Dear all users,
I got my crystals at the bottom of the tube on ice just before I started to
screen crystals. Those crystals' shapes were good but easy to melt when I
harvested them. I shot these crystals. They only diffracted to 7A to 8A. Has
anyone ever met this situation before? How to impr
Hi Lingyuan,
1) you're very lucky (but you already know that)
2) crystals grown quickly in bulk rarely diffract well. I would follow up
by a conventional droplet crystallization against a concentrating agent -
in the cold - such that your protein gradually concentrates from [X] to 2-3
times [X] an
Yes, it is many years ago, a large protein batch (50 ml or so) stored in
the cold room that produced an impressive layer of crystals at the
bottom of the flask.
One student at that time tested many crystals and found only a few that
diffracted to atomic (!) resolution.
Crystallization is a
[mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of kiki
Sent: 18 October 2016 11:42
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Crystals grow at the bottom of the tube.
Dear all users,
I got my crystals at the bottom of the tube on ice just before I started to
screen crystals. Those crystals' shapes
t;
>
>
> Best wishes,
>
>
>
> David
>
>
>
> *From:* CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] *On Behalf Of *
> kiki
> *Sent:* 18 October 2016 11:42
> *To:* CCP4BB@JISCMAIL.AC.UK
> *Subject:* [ccp4bb] Crystals grow at the bottom of the tube.
>
>
