[Histonet] Leica CM3600 Cryomacrotome XP

[Tina Van Meter]

Hello Histonetters,

Is anyone currently or in the past had experience operating the Leica
CM3600 Cryomacrotome XP?   I am looking for input on using this instrument.

Thanks,
Tina
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[Histonet] IHC Opening Dallas

[Pat Patterson]

IMMUNOHISTOCHEMISTRY TECHNICIAN

ProPath, a progressive, CAP accredited, high-volume pathology practice in 
Dallas, Texas is seeking an Immunohistochemistry Technician for its' 
Immunohistochemistry Lab.  Responsibilities include slide preparation (paraffin 
and frozen sections), IHC staining using our unique manual system, antibody 
titer preparation, equipment maintenance, supply/reagent inventory maintenance, 
and QC/QA recording.

The ideal candidate will have a minimum of 4 years Histology experience with 
paraffin microtomy with a variety of different tissue types, prefer at least 1 
- 2 years immunohistochemistry, immunofluorescence or in situ hybridization and 
frozen section experience.  Working knowledge of IHC theory required, hands on 
IHC performance desired.  If using an automated system we'll easily train you 
on our manual system.  HT (ASCP) or QIHC desired.

The hours for the position are 2:00 p.m. to 10:30 p.m. Monday through Friday.

ProPath utilizes leading technology and is a quality oriented pathology 
laboratory.  Benefits include medical, dental, Short and Long Term Disability 
insurance, a matched 401K plan and more!

Don't Follow the Leader!  Join the Leader!

To apply, please visit www.propath.com

EOE
M/F/Disabled/Veteran

Accessibility Accommodations
If you require an accommodation to navigate or apply to our careers site, 
please send your request to accessibil...@propath.com.



Pat Patterson, HTL(ASCP)
Supervisor, Immunohistochemistry
ProPath - The Leader in Pathology Services
1355 River Bend Drive
Dallas, TX 75247

214-237-1700 x 2027
214-237-1730 fax

To learn more about ProPath, please visit http://www.ProPath.com



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Re: [Histonet] GI Biopsies

[Cristi Rigazio]

Three levels one slide for all biopsies.  Colon polyps one section.

Sent from my iPhone

On Mar 13, 2014, at 11:29 AM, imhype...@aol.com wrote:

> 
> Good afternoon all,
> I was just curious about how your institutions handle GI biopsies, 
> specifically how many slides you cut off the bat.  We presently cut 2 levels 
> on each GI biopsy block, but I'm hearing that more and more places only cut 1 
> slide per GI biopsy block.  Please share what you are doing at your 
> establishment.
>Thank you 
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RE: [Histonet] RE: CAP Annual Results Comparison for FISH/ISH

[joelle weaver]

sorry , saw the Predictive, missed FISH - getting ready to do this myself. I 
would just call CAP, if I get to do that soon I will send along what 
information they supply.  




Joelle Weaver MAOM, HTL (ASCP) QIHC
 
> From: joellewea...@hotmail.com
> To: tbr...@holyredeemer.com; histonet@lists.utsouthwestern.edu
> Date: Thu, 13 Mar 2014 18:44:37 +
> Subject: RE: [Histonet] RE: CAP Annual Results Comparison for FISH/ISH
> CC: 
> 
> are you looking for the stats in the notes for ANP. 22970 (2012) ?
>  
> "overall ER negative breast ca ( invasive DCIS) should not exceed 30% ( lower 
> average 20-35% in post menopausal) , lower in well differentiated 
> tumors...etc."
>  
> I sure CAP can also send or repeat them to you if  you prefer to call them. 
>  
>  
> 
> 
> 
> 
> Joelle Weaver MAOM, HTL (ASCP) QIHC
>  
> > Date: Thu, 13 Mar 2014 14:06:23 -0400
> > From: tbr...@holyredeemer.com
> > To: histonet@lists.utsouthwestern.edu
> > Subject: [Histonet] RE: CAP Annual Results Comparison for FISH/ISH 
> > 
> > Try calling CAP.  They provided the benchmarks we use for our annual
> > statistics for ER/PR.  I don't have the recent CAP checklist handy, but
> > on the 9.25.2012 checklist, the benchmarks for ER/PR are published in
> > the notes section of the question. I hope this helps. Sincerely, Terri
> > 
> > Terri L. Braud, HT(ASCP)
> > Anatomic Pathology Supervisor
> > Holy Redeemer Hospital Laboratory
> > 1648 Huntingdon Pike
> > Meadowbrook, PA 19046
> > Ph: 215-938-3676
> > Fax: 215-938-3874
> > 
> > Message: 3
> > Date: Wed, 12 Mar 2014 18:40:53 +
> > From: "O'neil, Beth" 
> > Subject: [Histonet] CAP Annual Results Comparison for FISH/ISH
> > Would fellow Histonetters be able to explain how they answer the
> > following CAP question:
> > ANP.22970 For immunohistochemical and FISH/ISH tests that provide
> > independent predictive information, the laboratory at least annually
> > compares its patient results with published benchmarks, and evaluates
> > interobserver variability among the pathologists in the laboratory.
> > Where would one even find published benchmarks?  Thank you
> > 
> > Beth Ann O'Neil, MT(ASCP)SC, HTL, QIHC
> > Histology Supervisor/Technical Specialist
> > West Virginia University Hospitals
> > one...@wvuhealthcare.com
> > 304-293-7629 (office)
> > 304-293-6014 (lab)
> > 
> > **
> > -
> > 
> > 
> > 
> > CONFIDENTIALITY NOTICE:
> > 
> > This E-Mail is intended only for the use of the individual or entity to 
> > which
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> > distribute any part of it or retain any copies, and delete the original 
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> > Please notify the sender of any error by E-Mail.
> > 
> > Thank you for your cooperation.
> > 
> > 
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RE: [Histonet] RE: CAP Annual Results Comparison for FISH/ISH

[joelle weaver]

are you looking for the stats in the notes for ANP. 22970 (2012) ?
 
"overall ER negative breast ca ( invasive DCIS) should not exceed 30% ( lower 
average 20-35% in post menopausal) , lower in well differentiated tumors...etc."
 
I sure CAP can also send or repeat them to you if  you prefer to call them. 
 
 




Joelle Weaver MAOM, HTL (ASCP) QIHC
 
> Date: Thu, 13 Mar 2014 14:06:23 -0400
> From: tbr...@holyredeemer.com
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] RE: CAP Annual Results Comparison for FISH/ISH 
> 
> Try calling CAP.  They provided the benchmarks we use for our annual
> statistics for ER/PR.  I don't have the recent CAP checklist handy, but
> on the 9.25.2012 checklist, the benchmarks for ER/PR are published in
> the notes section of the question. I hope this helps. Sincerely, Terri
> 
> Terri L. Braud, HT(ASCP)
> Anatomic Pathology Supervisor
> Holy Redeemer Hospital Laboratory
> 1648 Huntingdon Pike
> Meadowbrook, PA 19046
> Ph: 215-938-3676
> Fax: 215-938-3874
> 
> Message: 3
> Date: Wed, 12 Mar 2014 18:40:53 +
> From: "O'neil, Beth" 
> Subject: [Histonet] CAP Annual Results Comparison for FISH/ISH
> Would fellow Histonetters be able to explain how they answer the
> following CAP question:
> ANP.22970 For immunohistochemical and FISH/ISH tests that provide
> independent predictive information, the laboratory at least annually
> compares its patient results with published benchmarks, and evaluates
> interobserver variability among the pathologists in the laboratory.
> Where would one even find published benchmarks?  Thank you
> 
> Beth Ann O'Neil, MT(ASCP)SC, HTL, QIHC
> Histology Supervisor/Technical Specialist
> West Virginia University Hospitals
> one...@wvuhealthcare.com
> 304-293-7629 (office)
> 304-293-6014 (lab)
> 
> **
> -
> 
> 
> 
> CONFIDENTIALITY NOTICE:
> 
> This E-Mail is intended only for the use of the individual or entity to which
> it was sent. It may contain information that is privileged and/or 
> confidential,
> and the use or disclosure of such information may also be restricted under 
> applicable
> federal and state law. If you received this communication in error, please do 
> not
> distribute any part of it or retain any copies, and delete the original 
> E-Mail.
> Please notify the sender of any error by E-Mail.
> 
> Thank you for your cooperation.
> 
> 
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RE: [Histonet] GI Biopsies

[Blazek, Linda]

3 levels - 1 slide

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
imhype...@aol.com
Sent: Thursday, March 13, 2014 2:30 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] GI Biopsies


Good afternoon all,
 I was just curious about how your institutions handle GI biopsies, 
specifically how many slides you cut off the bat.  We presently cut 2 levels on 
each GI biopsy block, but I'm hearing that more and more places only cut 1 
slide per GI biopsy block.  Please share what you are doing at your 
establishment.
Thank you 
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RE: [Histonet] RE: MUM-1

[joelle weaver]

I have the bond and I use the BIO SB concentrate, RaBMab, EP190 at 1:50 and it 
stains great on tonsil and kidney tubules.  




Joelle Weaver MAOM, HTL (ASCP) QIHC
 
> From: lseb...@uwhealth.org
> To: cda...@che-east.org; histonet@lists.utsouthwestern.edu
> Date: Thu, 13 Mar 2014 16:19:11 +
> CC: 
> Subject: [Histonet] RE: MUM-1
> 
> This is our protocol for the Ultra; maybe it will help.
> 
> 64" CC1, 32" incubation (MUM-1, 760-4529) @ 36 degrees, Hem II/4".  This is 
> with UltraView DAB detection.  We use tonsil as well however we validated 
> with HD, LN, GI, tonsil, etc.
> 
> Good luck!
> 
> Linda A. Sebree 
> University of Wisconsin Hospital & Clinics 
> IHC/ISH Laboratory 
> 600 Highland Ave. 
> Madison, WI 53792 
> (608)265-6596 
> FAX: (608)262-7174 
> 
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu 
> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Davis, Cassie
> Sent: Thursday, March 13, 2014 8:54 AM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] MUM-1
> 
> Good morning Histonet Folks,
> 
>  I am hoping one of you will help me. I am in the process of 
> optimizing an IHC protocol on the MUM-1 antibody on paraffin tissue for the 
> Benchmark XTstainer and I am not thrilled with the results I am getting. I 
> have tried the "usual adjustments" and the results are less than optimal in 
> my opinion. I am using a normal tonsil control right now but if you have 
> another suggestion please do not hesitate to recommend. I am praying somebody 
> might have done this before and would be willing to share their staining 
> protocol or tips with this.
> 
> Cassandra Davis
> cda...@che-east.org
> 302-575-8095
> 
> 
> 
> Confidentiality Notice:
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> unauthorized review, use, disclosure, or distribution is prohibited. If you 
> are not the intended recipient, please delete this message, and reply to the 
> sender regarding the error in a separate email.
>  
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[Histonet] RE: Cornflakes

[Terri Braud]

As a long time user of film (though sadly, no longer) we used to see
what sounds like this artifact when the Xylene dispenser was not
dispensing enough xylene and the slides were not wet enough.  It drove
us crazy until we realized that through the years, the xylene dispensing
knob had become very loose and would readjust itself at the slightest
touch or vibration. We liked our Xylene to be dispensing rapidly, just
short of a steady stream, so constantly checked it.
Also, if your slides are not completely dehydrated before clearing in
Xylene, you are just asking for problems, if not now, then years down
the road when the tape will pull off the slide, taking your tissue with
it.
Been there, done that.  Terri

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Holy Redeemer Hospital Laboratory
1648 Huntingdon Pike
Meadowbrook, PA 19046
Ph: 215-938-3676
Fax: 215-938-3874

Message: 10
Date: Thu, 13 Mar 2014 02:59:38 +
From: Sharon Scalise 
Subject: [Histonet] RE: Cornflaking artifact
To: "histonet@lists.utsouthwestern.edu"

I am looking for help with "cornflaking" (tiny, brown dry spots under
coverslip)artifact.  We have been using fresh xylene on our stainer and
coverslipper, cleaned and wiped all containers dry before filling, tried
different lots of coverslipping film and had service on our coverslipper
to make sure it was functioning properly, including the xylene drip.  We
continue to have this artifact and it is driving us crazy.  It is
sporadic with no pattern of tissue type or placement on the slide.
Sometimes it lands on tissue other times not.  Most of the time when we
remove the coverslip and re-coverslip it goes away (I am assuming
because the acetone removes any minute amounts of water that may be
present).  We just cannot figure out where the water is coming from.
Has anyone seen this artifact while using the drying step on the prisma
stainer?  We just recently started using the drying on some slides and I
am thinking maybe it is causing humidity???  I cannot say for a fact
that

-



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Re: [Histonet] GI Biopsies

[Sue]

We actually cut 2 slides with 4 levels 2 on each slide.  Out pathologists were 
unwilling to only 

cut one slide 




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RE: [Histonet] GI Biopsies

[Weems, Joyce K.]

We do 3 levels on one slide.

Joyce Weems
Pathology Manager
678-843-7376 Phone
678-843-7831 Fax
joyce.we...@emoryhealthcare.org



www.saintjosephsatlanta.org
5665 Peachtree Dunwoody Road
Atlanta, GA 30342

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regarding the error in a separate email.


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of 
imhype...@aol.com
Sent: Thursday, March 13, 2014 2:30 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] GI Biopsies


Good afternoon all,
 I was just curious about how your institutions handle GI biopsies, 
specifically how many slides you cut off the bat.  We presently cut 2 levels on 
each GI biopsy block, but I'm hearing that more and more places only cut 1 
slide per GI biopsy block.  Please share what you are doing at your 
establishment.
Thank you
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Re: [Histonet] Re: Cornflaking

[srishan]

We are all putting out what worked for us.  You should  try some of these 
suggestions and see what works for you.  The suggestion for improving poor 
dehydration was suggested by technical support  from Sakura.  Cornflaking 
was irritating so many of our pathologist, we had to get technical support 
from Sakura to see how this could be resolved.  In OUR hospital changing 
the tape worked!!!  Good Luck.!!!

Mala

Nirmala Srishan
Holy Name Medical Center.



From:   joe joe 
To: Beth Cox 
Cc: "histonet@lists.utsouthwestern.edu" 

Date:   03/13/2014 02:20 PM
Subject:Re: [Histonet] Re: Cornflaking
Sent by:histonet-boun...@lists.utsouthwestern.edu



Why doesn't someone call Kellogg's ?




> On Mar 13, 2014, at 12:10 PM, "Beth Cox"  wrote:
> 
> Sharon & other Histonetters,
> 
> Cornflaking is literally microscopic air trapped under the coverslip. It
> doesn't have anything to do with poor dehydration and trapped water. 
Thus
> it can be caused by partial drying out before the coverslipping.
> 
> The things you need to look at to eradicate the problem is:
> 1.  Keep the slides wet before coverslipping (obviously)
> 2.  Consider increasing the amount of xylene deposited on the slide for
> tape coverslippers.
> 3.  For glass coverslippers, consider increasing the xylene and 
increasing
> or changing the mounting media.
> 
> Cornflaking tends to happen more on slides/sections with rough 
"topography"
> on the section (the more rough it is, the more nooks & crannies to trap 
the
> air).  So anything that would give the section a more rough surface 
would
> increase the tendency to cornflake; such as: section lifting. section
> thickness, even chatter in the sections.  Think about things that would
> affect the section - for example, are you using a different brand of 
blade
> on the microtome?
> 
> Hope this helps,
> 
> Beth Cox, HTL/SCT(ASCP)QIHC
> 
> 
> 
> 
> Message: 15
> Date: Thu, 13 Mar 2014 11:59:06 -0400
> From: sris...@mail.holyname.org
> Subject: Re: [Histonet] RE: Cornflaking artifact
> To: "HERRINGTON, SHEILA" 
> Cc: "histonet@lists.utsouthwestern.edu"
>,Sharon Scalise
>,
> histonet-boun...@lists.utsouthwestern.edu,
>'Laurie Colbert' 
> Message-ID:
><
> of46844f54.2f7fc911-on85257c9a.00575cb9-85257c9a.0057c...@holyname.org>
> Content-Type: text/plain; charset="US-ASCII"
> We had this problem several years ago.  We  were using the sakura tapes
> with the coverslipper.
> We did the following:
> Last three alcohols were changes frequently.
> Slides should be not dry when loading on coverslipper.
> If you could load two racks at a time, only load one.  By this way the
> slides in the second rack will not dry out.
> Finally, change the tapes from sakura to Mercedes Medical tapes.
> Mala
> Nirmala Srishan
> Holy Name Medical Center
> 
> 
> From:   "HERRINGTON, SHEILA" 
> To: 'Laurie Colbert' , Sharon Scalise
> , "histonet@lists.utsouthwestern.edu"
> 
> Date:   03/13/2014 11:43 AM
> Subject:[Histonet] RE: Cornflaking artifact
> Sent by:histonet-boun...@lists.utsouthwestern.edu
> 
> We also have recently started to see this artifact more than ever 
before,
> and nothing in our process has changed.  We have tried everything to
> correct to no avail.  Wonder if it is possible to be a change in some 
type
> of supply, either xylene or coverslipping film.  Something has changed 
but
> am at a loss as to what.
> 
> Sheila Herrington
> Technical Lead Histopathology and Immunohistochemistry
> Kelowna General Hospital
> 2268 Pandosy Street, Kelowna, B.C. V1Y 1T2
> 250-862-4300 ext 7587 or 7510
> sheila.herring...@interiorhealth.ca
> 
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu [
> mailto:histonet-boun...@lists.utsouthwestern.edu
]
> On Behalf Of Laurie
> Colbert
> Sent: Thursday, March 13, 2014 6:30 AM
> To: Sharon Scalise; histonet@lists.utsouthwestern.edu
> Subject: [Histonet] RE: Cornflaking artifact
> You will also see the cornflaking if your tissue is lifting off of the
> slide at all.  We used to get this more often on hard, decal specimens
> than on other specimens.  We used the film to coverslip.  If you remove
> the film from the problem slides and recoverslip conventionally with 
extra
> mountant and glass coverslips, I'm sure you will not see the artifact.
> Laurie Colbert
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu [
> mailto:histonet-boun...@lists.utsouthwestern.edu
]
> On Behalf Of Sharon
> Scalise
> Sent: Wednesday, March 12, 2014 8:00 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] RE: Cornflaking artifact
> I am looking for help with "cornflaking" (tiny, brown dry spots under
> coverslip)artifact.  We have been using fresh xylene on our stainer and
> coverslipper, cleaned and wiped all containers dry before filling, tried
> different lots of coverslipping film and had service on our coverslipper
> to make sure it was functioning properly

[Histonet] GI Biopsies

[imhyper13]

Good afternoon all,
 I was just curious about how your institutions handle GI biopsies, 
specifically how many slides you cut off the bat.  We presently cut 2 levels on 
each GI biopsy block, but I'm hearing that more and more places only cut 1 
slide per GI biopsy block.  Please share what you are doing at your 
establishment.
Thank you 
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[Histonet] Re: Cornflaking

[Beth Cox]

Sharon & other Histonetters,

Cornflaking is literally microscopic air trapped under the coverslip. It
doesn't have anything to do with poor dehydration and trapped water. Thus
it can be caused by partial drying out before the coverslipping.

The things you need to look at to eradicate the problem is:
1.  Keep the slides wet before coverslipping (obviously)
2.  Consider increasing the amount of xylene deposited on the slide for
tape coverslippers.
3.  For glass coverslippers, consider increasing the xylene and increasing
or changing the mounting media.

Cornflaking tends to happen more on slides/sections with rough "topography"
on the section (the more rough it is, the more nooks & crannies to trap the
air).  So anything that would give the section a more rough surface would
increase the tendency to cornflake; such as: section lifting. section
thickness, even chatter in the sections.  Think about things that would
affect the section - for example, are you using a different brand of blade
on the microtome?

Hope this helps,

Beth Cox, HTL/SCT(ASCP)QIHC




Message: 15
Date: Thu, 13 Mar 2014 11:59:06 -0400
From: sris...@mail.holyname.org
Subject: Re: [Histonet] RE: Cornflaking artifact
To: "HERRINGTON, SHEILA" 
Cc: "histonet@lists.utsouthwestern.edu"
,Sharon Scalise
,
histonet-boun...@lists.utsouthwestern.edu,
'Laurie Colbert' 
Message-ID:
<
of46844f54.2f7fc911-on85257c9a.00575cb9-85257c9a.0057c...@holyname.org>
Content-Type: text/plain; charset="US-ASCII"
We had this problem several years ago.  We  were using the sakura tapes
with the coverslipper.
We did the following:
Last three alcohols were changes frequently.
Slides should be not dry when loading on coverslipper.
If you could load two racks at a time, only load one.  By this way the
slides in the second rack will not dry out.
Finally, change the tapes from sakura to Mercedes Medical tapes.
Mala
Nirmala Srishan
Holy Name Medical Center


From:   "HERRINGTON, SHEILA" 
To: 'Laurie Colbert' , Sharon Scalise
, "histonet@lists.utsouthwestern.edu"

Date:   03/13/2014 11:43 AM
Subject:[Histonet] RE: Cornflaking artifact
Sent by:histonet-boun...@lists.utsouthwestern.edu

We also have recently started to see this artifact more than ever before,
and nothing in our process has changed.  We have tried everything to
correct to no avail.  Wonder if it is possible to be a change in some type
of supply, either xylene or coverslipping film.  Something has changed but
am at a loss as to what.

Sheila Herrington
Technical Lead Histopathology and Immunohistochemistry
Kelowna General Hospital
2268 Pandosy Street, Kelowna, B.C. V1Y 1T2
250-862-4300 ext 7587 or 7510
sheila.herring...@interiorhealth.ca

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu [
mailto:histonet-boun...@lists.utsouthwestern.edu]
On Behalf Of Laurie
Colbert
Sent: Thursday, March 13, 2014 6:30 AM
To: Sharon Scalise; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Cornflaking artifact
You will also see the cornflaking if your tissue is lifting off of the
slide at all.  We used to get this more often on hard, decal specimens
than on other specimens.  We used the film to coverslip.  If you remove
the film from the problem slides and recoverslip conventionally with extra
mountant and glass coverslips, I'm sure you will not see the artifact.
Laurie Colbert
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu [
mailto:histonet-boun...@lists.utsouthwestern.edu]
On Behalf Of Sharon
Scalise
Sent: Wednesday, March 12, 2014 8:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Cornflaking artifact
I am looking for help with "cornflaking" (tiny, brown dry spots under
coverslip)artifact.  We have been using fresh xylene on our stainer and
coverslipper, cleaned and wiped all containers dry before filling, tried
different lots of coverslipping film and had service on our coverslipper
to make sure it was functioning properly, including the xylene drip.  We
continue to have this artifact and it is driving us crazy.  It is sporadic
with no pattern of tissue type or placement on the slide.  Sometimes it
lands on tissue other times not.  Most of the time when we remove the
coverslip and re-coverslip it goes away (I am assuming because the acetone
removes any minute amounts of water that may be present).  We just cannot
figure out where the water is coming from.  Has anyone seen this artifact
while using the drying step on the prisma stainer?  We just recently
started using the drying on some slides and I am thinking maybe it is
causing humidity???  I cannot say for a fact that our "cornflaking"
started at the same time, but it is suspicious. HELP!
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Re: [Histonet] Re: Cornflaking

[joe joe]

Why doesn't someone call Kellogg's ?




> On Mar 13, 2014, at 12:10 PM, "Beth Cox"  wrote:
> 
> Sharon & other Histonetters,
> 
> Cornflaking is literally microscopic air trapped under the coverslip. It
> doesn't have anything to do with poor dehydration and trapped water. Thus
> it can be caused by partial drying out before the coverslipping.
> 
> The things you need to look at to eradicate the problem is:
> 1.  Keep the slides wet before coverslipping (obviously)
> 2.  Consider increasing the amount of xylene deposited on the slide for
> tape coverslippers.
> 3.  For glass coverslippers, consider increasing the xylene and increasing
> or changing the mounting media.
> 
> Cornflaking tends to happen more on slides/sections with rough "topography"
> on the section (the more rough it is, the more nooks & crannies to trap the
> air).  So anything that would give the section a more rough surface would
> increase the tendency to cornflake; such as: section lifting. section
> thickness, even chatter in the sections.  Think about things that would
> affect the section - for example, are you using a different brand of blade
> on the microtome?
> 
> Hope this helps,
> 
> Beth Cox, HTL/SCT(ASCP)QIHC
> 
> 
> 
> 
> Message: 15
> Date: Thu, 13 Mar 2014 11:59:06 -0400
> From: sris...@mail.holyname.org
> Subject: Re: [Histonet] RE: Cornflaking artifact
> To: "HERRINGTON, SHEILA" 
> Cc: "histonet@lists.utsouthwestern.edu"
>,Sharon Scalise
>,
> histonet-boun...@lists.utsouthwestern.edu,
>'Laurie Colbert' 
> Message-ID:
><
> of46844f54.2f7fc911-on85257c9a.00575cb9-85257c9a.0057c...@holyname.org>
> Content-Type: text/plain; charset="US-ASCII"
> We had this problem several years ago.  We  were using the sakura tapes
> with the coverslipper.
> We did the following:
> Last three alcohols were changes frequently.
> Slides should be not dry when loading on coverslipper.
> If you could load two racks at a time, only load one.  By this way the
> slides in the second rack will not dry out.
> Finally, change the tapes from sakura to Mercedes Medical tapes.
> Mala
> Nirmala Srishan
> Holy Name Medical Center
> 
> 
> From:   "HERRINGTON, SHEILA" 
> To: 'Laurie Colbert' , Sharon Scalise
> , "histonet@lists.utsouthwestern.edu"
> 
> Date:   03/13/2014 11:43 AM
> Subject:[Histonet] RE: Cornflaking artifact
> Sent by:histonet-boun...@lists.utsouthwestern.edu
> 
> We also have recently started to see this artifact more than ever before,
> and nothing in our process has changed.  We have tried everything to
> correct to no avail.  Wonder if it is possible to be a change in some type
> of supply, either xylene or coverslipping film.  Something has changed but
> am at a loss as to what.
> 
> Sheila Herrington
> Technical Lead Histopathology and Immunohistochemistry
> Kelowna General Hospital
> 2268 Pandosy Street, Kelowna, B.C. V1Y 1T2
> 250-862-4300 ext 7587 or 7510
> sheila.herring...@interiorhealth.ca
> 
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu [
> mailto:histonet-boun...@lists.utsouthwestern.edu]
> On Behalf Of Laurie
> Colbert
> Sent: Thursday, March 13, 2014 6:30 AM
> To: Sharon Scalise; histonet@lists.utsouthwestern.edu
> Subject: [Histonet] RE: Cornflaking artifact
> You will also see the cornflaking if your tissue is lifting off of the
> slide at all.  We used to get this more often on hard, decal specimens
> than on other specimens.  We used the film to coverslip.  If you remove
> the film from the problem slides and recoverslip conventionally with extra
> mountant and glass coverslips, I'm sure you will not see the artifact.
> Laurie Colbert
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu [
> mailto:histonet-boun...@lists.utsouthwestern.edu]
> On Behalf Of Sharon
> Scalise
> Sent: Wednesday, March 12, 2014 8:00 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] RE: Cornflaking artifact
> I am looking for help with "cornflaking" (tiny, brown dry spots under
> coverslip)artifact.  We have been using fresh xylene on our stainer and
> coverslipper, cleaned and wiped all containers dry before filling, tried
> different lots of coverslipping film and had service on our coverslipper
> to make sure it was functioning properly, including the xylene drip.  We
> continue to have this artifact and it is driving us crazy.  It is sporadic
> with no pattern of tissue type or placement on the slide.  Sometimes it
> lands on tissue other times not.  Most of the time when we remove the
> coverslip and re-coverslip it goes away (I am assuming because the acetone
> removes any minute amounts of water that may be present).  We just cannot
> figure out where the water is coming from.  Has anyone seen this artifact
> while using the drying step on the prisma stainer?  We just recently
> started using the drying on some slides and I am thinking maybe it is
> causing humidity???  I cannot say for a fact that our "cornfl

[Histonet] RE: CAP Annual Results Comparison for FISH/ISH

[Terri Braud]

Try calling CAP.  They provided the benchmarks we use for our annual
statistics for ER/PR.  I don't have the recent CAP checklist handy, but
on the 9.25.2012 checklist, the benchmarks for ER/PR are published in
the notes section of the question. I hope this helps. Sincerely, Terri

Terri L. Braud, HT(ASCP)
Anatomic Pathology Supervisor
Holy Redeemer Hospital Laboratory
1648 Huntingdon Pike
Meadowbrook, PA 19046
Ph: 215-938-3676
Fax: 215-938-3874

Message: 3
Date: Wed, 12 Mar 2014 18:40:53 +
From: "O'neil, Beth" 
Subject: [Histonet] CAP Annual Results Comparison for FISH/ISH
Would fellow Histonetters be able to explain how they answer the
following CAP question:
ANP.22970 For immunohistochemical and FISH/ISH tests that provide
independent predictive information, the laboratory at least annually
compares its patient results with published benchmarks, and evaluates
interobserver variability among the pathologists in the laboratory.
Where would one even find published benchmarks?  Thank you

Beth Ann O'Neil, MT(ASCP)SC, HTL, QIHC
Histology Supervisor/Technical Specialist
West Virginia University Hospitals
one...@wvuhealthcare.com
304-293-7629 (office)
304-293-6014 (lab)

**
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Re: [Histonet] RE: Cornflaking artifact

[Sue]

We had this issue when we were using the tape from Mercedes Medical and went 
back to 

Sakura tape and it went away.  It could also be associated with humidity.  



STP 

TJUH 




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[Histonet] request for surplus equipment

[sarah holmes]

Dear Histonet,

A colleague in Manicaland Province, Mutare, Zimbabwe is in need of a 
thermocycler if anyone has an old one that they would be able to donate.


The colleague is the Coordinator for Laboratory Training at Africa 
University.She and the university work to provide opportunities for 
students from all over Africa to learn clinical laboratory science skills.


Please contact me off list if you can help and I will arrange shipping 
and put you in contact with the university!


Thank you,

--
Sarah Holmes
Laboratory Manager
Laboratory for Kidney Pathology, Inc.
1916 Patterson St, Suite 501
Nashville, TN 37203
615 321 5729

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[Histonet] IHC without wash buffer

[Goins, Tresa]

In a continuing effort to limit the volume of reagent used in each step of a 
manual IHC, I have tried TBS and TBST on slides with barriers (expensive) and 
without barriers.
The results were not stellar with the barrier slides - the reagent still 
escapes.  We dewax with hot detergent (may be a contributing factor) and the 
Tween 20 in the TBST definitely alters the hydrophobicity of the barrier, an 
effect that is not reversed with a water wash.

Consequently, I have resorted to omitting the buffer wash steps and using 
distilled water only.  The slide surface remains "water repellent" and the 
added IHC reagents form a pool over the tissue sections.
The detection method is a polymer-HRP and there is no increase in background 
staining in the tissue or on the slide surface.

I am assuming that the IHC reagents are prepared in the "optimum" suspension 
liquid and a buffer is not required.  So, I am interested in hearing from 
anyone why this is a bad idea.

Thanks,

Tresa






[X]
[X]




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[Histonet] New Jersey Meeting

[French, Michele]

I just wanted to send a reminder that this Friday, March 15th is the 
registration deadline for the NJSH Spring Meeting planned for April 5th in 
Mount Laurel, NJ. There will be four 90 minute seminars entitled "Respiratory 
Histopathology", "Pathology of the Urinary Tract", "Animal Model Development to 
Aid in the Understanding of Human Atherosclerosis", and "Predictive Testing in 
Non-small Cell Lung Cancer: Testing Protocols, Specimen Requirements and Near 
Future Techniques, Notably Next Generation Sequencing".  The cost is ONLY $90 
for the day and you will receive 6 CEUs for attending! For more information and 
to register on-line, please visit our website http://njsh.org/njsh/



This message (including any attachments) may contain confidential, proprietary, 
privileged and/or private information. The information is intended to be for 
the use of the individual or entity designated above. If you are not the 
intended recipient of this message, please notify the sender immediately, and 
delete the message and any attachments. Any disclosure, reproduction, 
distribution or other use of this message or any attachments by an individual 
or entity other than the intended recipient is prohibited.
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[Histonet] RE: Cornflaking artifact

[Lavigne, Lisa]

We have changed lots of tape and still are having this artifact.
Lisa LaVigne CT, MB (ASCP)
Pathology Manager
St. Peter's Health Partners
315 S. Manning Blvd.
Albany, NY 12208
Phone: 518-525-5274
Fax: 518-525-6750
Email: lisa.lavi...@sphp.com

From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Metzger, Kenneth 
[kenneth.metz...@aruplab.com]
Sent: Thursday, March 13, 2014 12:13 PM
To: HERRINGTON, SHEILA; 'Laurie Colbert'; Sharon Scalise; 
histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Cornflaking artifact

Can anyone share the lot # on their coverslipping film (Sakura)? We are seeing 
this as well out of the blue..haven't changed a thing.

Kenneth G Metzger HTL(ASCP)
Histology Supervisor
ARUP Labs
Salt Lake City, Utah
Phone: (801)583-2787 ext. 3101
Fax: (801) 584-5244
Email: kenneth.metz...@aruplab.com


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of HERRINGTON, 
SHEILA
Sent: Thursday, March 13, 2014 9:42 AM
To: 'Laurie Colbert'; Sharon Scalise; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Cornflaking artifact

We also have recently started to see this artifact more than ever before, and 
nothing in our process has changed.  We have tried everything to correct to no 
avail.  Wonder if it is possible to be a change in some type of supply, either 
xylene or coverslipping film.  Something has changed but am at a loss as to 
what.


Sheila Herrington
Technical Lead Histopathology and Immunohistochemistry Kelowna General Hospital
2268 Pandosy Street, Kelowna, B.C. V1Y 1T2
250-862-4300 ext 7587 or 7510
sheila.herring...@interiorhealth.ca



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert
Sent: Thursday, March 13, 2014 6:30 AM
To: Sharon Scalise; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Cornflaking artifact

You will also see the cornflaking if your tissue is lifting off of the slide at 
all.  We used to get this more often on hard, decal specimens than on other 
specimens.  We used the film to coverslip.  If you remove the film from the 
problem slides and recoverslip conventionally with extra mountant and glass 
coverslips, I'm sure you will not see the artifact.

Laurie Colbert

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sharon Scalise
Sent: Wednesday, March 12, 2014 8:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Cornflaking artifact

I am looking for help with "cornflaking" (tiny, brown dry spots under 
coverslip)artifact.  We have been using fresh xylene on our stainer and 
coverslipper, cleaned and wiped all containers dry before filling, tried 
different lots of coverslipping film and had service on our coverslipper to 
make sure it was functioning properly, including the xylene drip.  We continue 
to have this artifact and it is driving us crazy.  It is sporadic with no 
pattern of tissue type or placement on the slide.  Sometimes it lands on tissue 
other times not.  Most of the time when we remove the coverslip and 
re-coverslip it goes away (I am assuming because the acetone removes any minute 
amounts of water that may be present).  We just cannot figure out where the 
water is coming from.  Has anyone seen this artifact while using the drying 
step on the prisma stainer?  We just recently started using the drying on some 
slides and I am thinking maybe it is causing humidity???  I cannot say for a 
fact that our "cornflaking" started at the same time, but it is suspicious. 
HELP!

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Wait, Trevor 
Jordan
Sent: Wednesday, March 12, 2014 3:57 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Paraffin type and Tetracycline labelling Questions

For those who have done Decalcified bone processing with paraffinwhat is 
the best type of paraffin that you guys are familiar with?

Also, if you are wanting to see a tetracycline label on the bone for bone 
turnover, must undecalcified sections be used? How for a double tetracycline 
label?


Trevor Jordan Wait
University of Texas Health Science Center, San Antonio Class of 2017 MD 
Candidate Abilene Christian University Class of 2013 Graduate B.S.  
Biochemistry ___
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His

RE: [Histonet] RE: Cornflaking artifact

[WILLIAM DESALVO]

Long time and high volume user of the Sakura Tape. Check the Xylene drops. We 
coverslip thousands of slides daily and have seen no isssue with the tape lots. 
Our experience shows some cornflaking when the slide does not receive enough 
xylens to wet the slide properly.

William DeSalvo, BS HTL(ASCP)
 
> From: kenneth.metz...@aruplab.com
> To: sheila.herring...@interiorhealth.ca; lcolb...@pathmdlabs.com; 
> sscal...@beaumont.edu; histonet@lists.utsouthwestern.edu
> Date: Thu, 13 Mar 2014 16:13:24 +
> CC: 
> Subject: [Histonet] RE: Cornflaking artifact
> 
> Can anyone share the lot # on their coverslipping film (Sakura)? We are 
> seeing this as well out of the blue..haven't changed a thing.
> 
> Kenneth G Metzger HTL(ASCP)
> Histology Supervisor
> ARUP Labs
> Salt Lake City, Utah
> Phone: (801)583-2787 ext. 3101
> Fax: (801) 584-5244
> Email: kenneth.metz...@aruplab.com
> 
> 
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu 
> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of HERRINGTON, 
> SHEILA
> Sent: Thursday, March 13, 2014 9:42 AM
> To: 'Laurie Colbert'; Sharon Scalise; histonet@lists.utsouthwestern.edu
> Subject: [Histonet] RE: Cornflaking artifact
> 
> We also have recently started to see this artifact more than ever before, and 
> nothing in our process has changed.  We have tried everything to correct to 
> no avail.  Wonder if it is possible to be a change in some type of supply, 
> either xylene or coverslipping film.  Something has changed but am at a loss 
> as to what.
> 
> 
> Sheila Herrington
> Technical Lead Histopathology and Immunohistochemistry Kelowna General 
> Hospital
> 2268 Pandosy Street, Kelowna, B.C. V1Y 1T2
> 250-862-4300 ext 7587 or 7510
> sheila.herring...@interiorhealth.ca
> 
> 
> 
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu 
> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert
> Sent: Thursday, March 13, 2014 6:30 AM
> To: Sharon Scalise; histonet@lists.utsouthwestern.edu
> Subject: [Histonet] RE: Cornflaking artifact
> 
> You will also see the cornflaking if your tissue is lifting off of the slide 
> at all.  We used to get this more often on hard, decal specimens than on 
> other specimens.  We used the film to coverslip.  If you remove the film from 
> the problem slides and recoverslip conventionally with extra mountant and 
> glass coverslips, I'm sure you will not see the artifact.
> 
> Laurie Colbert
> 
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu 
> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sharon Scalise
> Sent: Wednesday, March 12, 2014 8:00 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] RE: Cornflaking artifact
> 
> I am looking for help with "cornflaking" (tiny, brown dry spots under 
> coverslip)artifact.  We have been using fresh xylene on our stainer and 
> coverslipper, cleaned and wiped all containers dry before filling, tried 
> different lots of coverslipping film and had service on our coverslipper to 
> make sure it was functioning properly, including the xylene drip.  We 
> continue to have this artifact and it is driving us crazy.  It is sporadic 
> with no pattern of tissue type or placement on the slide.  Sometimes it lands 
> on tissue other times not.  Most of the time when we remove the coverslip and 
> re-coverslip it goes away (I am assuming because the acetone removes any 
> minute amounts of water that may be present).  We just cannot figure out 
> where the water is coming from.  Has anyone seen this artifact while using 
> the drying step on the prisma stainer?  We just recently started using the 
> drying on some slides and I am thinking maybe it is causing humidity???  I 
> cannot say for a fact that our "cornflaking" started at the same time, but it 
> is suspicious. HELP!
> 
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu 
> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Wait, Trevor 
> Jordan
> Sent: Wednesday, March 12, 2014 3:57 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Paraffin type and Tetracycline labelling Questions
> 
> For those who have done Decalcified bone processing with paraffinwhat is 
> the best type of paraffin that you guys are familiar with?
> 
> Also, if you are wanting to see a tetracycline label on the bone for bone 
> turnover, must undecalcified sections be used? How for a double tetracycline 
> label?
> 
> 
> Trevor Jordan Wait
> University of Texas Health Science Center, San Antonio Class of 2017 MD 
> Candidate Abilene Christian University Class of 2013 Graduate B.S.  
> Biochemistry ___
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> 
> ___
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[Histonet] RE: MUM-1

[Sebree Linda A]

This is our protocol for the Ultra; maybe it will help.

64" CC1, 32" incubation (MUM-1, 760-4529) @ 36 degrees, Hem II/4".  This is 
with UltraView DAB detection.  We use tonsil as well however we validated with 
HD, LN, GI, tonsil, etc.

Good luck!

Linda A. Sebree 
University of Wisconsin Hospital & Clinics 
IHC/ISH Laboratory 
600 Highland Ave. 
Madison, WI 53792 
(608)265-6596 
FAX: (608)262-7174 

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Davis, Cassie
Sent: Thursday, March 13, 2014 8:54 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] MUM-1

Good morning Histonet Folks,

 I am hoping one of you will help me. I am in the process of optimizing 
an IHC protocol on the MUM-1 antibody on paraffin tissue for the Benchmark 
XTstainer and I am not thrilled with the results I am getting. I have tried the 
"usual adjustments" and the results are less than optimal in my opinion. I am 
using a normal tonsil control right now but if you have another suggestion 
please do not hesitate to recommend. I am praying somebody might have done this 
before and would be willing to share their staining protocol or tips with this.

Cassandra Davis
cda...@che-east.org
302-575-8095



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[Histonet] RE: Cornflaking artifact

[Metzger, Kenneth]

Can anyone share the lot # on their coverslipping film (Sakura)? We are seeing 
this as well out of the blue..haven't changed a thing.

Kenneth G Metzger HTL(ASCP)
Histology Supervisor
ARUP Labs
Salt Lake City, Utah
Phone: (801)583-2787 ext. 3101
Fax: (801) 584-5244
Email: kenneth.metz...@aruplab.com


-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of HERRINGTON, 
SHEILA
Sent: Thursday, March 13, 2014 9:42 AM
To: 'Laurie Colbert'; Sharon Scalise; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Cornflaking artifact

We also have recently started to see this artifact more than ever before, and 
nothing in our process has changed.  We have tried everything to correct to no 
avail.  Wonder if it is possible to be a change in some type of supply, either 
xylene or coverslipping film.  Something has changed but am at a loss as to 
what.


Sheila Herrington
Technical Lead Histopathology and Immunohistochemistry Kelowna General Hospital
2268 Pandosy Street, Kelowna, B.C. V1Y 1T2
250-862-4300 ext 7587 or 7510
sheila.herring...@interiorhealth.ca



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert
Sent: Thursday, March 13, 2014 6:30 AM
To: Sharon Scalise; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Cornflaking artifact

You will also see the cornflaking if your tissue is lifting off of the slide at 
all.  We used to get this more often on hard, decal specimens than on other 
specimens.  We used the film to coverslip.  If you remove the film from the 
problem slides and recoverslip conventionally with extra mountant and glass 
coverslips, I'm sure you will not see the artifact.

Laurie Colbert

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sharon Scalise
Sent: Wednesday, March 12, 2014 8:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Cornflaking artifact

I am looking for help with "cornflaking" (tiny, brown dry spots under 
coverslip)artifact.  We have been using fresh xylene on our stainer and 
coverslipper, cleaned and wiped all containers dry before filling, tried 
different lots of coverslipping film and had service on our coverslipper to 
make sure it was functioning properly, including the xylene drip.  We continue 
to have this artifact and it is driving us crazy.  It is sporadic with no 
pattern of tissue type or placement on the slide.  Sometimes it lands on tissue 
other times not.  Most of the time when we remove the coverslip and 
re-coverslip it goes away (I am assuming because the acetone removes any minute 
amounts of water that may be present).  We just cannot figure out where the 
water is coming from.  Has anyone seen this artifact while using the drying 
step on the prisma stainer?  We just recently started using the drying on some 
slides and I am thinking maybe it is causing humidity???  I cannot say for a 
fact that our "cornflaking" started at the same time, but it is suspicious. 
HELP!

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Wait, Trevor 
Jordan
Sent: Wednesday, March 12, 2014 3:57 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Paraffin type and Tetracycline labelling Questions

For those who have done Decalcified bone processing with paraffinwhat is 
the best type of paraffin that you guys are familiar with?

Also, if you are wanting to see a tetracycline label on the bone for bone 
turnover, must undecalcified sections be used? How for a double tetracycline 
label?


Trevor Jordan Wait
University of Texas Health Science Center, San Antonio Class of 2017 MD 
Candidate Abilene Christian University Class of 2013 Graduate B.S.  
Biochemistry ___
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Re: [Histonet] RE: Cornflaking artifact

[srishan]

We had this problem several years ago.  We  were using the sakura tapes 
with the coverslipper. 
We did the following: 

Last three alcohols were changes frequently. 
Slides should be not dry when loading on coverslipper.
If you could load two racks at a time, only load one.  By this way the 
slides in the second rack will not dry out. 
Finally, change the tapes from sakura to Mercedes Medical tapes.

Mala

Nirmala Srishan
Holy Name Medical Center




From:   "HERRINGTON, SHEILA" 
To: 'Laurie Colbert' , Sharon Scalise 
, "histonet@lists.utsouthwestern.edu" 

Date:   03/13/2014 11:43 AM
Subject:[Histonet] RE: Cornflaking artifact
Sent by:histonet-boun...@lists.utsouthwestern.edu



We also have recently started to see this artifact more than ever before, 
and nothing in our process has changed.  We have tried everything to 
correct to no avail.  Wonder if it is possible to be a change in some type 
of supply, either xylene or coverslipping film.  Something has changed but 
am at a loss as to what.


Sheila Herrington
Technical Lead Histopathology and Immunohistochemistry
Kelowna General Hospital
2268 Pandosy Street, Kelowna, B.C. V1Y 1T2
250-862-4300 ext 7587 or 7510
sheila.herring...@interiorhealth.ca



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu [
mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie 
Colbert
Sent: Thursday, March 13, 2014 6:30 AM
To: Sharon Scalise; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Cornflaking artifact

You will also see the cornflaking if your tissue is lifting off of the 
slide at all.  We used to get this more often on hard, decal specimens 
than on other specimens.  We used the film to coverslip.  If you remove 
the film from the problem slides and recoverslip conventionally with extra 
mountant and glass coverslips, I'm sure you will not see the artifact.

Laurie Colbert

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu [
mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sharon 
Scalise
Sent: Wednesday, March 12, 2014 8:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Cornflaking artifact

I am looking for help with "cornflaking" (tiny, brown dry spots under 
coverslip)artifact.  We have been using fresh xylene on our stainer and 
coverslipper, cleaned and wiped all containers dry before filling, tried 
different lots of coverslipping film and had service on our coverslipper 
to make sure it was functioning properly, including the xylene drip.  We 
continue to have this artifact and it is driving us crazy.  It is sporadic 
with no pattern of tissue type or placement on the slide.  Sometimes it 
lands on tissue other times not.  Most of the time when we remove the 
coverslip and re-coverslip it goes away (I am assuming because the acetone 
removes any minute amounts of water that may be present).  We just cannot 
figure out where the water is coming from.  Has anyone seen this artifact 
while using the drying step on the prisma stainer?  We just recently 
started using the drying on some slides and I am thinking maybe it is 
causing humidity???  I cannot say for a fact that our "cornflaking" 
started at the same time, but it is suspicious. HELP!

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu [
mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Wait, 
Trevor Jordan
Sent: Wednesday, March 12, 2014 3:57 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Paraffin type and Tetracycline labelling Questions

For those who have done Decalcified bone processing with paraffinwhat 
is the best type of paraffin that you guys are familiar with?

Also, if you are wanting to see a tetracycline label on the bone for bone 
turnover, must undecalcified sections be used? How for a double 
tetracycline label?


Trevor Jordan Wait
University of Texas Health Science Center, San Antonio Class of 2017 MD 
Candidate Abilene Christian University Class of 2013 Graduate B.S. 
Biochemistry ___
Histonet mailing list
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[Histonet] RE: Cornflaking artifact

[HERRINGTON, SHEILA]

We also have recently started to see this artifact more than ever before, and 
nothing in our process has changed.  We have tried everything to correct to no 
avail.  Wonder if it is possible to be a change in some type of supply, either 
xylene or coverslipping film.  Something has changed but am at a loss as to 
what.


Sheila Herrington
Technical Lead Histopathology and Immunohistochemistry
Kelowna General Hospital
2268 Pandosy Street, Kelowna, B.C. V1Y 1T2
250-862-4300 ext 7587 or 7510
sheila.herring...@interiorhealth.ca



-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie Colbert
Sent: Thursday, March 13, 2014 6:30 AM
To: Sharon Scalise; histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Cornflaking artifact

You will also see the cornflaking if your tissue is lifting off of the slide at 
all.  We used to get this more often on hard, decal specimens than on other 
specimens.  We used the film to coverslip.  If you remove the film from the 
problem slides and recoverslip conventionally with extra mountant and glass 
coverslips, I'm sure you will not see the artifact.

Laurie Colbert

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sharon Scalise
Sent: Wednesday, March 12, 2014 8:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Cornflaking artifact

I am looking for help with "cornflaking" (tiny, brown dry spots under 
coverslip)artifact.  We have been using fresh xylene on our stainer and 
coverslipper, cleaned and wiped all containers dry before filling, tried 
different lots of coverslipping film and had service on our coverslipper to 
make sure it was functioning properly, including the xylene drip.  We continue 
to have this artifact and it is driving us crazy.  It is sporadic with no 
pattern of tissue type or placement on the slide.  Sometimes it lands on tissue 
other times not.  Most of the time when we remove the coverslip and 
re-coverslip it goes away (I am assuming because the acetone removes any minute 
amounts of water that may be present).  We just cannot figure out where the 
water is coming from.  Has anyone seen this artifact while using the drying 
step on the prisma stainer?  We just recently started using the drying on some 
slides and I am thinking maybe it is causing humidity???  I cannot say for a 
fact that our "cornflaking" started at the same time, but it is suspicious. 
HELP!

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Wait, Trevor 
Jordan
Sent: Wednesday, March 12, 2014 3:57 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Paraffin type and Tetracycline labelling Questions

For those who have done Decalcified bone processing with paraffinwhat is 
the best type of paraffin that you guys are familiar with?

Also, if you are wanting to see a tetracycline label on the bone for bone 
turnover, must undecalcified sections be used? How for a double tetracycline 
label?


Trevor Jordan Wait
University of Texas Health Science Center, San Antonio Class of 2017 MD 
Candidate Abilene Christian University Class of 2013 Graduate B.S.  
Biochemistry ___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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[Histonet] MUM-1

[Davis, Cassie]

Good morning Histonet Folks,

 I am hoping one of you will help me. I am in the process of optimizing 
an IHC protocol on the MUM-1 antibody on paraffin tissue for the Benchmark 
XTstainer and I am not thrilled with the results I am getting. I have tried the 
"usual adjustments" and the results are less than optimal in my opinion. I am 
using a normal tonsil control right now but if you have another suggestion 
please do not hesitate to recommend. I am praying somebody might have done this 
before and would be willing to share their staining protocol or tips with this.

Cassandra Davis
cda...@che-east.org
302-575-8095



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[Histonet] RE: Cornflaking artifact

[Laurie Colbert]

You will also see the cornflaking if your tissue is lifting off of the slide at 
all.  We used to get this more often on hard, decal specimens than on other 
specimens.  We used the film to coverslip.  If you remove the film from the 
problem slides and recoverslip conventionally with extra mountant and glass 
coverslips, I'm sure you will not see the artifact.

Laurie Colbert

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sharon Scalise
Sent: Wednesday, March 12, 2014 8:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: Cornflaking artifact

I am looking for help with "cornflaking" (tiny, brown dry spots under 
coverslip)artifact.  We have been using fresh xylene on our stainer and 
coverslipper, cleaned and wiped all containers dry before filling, tried 
different lots of coverslipping film and had service on our coverslipper to 
make sure it was functioning properly, including the xylene drip.  We continue 
to have this artifact and it is driving us crazy.  It is sporadic with no 
pattern of tissue type or placement on the slide.  Sometimes it lands on tissue 
other times not.  Most of the time when we remove the coverslip and 
re-coverslip it goes away (I am assuming because the acetone removes any minute 
amounts of water that may be present).  We just cannot figure out where the 
water is coming from.  Has anyone seen this artifact while using the drying 
step on the prisma stainer?  We just recently started using the drying on some 
slides and I am thinking maybe it is causing humidity???  I cannot say for a 
fact that our "cornflaking" started at the same time, but it is suspicious. 
HELP!

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Wait, Trevor 
Jordan
Sent: Wednesday, March 12, 2014 3:57 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Paraffin type and Tetracycline labelling Questions

For those who have done Decalcified bone processing with paraffinwhat is 
the best type of paraffin that you guys are familiar with?

Also, if you are wanting to see a tetracycline label on the bone for bone 
turnover, must undecalcified sections be used? How for a double tetracycline 
label?


Trevor Jordan Wait
University of Texas Health Science Center, San Antonio Class of 2017 MD 
Candidate Abilene Christian University Class of 2013 Graduate B.S.  
Biochemistry ___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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Re: [Histonet] RE: Cornflaking artifact

[b427297]

Is this a tape coverslipper? If so, you do have minute traces of water 
carryover to your xylene. If there is even the hint of pink in you last 
dehydrant before xylene, you will get that cornflake artifact. Acetone wont 
help, because water still be present. Increase number of absolute alcohol 
before xylene, and check often for eosin carryover. This fixed our problem. 
Jackie O'

Sent from my iPhone

> On Mar 12, 2014, at 21:59, Sharon Scalise  wrote:
> 
> I am looking for help with "cornflaking" (tiny, brown dry spots under 
> coverslip)artifact.  We have been using fresh xylene on our stainer and 
> coverslipper, cleaned and wiped all containers dry before filling, tried 
> different lots of coverslipping film and had service on our coverslipper to 
> make sure it was functioning properly, including the xylene drip.  We 
> continue to have this artifact and it is driving us crazy.  It is sporadic 
> with no pattern of tissue type or placement on the slide.  Sometimes it lands 
> on tissue other times not.  Most of the time when we remove the coverslip and 
> re-coverslip it goes away (I am assuming because the acetone removes any 
> minute amounts of water that may be present).  We just cannot figure out 
> where the water is coming from.  Has anyone seen this artifact while using 
> the drying step on the prisma stainer?  We just recently started using the 
> drying on some slides and I am thinking maybe it is causing humidity???  I 
> cannot say for a fact that our "cornflaking" started at the same time, but it 
> is suspicious. HELP!
> 
> -Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu 
> [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Wait, Trevor 
> Jordan
> Sent: Wednesday, March 12, 2014 3:57 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Paraffin type and Tetracycline labelling Questions
> 
> For those who have done Decalcified bone processing with paraffinwhat is 
> the best type of paraffin that you guys are familiar with?
> 
> Also, if you are wanting to see a tetracycline label on the bone for bone 
> turnover, must undecalcified sections be used? How for a double tetracycline 
> label?
> 
> 
> Trevor Jordan Wait
> University of Texas Health Science Center, San Antonio Class of 2017 MD 
> Candidate Abilene Christian University Class of 2013 Graduate B.S.  
> Biochemistry ___
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> ___
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