The PiMS team intends that the CCP4 records link not only with the
synchrotron, but further back to crystallogenesis records in xtalPiMS,
and protein production records in PiMS. The benefits this will provide
include:
- if you find an unexpected piece of electron density, navigating to
records
Dear all
I have a molecular replacement problem and I really need your help
with this one. My protein crystallized in C2 instead of P21 like it
did before. Now when I tried to do MR to place it in C2 it doesn't
work, I get no solution. This protein has been crystallized in
P212121, P31,
When doing MR, I usually try Phaser and EPMR.
Phaser rarely fails for a high-homology MR search, but can have
difficulty fitting 3 or more protein units in the ASU. EPMR is a
little better, in my experience, in ferreting out a solution for 3
or more protein
Dear all
The P21 unit cell is a=86.499 b=38.554 c=94.027 al=90.0 be=97.92 ga=90.0
The C2 unit cell is a=103.932 b=62.253 c=96.305 al=90.0 be=111.934
ga=90.0
Phaser and the other programs do give me solutions but the LLG in
Phaser is about -1000's and the highest TFZ score I get is 4.9, and
Hello,
You could try Disulfide by Design:
http://cptweb.cpt.wayne.edu/DbD/
Good luck,
Konstantin
On Mon, 23 Aug 2010, Jacob Keller wrote:
Dear Crystallographers,
I remember having heard of a program which takes a given oligomeric assembly,
and suggests optimum disulfides to stablize the
I think that Disulfide by Design, or DbD, is geared toward monomeric proteins
(ie. for enhancing stability). I'm sure you could get it to work with an
oligomer with a little tinkering, but there is another server called sGAL that
may be more like what you're looking for:
