[Histonet] Re: thickness of slides
Diana McCaig asks for your protocol for cutting prostate needle core biopsies.how many spares you cut and do you designate the level on the slide label if multiple levels are submitted on one slide? I worked in one of the big prostate labs for a while, and this is what they use, and what I use when can get it. Cut onto slides suitable for immunohistochemistry. Stain slides 1, 3, and 5 with H and E. Hold slides 2 and 4 for possible immunohistochemistry, needed for at least one case in ten. I don't want more than one level on a slide, with a ribbon of at least two. When you embed, by the way, don't put more than two cores in a block, unless the cores are very short. Measure each core and record that in the gross. Make sure you can tell cases apart, since all prostate biopsies look alike in the gross. The big prostate lab used color coding of cassettes, slides, and labels, with a cycle of ten, so that (for example) if the accession number ended in -3 the cassette was always green. Bob Richmond Samurai Pathologist Asbury Place, Maryville TN ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: thickness of slides
4 microns and 3 for lymph nodes. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Diana McCaig Sent: Tuesday, September 18, 2012 2:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] thickness of slides Can you tell me what thickness you cut your routine slides for HE and immuno (in particular lymph nodes). Also, your protocol for cutting prostate needle core biopsies.how many spares you cut and do you designate the level on the slide label if multiple levels are submitted on one slide? Thanks Diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
[Histonet] RE: thickness of slides
Routine at 4, lymph nodes/bone marrows at 2, GI/prostate at 3 Prostate we cut 3 shallow levels. pick up 2 extra slides from the second level as unstained which get used for p63/PIN cocktail (p504s/p63) if requested Each slide has one level...2 sections per slide Vanessa -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Diana McCaig Sent: Tuesday, September 18, 2012 1:00 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] thickness of slides Can you tell me what thickness you cut your routine slides for HE and immuno (in particular lymph nodes). Also, your protocol for cutting prostate needle core biopsies.how many spares you cut and do you designate the level on the slide label if multiple levels are submitted on one slide? Thanks Diana ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
