[Histonet] RE: Water in molds

2013-01-29 Thread HERRINGTON, SHEILA
Are you using mold release?  Sometimes that can cause what you are describing.  

Sheila Herrington, RT (CSMLS)

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bonnie 
Gambichler
Sent: Tuesday, January 29, 2013 3:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Water in molds

Hello everyone,

My question concerns the possible presence of water in the embedding molds.
Lately I've been noticing that the sections made from blocks embedded in our 
lab are melting very quickly around the edges.  The sections are also coming 
apart from each other when placed on the waterbath.  The tissue remains intact, 
so I don't think it's a processing problem.  Could this be due to residual 
water left in the mold mixing with the paraffin?

Bonnie Gambichler, HT (ASCP)
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[Histonet] RE: Water in molds

2013-01-29 Thread Roger Heyna
We've seen this problem before in our lab, and it was traced back to the mold 
release, just as Sheila said.
 
Roger Heyna, BS, HTL
Loyola University Medical Center

 HERRINGTON, SHEILA sheila.herring...@interiorhealth.ca 1/29/2013 5:08 
 PM 
Are you using mold release?  Sometimes that can cause what you are describing.  

Sheila Herrington, RT (CSMLS)

-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu 
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bonnie 
Gambichler
Sent: Tuesday, January 29, 2013 3:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Water in molds

Hello everyone,

My question concerns the possible presence of water in the embedding molds.
Lately I've been noticing that the sections made from blocks embedded in our 
lab are melting very quickly around the edges.  The sections are also coming 
apart from each other when placed on the waterbath.  The tissue remains intact, 
so I don't think it's a processing problem.  Could this be due to residual 
water left in the mold mixing with the paraffin?

Bonnie Gambichler, HT (ASCP)
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