Hi Sam,
The effect of reducing agents like beta-ME, DTT or TCEP is exactly as you say,
the prevention of disulphide formation or cleavage of existing disulphide
bridges and therefore prevention of aggregation if inter-molecular disulphide
bridges can be formed.
A balancing of reducing power, or
Dear all,
Can anyone enlighten me the effect of reducer in crystallography?
I understand that it removes disulphide bond, and prevent protein
aggregation. But how do we find a balance point and must we remove it before
screening crystals?
Thanks for answering first.
Cheers
sam