[Histonet] vanGieson-Trichrome staining protocol
Is there anyone out there that has done these two stains together? If so, I need assistance with a protocol. Also I am doing a reticulum stain and I am having a time with the ammoniacal silver solution, I can get the solution clear but when it comes to the cloudy do they mean milky cloudy? Thank you Adele ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] vanGieson-Trichrome staining protocol
Adele, Since the vanGieson IS a trichrome stain, why would anyone wish to combine one trichrome with another? Bryan - Original Message - From: Doughty, Adele adoug...@lrri.org To: histonet@lists.utsouthwestern.edu Sent: Monday, July 19, 2010 10:29 AM Subject: [Histonet] vanGieson-Trichrome staining protocol Is there anyone out there that has done these two stains together? If so, I need assistance with a protocol. Also I am doing a reticulum stain and I am having a time with the ammoniacal silver solution, I can get the solution clear but when it comes to the cloudy do they mean milky cloudy? Thank you Adele ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Re: [Histonet] vanGieson-Trichrome staining protocol
Are you perhaps refering to the Verhoff's Van Gieson, if you are trying to stain elastic then a trichrome it can be done. The silver should be cloudy like a slightly dirty window , not milky generally if you add enough ammonia to the silver and sodium hydroxide mixture to render it completely clear then you will probably have added too much ammonia. Rena Fail - Original Message From: Doughty, Adele adoug...@lrri.org To: histonet@lists.utsouthwestern.edu Sent: Mon, July 19, 2010 10:29:19 AM Subject: [Histonet] vanGieson-Trichrome staining protocol Is there anyone out there that has done these two stains together? If so, I need assistance with a protocol. Also I am doing a reticulum stain and I am having a time with the ammoniacal silver solution, I can get the solution clear but when it comes to the cloudy do they mean milky cloudy? Thank you Adele ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
RE: [Histonet] vanGieson-Trichrome staining protocol
And it looks so pretty (VVG/TRI)- especially on lung. Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rena Fail Sent: Monday, July 19, 2010 12:47 PM To: Doughty, Adele; histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] vanGieson-Trichrome staining protocol Are you perhaps refering to the Verhoff's Van Gieson, if you are trying to stain elastic then a trichrome it can be done. The silver should be cloudy like a slightly dirty window , not milky generally if you add enough ammonia to the silver and sodium hydroxide mixture to render it completely clear then you will probably have added too much ammonia. Rena Fail - Original Message From: Doughty, Adele adoug...@lrri.org To: histonet@lists.utsouthwestern.edu Sent: Mon, July 19, 2010 10:29:19 AM Subject: [Histonet] vanGieson-Trichrome staining protocol Is there anyone out there that has done these two stains together? If so, I need assistance with a protocol. Also I am doing a reticulum stain and I am having a time with the ammoniacal silver solution, I can get the solution clear but when it comes to the cloudy do they mean milky cloudy? Thank you Adele ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet