ASCP) ARUP Labs Histology Supervisor 500 Chipeta Way Salt Lake City, Utah 84108-1221 kenneth.metz...@aruplab.com (801) 583-2787 x 3101 - -- The information transmitted by this e-mail and any included attachments are from ARUP Laboratories and are intended only for the recipient. The information contained in this message is confidential and may constitute inside or non-public information under international, federal, or state securities laws, or protected health information and is intended only for the use of the recipient. Unauthorized forwarding, printing, copying, distributing, or use of such information is strictly prohibited and may be unlawful. If you are not the intended recipient, please promptly delete this e-mail and notify the sender of the delivery error or you may call ARUP Laboratories Compliance Hot Line in Salt Lake City, Utah USA at (+1 (800) 522-2787 ext. 2100 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Benchmark XT
What kind of slides do you use?? Laurie Colbert -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Angela Bitting Sent: Thursday, April 09, 2009 6:14 PM To: Kari Bradshaw; histonet@lists.utsouthwestern.edu; Dana Spears; Joe Nocito; Phyllis Thaxton Subject: Re: [Histonet] Benchmark XT I just had the same problem with a bad lot of charged slides. It is, indeed, maddening. Joe Nocito jnoc...@satx.rr.com 4/8/2009 5:08 PM another issue could be the mixer blowing too hard. I've had that happen too. JTT - Original Message - From: Kari Bradshaw kbrads...@lcpath.com To: Dana Spears dspe...@mmci.org; histonet@lists.utsouthwestern.edu; Phyllis Thaxton dch...@yahoo.com Sent: Wednesday, April 08, 2009 2:13 PM Subject: RE: [Histonet] Benchmark XT We just recently went through a similar problem with irregular staining, light staining, sometimes no stain of any kind including counterstain,tissue falling off, and a handfull of false negative patient tissues, but positive controls (on the same slide). With two XT's running continuously it taken several days and everyone's help isolating the problemnot to mention several different lots numbers of slidesyikes! Next time I'll blame the slides right off the bat. Kari L. Bradshaw HT(ASCP) Laboratory Manager Lower Columbia Pathologists (360)425-5620 kbrads...@lcpath.com -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Dana Spears Sent: Wednesday, April 08, 2009 9:24 AM To: histonet@lists.utsouthwestern.edu; Phyllis Thaxton Subject: Re: [Histonet] Benchmark XT I would definitely check your slides - we have found that when we get a bad lot of charged slides, things start washing off on the XT - regardless of detection, antibodies, whatever. It was happening to us on some tissue types and not on others, but a new batch of charged slides did the trick. I've never had to dry 60 min in the oven - 20 does it for us when we aren't having slide issues. Also, if you are using any Sta-on or any adhesive in your waterbath with the charged slides it can cause the slides to sort of repel the tissue and negate the charged effect again certain tissues will fall off, others stay on Dana Spears, HTL(ASCP) Laboratory Manager Methodist Medical Center (309) 672-4930 (office) (309) 255-7214 (cell) (309) 279-3768 (fax) dspe...@mmci.org Phyllis Thaxton dch...@yahoo.com 4/8/2009 8:43:36 AM Is anyone out there having problems with tissue washing off the slides using the Ultraview kit from Ventana? Mainly we are having problems with needle biopsies washing off (prostate, liver). Fixation and processing is always the same 4-6 hours fixation, 3 hour biopsy processing run on VIP processor, cut no thicker than 4 microns, airdried at least 30 minutes, baked at 59-60 for at least one hour prior to staining. The instrument's calibrations have been checked and are fine. We never had this problem with the iView kit using the Nexes, doing pretreatments offline. Any help, ideas, info will be appreciated. Thanks, Phyllis Thaxton HT(ASCP)QIHC DCH Regional Medical Center Tuscaloosa, AL 35401 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet NOTICE: This message is a PRIVATE communication. This e-mail may contain confidential or proprietary information that may be considered legally privileged. It is intended only for the named recipient(s). If an addressing or transmission error has misdirected the e-mail, please notify the author by replying to this message. If you are not the named recipient, you are not authorized to use, disclose, distribute, copy, print, or rely on this e-mail, and should immediately delete it from your computer system. Thank you for your assistance with this matter. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this
[Histonet] A Friday afternoon Funny for the H-net folks :)
This was forwarded to me by a path lab friend--the link is clean and a nice giggle going into a holiday weekend. Cheryl Kerry, HT(ASCP) From: Dr. P.H. Sent: Friday, April 10, 2009 10:20 AM To: Subject: Critical Health Alert! As a physician, I am obligated to inform those individuals I care for about a critical health alert just released from the CDC in Atlanta , GA. The alert comes as the result of a huge study which was performed to try and explain a recent spike in unexplained illnesses. The researchers discovered that the inside of almost all computer screens are covered with bacteria, mites (and their feces), plus considerable microscopic industrial waste from the production of monitors overseas. All of this material seems to be the cause of the unexplained illnesses. Therefore, I urge you to immediately go to the link below to access a program specifically developed by the CDC to clean up and sterilize the inside of your monitor screen. Please, do this now so that I may rest at ease. Thank you. Phil Click on this link to clean the inside of your screen: http://www.raincitystory.com/flash/screenclean.swf ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] A Friday afternoon Funny for the H-net folks :)
Thanks, Cheryl! That's just what we needed after the week we have had here on the Histonet. Lynne Bell, HT(ASCP) Central Vermont Hospital 130 Fisher Road Barre, VT 05641 (802)371-4923 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Whole human feet
Denise, Thanks, someone else pointed out these images and they are indeed in the correct plane (http://www.nlm.nih.gov/research/visible/image/feet.jpg). I think these are 1 mm sections from a frozen body, unstained. We were actually hoping that we could process feet (procured from a company), section and stain the sections with the basic HE, modified Hart's, Picro sirius red and such like. Any help would be greatly appreciated. Thanks Yak-Nam On Fri, Apr 10, 2009 at 1:11 PM, Woodward, Denise denise.woodw...@uconn.edu wrote: I believe there is a computerized image file of an entire male and a female human body somewhere on the WWW. Maybe NIH?? These were whole mount histologic sections. Don't know if the plane is correct for your needs with regard to the sections of feet. News about it came out about 4-5 years ago. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernie Taupin Sent: Wednesday, April 08, 2009 12:07 AM To: Yak-Nam Wang; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Whole human feet No offense, dude, but GROSS. That's probably why nobody has bitten yet, in regards to this query. From: Yak-Nam Wang ynw...@u.washington.edu To: histonet@lists.utsouthwestern.edu Sent: Monday, April 6, 2009 8:04:54 PM Subject: [Histonet] Whole human feet Hello all, If possible, we would like to obtain histological sections of adult human feet (plane of the anterior-posterior surface). Does anyone know of labs/groups that have done this or something similar? Thank you for your help Yak-Nam Wang ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Whole human feet
As far as I know such a large thin section that also involves decalcifying hard bones has never been published. In the past E. Leitz (now Leica) manufactured sliding microtomes for large specimens but were used mostly to prepare thin brain sections embedded in celloidin but not for what you would like to do. If you are able to do it, for sure we all would like to know. René J. --- On Fri, 4/10/09, Yak-Nam Wang ynw...@u.washington.edu wrote: From: Yak-Nam Wang ynw...@u.washington.edu Subject: Re: [Histonet] Whole human feet To: Woodward, Denise denise.woodw...@uconn.edu Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: Friday, April 10, 2009, 4:26 PM Denise, Thanks, someone else pointed out these images and they are indeed in the correct plane (http://www.nlm.nih.gov/research/visible/image/feet.jpg). I think these are 1 mm sections from a frozen body, unstained. We were actually hoping that we could process feet (procured from a company), section and stain the sections with the basic HE, modified Hart's, Picro sirius red and such like. Any help would be greatly appreciated. Thanks Yak-Nam On Fri, Apr 10, 2009 at 1:11 PM, Woodward, Denise denise.woodw...@uconn.edu wrote: I believe there is a computerized image file of an entire male and a female human body somewhere on the WWW. Maybe NIH?? These were whole mount histologic sections. Don't know if the plane is correct for your needs with regard to the sections of feet. News about it came out about 4-5 years ago. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernie Taupin Sent: Wednesday, April 08, 2009 12:07 AM To: Yak-Nam Wang; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Whole human feet No offense, dude, but GROSS. That's probably why nobody has bitten yet, in regards to this query. From: Yak-Nam Wang ynw...@u.washington.edu To: histonet@lists.utsouthwestern.edu Sent: Monday, April 6, 2009 8:04:54 PM Subject: [Histonet] Whole human feet Hello all, If possible, we would like to obtain histological sections of adult human feet (plane of the anterior-posterior surface). Does anyone know of labs/groups that have done this or something similar? Thank you for your help Yak-Nam Wang ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] Whole human feet
Virtual autopsy web site? They have the whole body ct scanned and sliced. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Woodward, Denise Sent: Friday, April 10, 2009 3:11 PM To: 'Bernie Taupin'; Yak-Nam Wang; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Whole human feet I believe there is a computerized image file of an entire male and a female human body somewhere on the WWW. Maybe NIH?? These were whole mount histologic sections. Don't know if the plane is correct for your needs with regard to the sections of feet. News about it came out about 4-5 years ago. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernie Taupin Sent: Wednesday, April 08, 2009 12:07 AM To: Yak-Nam Wang; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Whole human feet No offense, dude, but GROSS. That's probably why nobody has bitten yet, in regards to this query. From: Yak-Nam Wang ynw...@u.washington.edu To: histonet@lists.utsouthwestern.edu Sent: Monday, April 6, 2009 8:04:54 PM Subject: [Histonet] Whole human feet Hello all, If possible, we would like to obtain histological sections of adult human feet (plane of the anterior-posterior surface). Does anyone know of labs/groups that have done this or something similar? Thank you for your help Yak-Nam Wang ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] Whole human feet
Thanks Rene, I have heard of the sliding microtomes and a colleague thought that as long as I could fix and decalcify, it might be possible with one of these. I fear I may have to pre-cut the foot in to manageable portions before processing. Yes, if I find out how to do it I will definitely let you know! Yak-Nam On Fri, Apr 10, 2009 at 1:34 PM, Rene J Buesa rjbu...@yahoo.com wrote: As far as I know such a large thin section that also involves decalcifying hard bones has never been published. In the past E. Leitz (now Leica) manufactured sliding microtomes for large specimens but were used mostly to prepare thin brain sections embedded in celloidin but not for what you would like to do. If you are able to do it, for sure we all would like to know. René J. --- On *Fri, 4/10/09, Yak-Nam Wang ynw...@u.washington.edu* wrote: From: Yak-Nam Wang ynw...@u.washington.edu Subject: Re: [Histonet] Whole human feet To: Woodward, Denise denise.woodw...@uconn.edu Cc: histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Date: Friday, April 10, 2009, 4:26 PM Denise, Thanks, someone else pointed out these images and they are indeed in the correct plane (http://www.nlm.nih.gov/research/visible/image/feet.jpg). I think these are 1 mm sections from a frozen body, unstained. We were actually hoping that we could process feet (procured from a company), section and stain the sections with the basic HE, modified Hart's, Picro sirius red and such like. Any help would be greatly appreciated. Thanks Yak-Nam On Fri, Apr 10, 2009 at 1:11 PM, Woodward, Denise denise.woodw...@uconn.edu wrote: I believe there is a computerized image file of an entire male and a female human body somewhere on the WWW. Maybe NIH?? These were whole mount histologic sections. Don't know if the plane is correct for your needs with regard to the sections of feet. News about it came out about 4-5 years ago. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto: histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bernie Taupin Sent: Wednesday, April 08, 2009 12:07 AM To: Yak-Nam Wang; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Whole human feet No offense, dude, but GROSS. That's probably why nobody has bitten yet, in regards to this query. From: Yak-Nam Wang ynw...@u.washington.edu To: histonet@lists.utsouthwestern.edu Sent: Monday, April 6, 2009 8:04:54 PM Subject: [Histonet] Whole human feet Hello all, If possible, we would like to obtain histological sections of adult human feet (plane of the anterior-posterior surface). Does anyone know of labs/groups that have done this or something similar? Thank you for your help Yak-Nam Wang ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing listhisto...@lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] HISTOS 5 processor
Hi Ann, How are things down there in Flodrida? Glad you get some money to buy something form Smilestone! Love, Bernie Taupin _ Hi, all We get some money recently and want to spend it on the HISTOS 5 proceesor from Smilestone. But we are not sure whether it is worth purchasing. Does anyone here have any experiences or suggestions of it? Thank you, Ann Fu University of Flodrida ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] List abuse
Bernie, list moderators, and people who are better at tracking things: Looking at the long headers (that contain all the IP addresses of senders/receivers) Bernie Taupin emails originate from UNIVERSITY OF MEDICINE AND DENTISTRY OF NEW JERSEY and an IP address in Brooklyn NYC. Also, one can note, looking at the histonet archives, that Bernie and aa (in the long header, one can see that aa also used the address lactose.intoler...@yahoo.com) use the same IPs and send messages within close proximity of each other. Both senders should clearly be ignored, and maybe someone from UMDNJ could look into the matter. Bernie is most likely in the medical profession, and posts to the list under his or her real name. This is clearly a case of professional misconduct and a misuse of public resources. I am not a IT professional, my analysis could be faulty, but, someone with the proper skills could track this down. -David On Apr 10, 2009, at 5:20 PM, Bernie Taupin wrote: Hi Ann, How are things down there in Flodrida? Glad you get some money to buy something form Smilestone! Love, Bernie Taupin _ Hi, all We get some money recently and want to spend it on the HISTOS 5 proceesor from Smilestone. But we are not sure whether it is worth purchasing. Does anyone here have any experiences or suggestions of it? Thank you, Ann Fu University of Flodrida ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
