Hi, thank you for you reply. Could you tell me if you try to dye membrane
protein crystal with detergent?
Thanks.
Y.B.
2010-11-18
yybbll
发件人： Matthew Bratkowski
发送时间： 2010-11-17 02:58:40
收件人： CCP4BB
抄送：
主题： Re: [ccp4bb] how to optimize small rod-shaped crystals
I like using
will still give you false positives.
If you need bigger crystals, try to slow down the nucleation (use lower
temperature, different ratio of protein:crystallant, etc).
Clement
From: yybbll
Sent: Wednesday, November 17, 2010 2:42 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] how to optimize
fide protein crystals and a negative control with bona fide salt crystals.
>>
>>
>> From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of
>> Matthew Bratkowski
>> Sent: Tuesday, November 16, 2010 7:58 PM
>> To: CCP4BB
> *From:* CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] *On Behalf Of
> *Matthew
> Bratkowski
> *Sent:* Tuesday, November 16, 2010 7:58 PM
> *To:* CCP4BB@JISCMAIL.AC.UK
> *Subject:* Re: [ccp4bb] how to optimize small rod-shaped crystals
>
> I like using Izit dye from
lower
temperature, different ratio of protein:crystallant, etc).
Clement
From: yybbll
Sent: Wednesday, November 17, 2010 2:42 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] how to optimize small rod-shaped crystals
Hi, everybody,
I try to crystallize one membrane protein. All crystals were
: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] how to optimize small rod-shaped crystals
I like using Izit dye from Hampton
(http://hamptonresearch.com/product_detail.aspx?cid=4
<http://hamptonresearch.com/product_detail.aspx?cid=4&sid=41&pid=33>
&sid=41&pid=33) to check if c
I like using Izit dye from Hampton (
http://hamptonresearch.com/product_detail.aspx?cid=4&sid=41&pid=33) to check
if crystals are protein or salt. If the crystals are protein, the dye
should absorb rather readily into the crystals and turn them blue, while the
rest of the drop will eventually turn
If you have a polarizer on your microscope you could see if they are extremely
dichroic, in which case they may be salt. You could also open the well up and
see if they are heavy - do they sink immediately when you lift them to the top
of the drop? This could also indicate salt. But I agree with
Hi Yibin,
MiTeGen offers different loops that make it easier to pick really tiny crystals
which can be diffracted at the synchrotron.
If you would like to optimize small needles or rod shaped crystals, you may try
an additive screen.
You may also try to lower the rate of crystal growth.
Perha
Hi Yibin,
I cannot tell the actual scale of your crystals but judging by the curve of the
drop outline, these crystals look to be plenty big enough to mount in a loop to
stick in the beam and test diffraction. If indeed they are too small to mount a
single crystal, and you are mainly intereste
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